Secondary structure of the Irf7 5’-UTR, analyzed using SHAPE (selective 2’-hydroxyl acylation analyzed by primer extension)
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
OASL1 is a member of the 2’-5’-oligoadenylate synthetase(OAS) family and promotes viral clearance by activating RNaseL. OASL1 interacts with the 5’-untranslated region (UTR) ofinterferon regulatory factor 7 (Irf7) and inhibits its translation. To identify the secondary structure required for OASL1binding, we examined the 5’-UTR of the Irf7 transcript using“selective 2’-hydroxyl acylation analyzed by primer extension”(SHAPE). SHAPE takes advantage of the selective acylation ofresidues in single-stranded regions by 1-methyl-7-nitroisatoicanhydride (1M7). We found five major acylation sites locatedin, or next to, predicted single-stranded regions of the Irf75’-UTR. These results demonstrate the involvement of the stemstructure of the Irf7 5’-UTR in the regulation of Irf7 translation,mediated by OASL1.
Abstract
OASL1 is a member of the 2’-5’-oligoadenylate synthetase(OAS) family and promotes viral clearance by activating RNaseL. OASL1 interacts with the 5’-untranslated region (UTR) ofinterferon regulatory factor 7 (Irf7) and inhibits its translation. To identify the secondary structure required for OASL1binding, we examined the 5’-UTR of the Irf7 transcript using“selective 2’-hydroxyl acylation analyzed by primer extension”(SHAPE). SHAPE takes advantage of the selective acylation ofresidues in single-stranded regions by 1-methyl-7-nitroisatoicanhydride (1M7). We found five major acylation sites locatedin, or next to, predicted single-stranded regions of the Irf75’-UTR. These results demonstrate the involvement of the stemstructure of the Irf7 5’-UTR in the regulation of Irf7 translation,mediated by OASL1.
참고문헌 (21)
- Akira, S., "Pathogen recognition and innate immunity", Cell, 124, pp.783~801 (2006)
- Lee, M. S., "Signaling pathways downstream of pattern-recognition receptors and their cross talk", Annu. Rev. Biochem, 76, pp.447~480 (2007)
- Lee, M. S., "OASL1 inhibits translation of the type I interferon-regulating transcription factor Irf7", Nat. Immunol, 14, pp.346~355 (2013)
- Taniguchi, T., "IRF family of transcription factors as regulators of host defense", Annu. Rev. Immunol, 19, pp.623~655 (2001)
- Takaoka, A., "Interferon regulatory factor family of transcription factors and regulation of oncogenesis", Cancer Sci, 99, pp.467~478 (2008)
- Honda, K., "IRF-7 is the master regulator of type-I interferon-dependent immune responses", Nature, 434, pp.772~777 (2005)
- Ning, S., "Irf7: activation, regulation, modification and function", Genes Immun, 12, pp.399~414 (2011)
- Sato, M., "Positive feedback regulation of type I IFN genes by the IFN-inducible transcription factor IRF-7", FEBS Lett, 441, pp.106~110 (1998)
- Kertesz, M., "Genome-wide measurement of RNA secondary structure in yeast", Nature, 467, pp.103~107 (2010)
- Wan, Y., "Genome-wide mapping of RNA structure using nuclease digestion and high-throughput sequencing", Nat. Protoc, 8, pp.849~869 (2013)
- Mortimer, S. A., "A fast-acting reagent for accurate analysis of RNA secondary and tertiary structure by SHAPE chemistry", J. Am. Chem. Soc, 129, pp.4144~4145 (2007)
- Lucks, J. B., "Multiplexed RNA structure characterization with selective 2'-hydroxyl acylation analyzed by primer extension sequencing (SHAPE-Seq)", Proc. Natl. Acad. Sci. U. S. A, 108, pp.11063~11068 (2011)
- Wang, Z., "The cap-binding translation initiation factor, eIF4E, binds a pseudoknot in a viral cap-independent translation element", Structure, 19, pp.868~880 (2011)
- Low, J. T., "SHAPE-directed RNA secondary structure prediction", Methods, 52, pp.150~158 (2010)
- Bevilacqua, A., "Post-transcriptional regulation of gene expression by degradation of messenger RNAs", J. Cell. Physiol, 195, pp.356~372 (2003)
- Turner, R., "Safer one-pot synthesis of the 'SHAPE' reagent 1-methyl-7-nitroisatoic anhydride (1m7)", RNA, 19, pp.1857~1863 (2013)
- Wilkinson, K. A., "Influence of nucleotide identity on ribose 2'-hydroxyl reactivity in RNA", RNA, 15, pp.1314~1321 (2009)
- Deigan, K. E., "Accurate SHAPE-directed RNA structure determination", Proc. Natl. Acad. Sci. U. S. A, 106, pp.97~102 (2009)
- Merino, E. J., "RNA structure analysis at single nucleotide resolution by selective 2'-hydroxyl acylation and primer extension (SHAPE)", J. Am. Chem. Soc, 127, pp.4223~4231 (2005)
- Gherghe, C. M., "Strong correlation between SHAPE chemistry and the generalized NMR order parameter (S2) in RNA", J. Am. Chem. Soc, 130, pp.12244~12245 (2008)
- Tijerina, P., "DMS footprinting of structured RNAs and RNA-protein complexes", Nat. Protoc, 2, pp.2608~2623 (2007)