Effect of 850 nm photobiomodulation on the adenosine diphosphate/adenosine triphosphate measure of apoptosis in geniculate ganglion neuronal cells in vitro
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Background: Photobiomodulation therapy (PBMT) is a non-invasive treatment that uses low-level light therapy to stimulate the cellular function and promote tissue healing. The geniculate ganglion is a complex network of nerve fibers that plays a vital role in the sensory processing of the head and face, including taste perception. A dysfunction of geniculate ganglion cells can result in taste disorders and other conditions, such as facial nerve palsy and tinnitus. Methods: This in vitro study examined the effects of PBMT on geniculate ganglion cells. Geniculate ganglion neuronal cells (GGNs) were cultured and exposed to different parameters of PBMT. The cell viability, proliferation, and adenosine diphosphate/adenosine triphosphate (ADP/ATP) levels were assessed. Results: PBMT at 10 J increased the cell viability three and seven days after PBMT via an MTT assay. The ADP/ATP assay results showed a significant difference in the ADP/ATP ratio of PBM-treated cells to the control group the days after PBM treatment. PBMT at doses of 1 and 5 J showed a significant increase in the ADP/ATP ratio compared with the control group, while the 10 J group exhibited a marked difference relative to other groups. Conclusion: The PBM treatment of 10 J improved the cell viability significantly in the GGNs and prevented apoptosis. Therefore, PBMT may be a safe and non-invasive approach for treating the geniculate ganglion, potentially addressing taste dysfunction. The study provides valuable insights into the potential therapeutic applications of PBMT at an appropriate energy level for taste or neurological diseases involving geniculate ganglion tissue.
Abstract
Background: Photobiomodulation therapy (PBMT) is a non-invasive treatment that uses low-level light therapy to stimulate the cellular function and promote tissue healing. The geniculate ganglion is a complex network of nerve fibers that plays a vital role in the sensory processing of the head and face, including taste perception. A dysfunction of geniculate ganglion cells can result in taste disorders and other conditions, such as facial nerve palsy and tinnitus. Methods: This in vitro study examined the effects of PBMT on geniculate ganglion cells. Geniculate ganglion neuronal cells (GGNs) were cultured and exposed to different parameters of PBMT. The cell viability, proliferation, and adenosine diphosphate/adenosine triphosphate (ADP/ATP) levels were assessed. Results: PBMT at 10 J increased the cell viability three and seven days after PBMT via an MTT assay. The ADP/ATP assay results showed a significant difference in the ADP/ATP ratio of PBM-treated cells to the control group the days after PBM treatment. PBMT at doses of 1 and 5 J showed a significant increase in the ADP/ATP ratio compared with the control group, while the 10 J group exhibited a marked difference relative to other groups. Conclusion: The PBM treatment of 10 J improved the cell viability significantly in the GGNs and prevented apoptosis. Therefore, PBMT may be a safe and non-invasive approach for treating the geniculate ganglion, potentially addressing taste dysfunction. The study provides valuable insights into the potential therapeutic applications of PBMT at an appropriate energy level for taste or neurological diseases involving geniculate ganglion tissue.
참고문헌 (21)
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