Local protein synthesis in neuronal axons: why and how we study
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Adaptive brain function and synaptic plasticity rely on dynamicregulation of local proteome. One way for the neuronto introduce new proteins to the axon terminal is to transportthose from the cell body, which had long been thought as theonly source of axonal proteins. Another way, which is thetopic of this review, is synthesizing proteins on site by localmRNA translation. Recent evidence indicates that the axonstores a reservoir of translationally silent mRNAs and regulatestheir expression solely by translational control. Different stimulito axons, such as guidance cues, growth factors, and nerve injury,promote translation of selective mRNAs, a process requiredfor the axon’s ability to respond to these cues. One of thecritical questions in the field of axonal protein synthesis is howmRNA-specific local translation is regulated by extracellularcues. Here, we review current experimental techniques that canbe used to answer this question. Furthermore, we discuss hownew technologies can help us understand what biological processesare regulated by axonal protein synthesis in vivo.
Abstract
Adaptive brain function and synaptic plasticity rely on dynamicregulation of local proteome. One way for the neuronto introduce new proteins to the axon terminal is to transportthose from the cell body, which had long been thought as theonly source of axonal proteins. Another way, which is thetopic of this review, is synthesizing proteins on site by localmRNA translation. Recent evidence indicates that the axonstores a reservoir of translationally silent mRNAs and regulatestheir expression solely by translational control. Different stimulito axons, such as guidance cues, growth factors, and nerve injury,promote translation of selective mRNAs, a process requiredfor the axon’s ability to respond to these cues. One of thecritical questions in the field of axonal protein synthesis is howmRNA-specific local translation is regulated by extracellularcues. Here, we review current experimental techniques that canbe used to answer this question. Furthermore, we discuss hownew technologies can help us understand what biological processesare regulated by axonal protein synthesis in vivo.
참고문헌 (40)
- Peters A, "Fine Structure of the Nervous System: Neurons and Their Supporting Cells" (1971), Oxford University Print
- Steward O, "Preferential localization of polyribosomes under the base of dendritic spines in granule cells of the dentate gyrus", J Neurosci, 2, pp.284~291 (1982)
- Sutton MA, "Dendritic protein synthesis, synaptic plasticity, and memory", Cell, 127, pp.49~58 (2006)
- Steward O, "Polyribosomes associated with synaptic specializations on axon initial segments: localization of protein-synthetic machinery at inhibitory synapses", J Neurosci, 6, pp.3079~3085 (1986)
- Yamada KM, "Ultrastructure and function of growth cones and axons of cultured nerve cells", J Cell Biol, 49, pp.614~635 (1971)
- Tennyson VM, "The fine structure of the axon and growth cone of the dorsal root neuroblast of the rabbit embryo", J Cell Biol, 44, pp.62~79 (1970)
- Zelena J, "Ribosome-like particles in myelinated axons of the rat", Brain Res, 24, pp.359~363 (1970)
- Campbell DS, "Chemotropic responses of retinal growth cones mediated by rapid local protein synthesis and degradation", Neuron, 32, pp.1013~1026 (2001)
- Zhang X, "Localized synaptic potentiation by BDNF requires local protein synthesis in the developing axon", Neuron, 36, pp.675~688 (2002)
- Jung H, "Axonal mRNA localization and local protein synthesis in nervous system assembly, maintenance and repair", Nat Rev Neurosci, 13, pp.308~324 (2012)
- Minis A, "Subcellular transcriptomics-dissection of the mRNA composition in the axonal compartment of sensory neurons", Dev Neurobiol, 74, pp.365~381 (2014)
- Andreassi C, "An NGF-responsive element targets myo-inositol monophosphatase-1 mRNA to sympathetic neuron axons", Nat Neurosci, 13, pp.291~301 (2010)
- Gumy LF, "Transcriptome analysis of embryonic and adult sensory axons reveals changes in mRNA repertoire localization", RNA, 17, pp.85~98 (2011)
- Taylor AM, "Axonal mRNA in uninjured and regenerating cortical mammalian axons", J Neurosci, 29, pp.4697~4707 (2009)
- Zivraj KH, "Subcellular profiling reveals distinct and developmentally regulated repertoire of growth cone mRNAs", J Neurosci, 30, pp.15464~15478 (2010)
- Willis D, "Differential transport and local translation of cytoskeletal, injury-response, and neurodegeneration protein mRNAs in axons", J Neurosci, (25), pp.778~791 (2005)
- Yoon BC, "Local Translation of Extranuclear Lamin B Promotes Axon Maintenance", Cell, 148, pp.1~13 (2012)
- Campenot RB, "Local control of neurite development by nerve growth factor", Proc Natl Acad Sci U S A, 74, pp.4516~4519 (1977)
- Pazyra-Murphy MF, "Preparation and maintenance of dorsal root ganglia neurons in compartmented cultures", J Vis Exp, 20, pp.951~ (2008)
- Boyden S, "The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes", J Exp Med, 115, pp.453~466 (1962)
- Willis DE, "Profiling axonal mRNA transport", Methods Mol Biol, 714, pp.335~352 (2011)
- Taylor AM, "A microfluidic culture platform for CNS axonal injury, regeneration and transport", Nat Methods, 2, pp.599~605 (2005)
- Emmert-Buck MR, "Laser capture microdissection", Science, 274, pp.998~1001 (1996)
- Dieterich DC, "Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging(BONCAT)", Proc Natl Acad Sci U S A, 103, pp.9482~9487 (2006)
- Ong SE, "Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics", Mol Cell Proteomics, 1, pp.376~386 (2002)
- Schiapparelli LM, "Direct detection of biotinylated proteins by mass spectrometry", J Proteome Res, 13, pp.3966~3978 (2014)
- Aviner R, "Genomewide identification and quantification of protein synthesis in cultured cells and whole tissues by puromycin-associated nascent chain proteomics(PUNCH-P)", Nat Protoc, 9, pp.751~760 (2014)
- Heiman M, "A translational profiling approach for the molecular characterization of CNS cell types", Cell, 135, pp.738~748 (2008)
- Darnell JC, "FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism", Cell, 146, pp.247~261 (2011)
- Ingolia NT, "Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling", Science, 324, pp.218~223 (2009)
- Aakalu G, "Dynamic visualization of local protein synthesis in hippocampal neurons", Neuron, 30, pp.489~502 (2001)
- Leung KM, "Live visualization of protein synthesis in axonal growth cones by microinjection of photoconvertible Kaede into Xenopus embryos", Nat Protoc, 3, pp.1318~1327 (2008)
- Jung H, "Local translation of mRNAs in neural development", Wiley Interdiscip Rev RNA, 2, pp.153~165 (2011)
- Wang J, "Reversible glutathionylation regulates actin polymerization in A431 cells", J Biol Chem, 276, pp.47763~47766 (2001)
- Karakozova M, "Arginylation of beta-actin regulates actin cytoskeleton and cell motility", Science, 313, pp.192~196 (2006)
- Elliott TS, "Proteome labeling and protein identification in specific tissues and at specific developmental stages in an animal", Nat Biotechnol, 32, pp.465~472 (2014)
- Je HS, "Presynaptic protein synthesis required for NT-3-induced long-term synaptic modulation", Mol Brain, 4, pp.1~ (2011)
- Je HS, "Chemically inducible inactivation of protein synthesis in genetically targeted neurons", J Neurosci, 29, pp.6761~6766 (2009)
- Dmochowski IJ, "Taking control of gene expression with light-activated oligonucleotides", Biotechniques, 43, pp.161~163 (2007)
- Perry RB, "Subcellular knockout of importin beta1 perturbs axonal retrograde signaling", Neuron, 75, pp.294~305 (2012)