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MicroRNA-directed cleavage of targets: mechanism and experimental approaches

박준현|신찬석
생화학분자생물학회 (Korean Society Of Biochemistry And Molecular Biology)|2014.08|BMB Reports|제47권 제8호|pp. 417-423

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참고문헌

키워드

MicroRNAArgonaute proteinMicroRNA-directed cleavage
주제분류화학

초록

MicroRNAs (miRNAs) are a large family of post-transcriptionalregulators, which are 21-24 nt in length and play a role in awide variety of biological processes in eukaryotes. The pastfew years have seen rapid progress in our understanding ofmiRNA biogenesis and the mechanism of action, whichcommonly entails a combination of target degradation andtranslational repression. The target degradation mediated byArgonaute-catalyzed endonucleolytic cleavage exerts asignificant repressive effect on target mRNA expression,particularly during rapid developmental transitions. Thisreview outlines the current understanding of the mechanisticaspects of this important process and discusses severaldifferent experimental approaches to identify miRNA cleavage targets.

Abstract

MicroRNAs (miRNAs) are a large family of post-transcriptionalregulators, which are 21-24 nt in length and play a role in awide variety of biological processes in eukaryotes. The pastfew years have seen rapid progress in our understanding ofmiRNA biogenesis and the mechanism of action, whichcommonly entails a combination of target degradation andtranslational repression. The target degradation mediated byArgonaute-catalyzed endonucleolytic cleavage exerts asignificant repressive effect on target mRNA expression,particularly during rapid developmental transitions. Thisreview outlines the current understanding of the mechanisticaspects of this important process and discusses severaldifferent experimental approaches to identify miRNA cleavage targets.

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