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Therapeutic potential of BMSC-conditioned medium in an in vitro model of renal fibrosis using the RPTEC/TERT1 cell line

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We investigated the therapeutic potential of bone marrow-derived mesenchymal stem cell-conditioned medium (BMSC-CM)on immortalized renal proximal tubule epithelial cells (RPTEC/TERT1) in a fibrotic environment. To replicate the increasedstiffness characteristic of kidneys in chronic kidney disease, weutilized polyacrylamide gel platforms. A stiff matrix was shownto increase α-smooth muscle actin (α-SMA) levels, indicatingfibrogenic activation in RPTEC/TERT1 cells. Interestingly, treatment with BMSC-CM resulted in significant reductions in thelevels of fibrotic markers (α-SMA and vimentin) and increases inthe levels of the epithelial marker E-cadherin and aquaporin 7,particularly under stiff conditions. Furthermore, BMSC-CM modified microRNA (miRNA) expression and reduced oxidative stresslevels in these cells. Our findings suggest that BMSC-CM canmodulate cellular morphology, miRNA expression, and oxidativestress in RPTEC/TERT1 cells, highlighting its therapeutic potentialin fibrotic kidney disease.

Abstract

We investigated the therapeutic potential of bone marrow-derived mesenchymal stem cell-conditioned medium (BMSC-CM)on immortalized renal proximal tubule epithelial cells (RPTEC/TERT1) in a fibrotic environment. To replicate the increasedstiffness characteristic of kidneys in chronic kidney disease, weutilized polyacrylamide gel platforms. A stiff matrix was shownto increase α-smooth muscle actin (α-SMA) levels, indicatingfibrogenic activation in RPTEC/TERT1 cells. Interestingly, treatment with BMSC-CM resulted in significant reductions in thelevels of fibrotic markers (α-SMA and vimentin) and increases inthe levels of the epithelial marker E-cadherin and aquaporin 7,particularly under stiff conditions. Furthermore, BMSC-CM modified microRNA (miRNA) expression and reduced oxidative stresslevels in these cells. Our findings suggest that BMSC-CM canmodulate cellular morphology, miRNA expression, and oxidativestress in RPTEC/TERT1 cells, highlighting its therapeutic potentialin fibrotic kidney disease.

참고문헌 (36)

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