Primary astrocytic mitochondrial transplantation ameliorates ischemic stroke
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Mitochondria are important organelles that regulate adenosinetriphosphate production, intracellular calcium buffering, cellsurvival, and apoptosis. They play therapeutic roles in injuredcells via transcellular transfer through extracellular vesicles,gap junctions, and tunneling nanotubes. Astrocytes can secretenumerous factors known to promote neuronal survival,synaptic formation, and plasticity. Recent studies have demonstratedthat astrocytes can transfer mitochondria to damagedneurons to enhance their viability and recovery. In this study,we observed that treatment with mitochondria isolated fromrat primary astrocytes enhanced cell viability and amelioratedhydrogen peroxide-damaged neurons. Interestingly, isolated astrocyticmitochondria increased the number of cells under damagedneuronal conditions, but not under normal conditions,although the mitochondrial transfer efficiency did not differbetween the two conditions. This effect was also observed aftertransplanting astrocytic mitochondria in a rat middle cerebralartery occlusion model. These findings suggest that mitochondriatransfer therapy can be used to treat acute ischemic strokeand other diseases.
Abstract
Mitochondria are important organelles that regulate adenosinetriphosphate production, intracellular calcium buffering, cellsurvival, and apoptosis. They play therapeutic roles in injuredcells via transcellular transfer through extracellular vesicles,gap junctions, and tunneling nanotubes. Astrocytes can secretenumerous factors known to promote neuronal survival,synaptic formation, and plasticity. Recent studies have demonstratedthat astrocytes can transfer mitochondria to damagedneurons to enhance their viability and recovery. In this study,we observed that treatment with mitochondria isolated fromrat primary astrocytes enhanced cell viability and amelioratedhydrogen peroxide-damaged neurons. Interestingly, isolated astrocyticmitochondria increased the number of cells under damagedneuronal conditions, but not under normal conditions,although the mitochondrial transfer efficiency did not differbetween the two conditions. This effect was also observed aftertransplanting astrocytic mitochondria in a rat middle cerebralartery occlusion model. These findings suggest that mitochondriatransfer therapy can be used to treat acute ischemic strokeand other diseases.
참고문헌 (25)
- Paul S, "Emerging neuroprotective strategies for the treatment of ischemic stroke : an overview of clinical and preclinical studies", Exp Neurol, 335, pp.113518~ (2021)
- Bakthavachalam P, "Mitochondrial dysfunction-silent killer in cerebral ischemia", J Neurol Sci, 375, pp.417~423 (2017)
- Nunnari J, "Mitochondria : in sickness and in health", Cell, 148, pp.1145~1159 (2012)
- Karbowski M, "Neurodegeneration as a consequence of failed mitochondrial maintenance", Acta Neuropathol, 123, pp.157~171 (2012)
- McBride HM, "Mitochondria : more than just a powerhouse", Curr Biol, 16, pp.R551~R560 (2006)
- Fecher C, "Cell-typespecific profiling of brain mitochondria reveals functional and molecular diversity", Nat Neurosci, 22, pp.1731~1742 (2019)
- Chang CY, "Current progress of mitochondrial transplantation that promotes neuronal regeneration", Transl Neurodegener, 8, pp.17~ (2019)
- Konari N, "Mitochondria transfer from mesenchymal stem cells structurally and functionally repairs renal proximal tubular epithelial cells in diabetic nephropathy in vivo", Sci Rep, 9, pp.5184~ (2019)
- Pourmohammadi-Bejarpasi Z, "Mesenchymal stem cells-derived mitochondria transplantation mitigates I", R-induced injury, abolishes I, Brain Res Bull, pp.2020, 165[DOI]
- Robicsek O, "Isolated mitochondria transfer improves neuronal differentiation of schizophrenia-derived induced pluripotent stem cells and rescues deficits in a rat model of the disorder", Schizophr Bull, 44, pp.432~442 (2018)
- Freeman MR, "Evolving concepts of gliogenesis : a look way back and ahead to the next 25years", Neuron, 80, pp.613~623 (2013)
- Verkhratsky A, "Astrocytes as secretory cells of the central nervous system : idiosyncrasies of vesicular secretion", EMBO J, 35, pp.239~257 (2016)
- Jones EV, "Astrocyte-secreted matricellular proteins in CNS remodelling during development and disease", Neural Plast, 2014, pp.321209~ (2014)
- Hayakawa K, "Transfer of mitochondria from astrocytes to neurons after stroke", Nature, 535, pp.551~555 (2016)
- Nistico R, "Neuroprotective effect of hydrogen peroxide on an in vitro model of brain ischaemia", Br J Pharmacol, 153, pp.1022~1029 (2008)
- Mut M, "Hydrogen peroxide-induced stroke : elucidation of the mechanism in vivo", J Neurosurg, 110, pp.94~100 (2009)
- Cabreira V, "Parkinson’s disease : clinical review and update", Acta Med Port, 32, pp.661~670 (2019)
- Jankovic J, "Therapies in Parkinson’s disease", Curr Opin Neurol, 25, pp.433~447 (2012)
- Park CH, "Differential actions of the proneural genes encoding Mash1 and neurogenins in Nurr1-induced dopamine neuron differentiation", J Cell Sci, 119, pp.2310~2320 (2006)
- Ding YM, "Effects of 6-hydroxydopamine on primary cultures of substantia nigra : specific damage to dopamine neurons and the impact of glial cell line-derived neurotrophic factor", J Neurochem, 89, pp.776~787 (2004)
- Lightowlers RN, "Mitochondrial transplantation-a possible therapeutic for mitochondrial dysfunction? : mitochondrial transfer is a potential cure for many diseases but proof of efficacy and safety is still lacking", EMBO Rep, 21, pp.e50964~ (2020)
- Yang JL, "Diverse roles of mitochondria in ischemic stroke", Redox Biol, 16, pp.263~275 (2018)
- He Z, "Mitochondria as a therapeutic target for ischemic stroke", Free Radic Biol Med, 146, pp.45~58 (2020)
- Berridge MV, "Mitochondrial transfer from astrocytes to neurons following ischemic insult : guilt by association?", Cell Metab, 24, pp.376~378 (2016)
- 문효은, "Mitochondrial Dysfunction in Parkinson's Disease", Experimental Neurobiology, 24(2), pp.103~116 (2015), 한국뇌신경과학회