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구강내 미생물에 대한 서양산 고추냉이( Armoracia rusticana) 뿌리 추출물의 항균효과

The antimicrobial effect of horseradish( Armoracia rusticana)root extracts against oral pathogens

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주제분류치의학

초록

서양산 고추냉이( Armoracia rusticana)는 향신료로서의 역할뿐만 아니라 항균 효과로도 주목을 받고 있다. 고추냉이의 항균성분은 isothiocyanate류로 그 중에서도 allyl isothiocyanate(AIT)가 항균활성이 강한 것으로 알려져 있다. 본 연구에서는 최소억제농도와 최소살균농도를 측정하여 다양한 구강내 미생물( Streptococcus mutans ATCC 25175, Streptococcus sobrinus(d) ATCC 27607, Lactobacillus casei ATCC 393, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Actinobacillus actimomycetemcomitans ATCC 29522, Candida albicans ATCC 10261)에 대한 서양산 고추냉이 뿌리 추출물의 항균효과를 알아보고, 이를 대표적 항균제인 클로르헥시딘의 항균활성과 비교하여 다음과 같은 결과를 얻었다. 1. 서양산 고추냉이 뿌리 추출물은 연구에 사용된 7종의 구강내 미생물에 대해 최소억제농도는 30-125ppm, 최소살균농도는 125-500ppm을 나타냈으며, 특히 세균보다는 효모인 C. albicans에 대해 강한 항균활성을 나타낸다. 2. 클로르헥시딘 역시 7종의 구강내 미생물에 대해 항균효과를 나타냈으며, 최소억제농도는 0.15-2.5%, 최소살균농도는 0.4-2.5%로 측정되었다. 이상의 결과로 서양산 고추냉이 뿌리 추출물은 클로르헥시딘과 비교하여 더 낮은 농도에서 클로르헥시딘과 유사한 정도의 항균효과를 나타냄을 알 수 있다.

Abstract

In this study, the antimicrobial effects of Horseradish( Armoracia rusticana) root extracts against oral pathogens were investigated, and also compared with that of chlorhexidine. The following 7 microorganisms were used in this study: Streptococcus mutans ATCC 25175, Streptococcus sobrinus(d) ATCC 27607, Lactobacillus casei ATCC 393, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Actinobacillus actinomycetemcomitans ATCC 29522, Candida albicans ATCC 10261. Horseradish root extracts and chlorhexidine were tested to determine their minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC). The results of this study can be summarized as follows: 1. Horseradish root extracts showed antimicrobial effect against the tested oral pathogens. MIC and MBC of this extracts were 30-125, 125-500ppm, respectively. Especially, it was the most effective against C. albicans of other tested microorganisms. 2. Chlorhexidine also showed antimicrobial effect against the tested oral pathogens. MIC of chlorhexidine range between 0.15 and 2.5%, MBC are 0.4-2.5%. In conclusion, it was suggested that AIT had similar antimicrobial effects in the lower concentration, compared with that of chlorhexidine.

참고문헌 (45)

  1. "Dental plaque formation", 2, pp.1599~1607 (2000)
  2. "Longitudinal investigation of the role of Streptococcus mutans in human fissure decay", 26, pp.498~507 (1979)
  3. "The oral microflora and biofilms on teeth", pp.29~48 (2003), Blackwell Munksgaard
  4. "The predominant cultivable microflora of advanced periodontitis", 85, pp.114~121 (1977)
  5. "The occurrence of Actinobacillus actinomycetemcomitans,Bacteroides gingivalis,and Bacteroides intermedius in destructive periodontal disease in adults", 13, pp.570~577 (1986)
  6. "Bacteroides gingivalis,Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases", 15, pp.85~93 (1988)
  7. "Clinical Periodontology and Implant Dentistry, 4th ed.", pp.218~225 (2003), Blackwell Munksgaard
  8. "Antimicrobial effect of chlorhexidine in a controlled release delivery system", 1, pp.33~36 (1990)
  9. "Some antibacterial properties of chlorhexidine", 8, pp.61~67 (1973)
  10. "Chlorhexidine as a denture disinfectant in the treatment of denture stomatitis", 80, pp.457~464 (1972)
  11. "The mode of action of chlorhexidine", 8, pp.68~75 (1973)
  12. "Chlorhexidine in dental practice", 1, pp.143~152 (1974)
  13. "The role of antimicrobials", pp.179~188 (2003), Blackwell Munksgaard
  14. "The effect of chlorhexidine gluconate irrigation on the canal flora of freshly extracted necrotic teeth", 53, pp.518~522 (1988)
  15. "Effectiveness of 2% chlorhexidine gel and calcium hydroxide against Enterococcus faecalis in bovine root dentine in vitro", 36, pp.267~275 (2003)
  16. "Intracanal medicaments: evaluation of the antibacterial effects of chlorhexidine, metronidazole, and calcium hydroxide associated with three vehicles", 49, pp.167~169 (1997)
  17. "Disturbances of taste from oral disinfectants", 26, pp.335~341 (1978)
  18. "Analysis of volatile components in essential oil of upland wasabi and their inhibitory effects on platelet aggregation", pp.2131~2135 (1994)
  19. "Volatile components of wasabi", pp.365~370 (1981), Cocholeria armoracia
  20. "Glucosinolates and their breakdown products in food and food plants", 18, pp.123~201 (1983)
  21. "A review of mechanisms underlying anticarcinogenicity by brassica vegetables", 103, pp.79~129 (1997)
  22. "Studies on the pungent component.Antibacterial properties of essential oil of Eutrema wasabi Maxim", 78, pp.435~443 (1958)
  23. "Inhibitory effect of volatile constituents of plants on the proliferation of bacteria-Antibacterial activity of plant volatiles", 11, pp.609~615 (1983)
  24. "Comparison of effects of Wasabia japonica and allyl isothiocyanate on the growth of four strains of Vibrio parahaemolyticus in lean and fatty tuna meat suspensions", 49, pp.27~34 (1999)
  25. "Study on antimicrobial and antimutagenic activity of horseradish( Wasabia japonica) root extracts.", 31, pp.835~841 (1998)
  26. "Bactericidal activity of wasabi(Wasabia japonica)against Helicobacter pylori", 94, pp.255~261 (2004)
  27. "Associations of endodontic symptoms and signs with particular combinations of specific bacteria", 29, pp.69~75 (1996)
  28. "Evaluation of an assay method of the susceptibility of antimicrobial agents using a 96-well flat-bottom microplate and a microplate reader", 92, pp.659~664 (1997)
  29. "Preliminary examination of allyl isothiocyanate vapor for food preservation", pp.1476~1477 (1992)
  30. "Interaction of oxidized glutathione with allyl isothiocyanate", 24, pp.715~718 (1985)
  31. "Interaction of proteins with allyl isothiocyanate", 35, pp.85~88 (1987)
  32. "Studies on the effect of isothiocyanates and their analogues on microorganisms effects of isothiocyanates on the oxygen uptake of yeasts", pp.740~746 (1971)
  33. "Flavor compound and storage stability of essential oil from garlic distillation", 22, pp.840~845 (1990)
  34. "Essential oils of Thymus quinquecostatus Celakov.and Tymus magus Nakai", 2, pp.234~240 (1994)
  35. "Allyl isothiocyanate content in different plant parts of Wasabia japonica Mastum", 42, pp.281~285 (1997)
  36. "Antimicrobial effects of allyl isothiocyanates on several microorganisms", 31, pp.206~211 (1999)
  37. "Effect of caries preventive measures in children highly infected with the bacterium of Streptococcus mutans", 27, pp.861~868 (1982)
  38. "In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite", 97, pp.79~84 (2004)
  39. "The effect of mouthrinses and topical application of chlorhexidine on the development of dental plaque nd gingivitis in man", 5, pp.79~83 (1970)
  40. "Effect of leafextract from Camellia sinensis and seed-extract from Casia tora on viability of Mutans Streptococci isolated from the interface between orthodontic brackets and tooth surfaces", 33, pp.381~389 (2003)
  41. "Antimicrobial effects of essential oils in combination with chlorhexidine digluconate", 20, pp.221~225 (2005)
  42. "Antimicrobial effects of tea tree oil(Melaleuca alternifolia)on oral microorganisms", 110, pp.125~130 (2000)
  43. "Efficacy of sodium hypochlorite and coconut soap used as disinfecting agents in the reduction of denture stomatitis,Streptococcus mutans and Candida albicans", 31, pp.453~459 (2004)
  44. "A comparison of the antibacterial efficacies of essential oils against oral pathogens", 19, pp.61~54 (2004)
  45. "In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens", 45, pp.141~148 (2000)

관련 논문AI추천

구강 내 미생물에 대한 서양산 고추냉이(Horseradish, Armoracia rusticana) 뿌리 천연추출물과 합성 Allyl isothiocyanate의 항균활성 비교박호원, 백광선, 서현우, 신일식, 이주현대한소아치과학회 · 대한소아치과학회지2009 · 제36권 제2호구강에서 채취한 혐기성 세균에 대한 서양산 고추냉이(Horseradish, Armoracia rusticana) 뿌리 추출물의 항균효과박호원, 서현우, 신일식, 이주현, 장용걸대한소아치과학회 · 대한소아치과학회지2010 · 제37권 제2호치태에서 분리된 Streptococcus mutans에 대한 서양산 고추냉이(Armoracia rusticana) 뿌리 추출물의 항균효과김혜경, 박호원, 서현우, 신일식, 이주현대한소아치과학회 · 대한소아치과학회지2008 · 제35권 제2호치근관 내 편성 혐기성 세균에 대한 서양산 고추냉이 뿌리 추출물의 항균효과박호원, 서현우, 신일식, 이원주, 이주현대한소아치과학회 · 대한소아치과학회지2009 · 제36권 제2호근관세척제로써 서양산 고추냉이(Horseradish, Armoracia rusticana) 뿌리 추출물의 항균효과박호원, 서현우, 윤효진, 이주현대한소아치과학회 · 대한소아치과학회지2011 · 제38권 제2호서양산 고추냉이 추출물이 함유된 세치제의 효능에 대한 임상적 실험연구김종수, 박호원, 서현우, 신일식, 이시영대한소아치과학회 · 대한소아치과학회지2012 · 제39권 제1호

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