OrthoMTA와 ProRoot MTA의 항미생물 효과 비교
COMPARISON OF ANTIMICROBIAL EFFECTS OF ORTHOMTA AND PROROOT MTA
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초록
본 연구의 목적은 현재 많이 사용되고 있는 ProRoot MTA (Dentsply Tulsa, USA)와 최근 국내에서 새로 개발된OrthoMTA (Bio MTA, Korea)의 감염된 근관에서 주로 발견되는 3개의 미생물에 대한 효과를 다양한 농도와 시간에서 비교하는 것이다. 50, 25, 12.5 mg/mL의 농도로 준비된 ProRoot MTA와 OrthoMTA에 Enterococcus faecalis,Fusobacterium nucleatum 그리고 Staphylococcus epidermidis를 접종 후 24, 48, 72시간에 항미생물 효과를 관찰하여다음과 같은 연구결과를 얻었다. E. faecalis의 경우 ProRoot MTA와 OrthoMTA 50, 25, 12.5 mg/mL 농도의 모든 시간대에서 세균을 완전히 억제하지는 못했지만 양성 대조군에 비해 성장이 조금 감소한 양상을 보였다(p < 0.05). F. nucleatum의 경우 ProRoot MTA 50mg/mL 농도의 24시간에서 미생물을 억제하였으나 시간이 지남에 따라 세균의 성장을 보인 반면 OrthoMTA의 경우 같은농도에서 시간이 지나도 계속 세균을 억제하였다(p < 0.05). S. epidermidis의 경우 ProRoot MTA 50 mg/mL,OrthoMTA 50, 25 mg/mL 농도의 24시간에서 억제 효과를 보였으나 시간이 지나면서 세균이 조금씩 성장하였다. 그러나전체적으로 모든 농도와 시간에서 양성 대조군에 비해서는 억제 효과를 나타냈다(p < 0.05). 본 연구를 통해 국내에서 새로개발된 OrthoMTA는 기존의 ProRoot MTA보다 항균활성을 가지고 있음을 알 수 있었다., 2광주 보건대학교 치위생학교실
Abstract
The aim of this study was to compare two commercial root canal medicaments, ProRoot MTA (Dentsply Tulsa, USA) and OrthoMTA (Bio MTA, Korea), by assessing the antimicrobial effects on three selected species commonly found in root canals of infected teeth, namely Enterococcus faecalis, Fusobacterium nucleatum and Staphylococcus epidermidis. Colonies of these bacteria were treated with varied concentrations of ProRoot MTA or OrthoMTA over different lengths of time. The results are as follows :50, 25 and 12.5 mg/mL of ProRoot MTA or OrthoMTA did not completely inhibit the growth of E. faecalis,but a decreased growth rate was evident in comparison to the control (p < 0.05). 50 mg/mL of both materials successfully eliminated F. nucleatum during the first 24 hours. Regrowth of microbes after 24 hours, however,indicated a diminished effect of ProRoot MTA whereas OrthoMTA showed its continuously sustained antimicrobial actions (p < 0.05). 50 mg/mL of ProRoot MTA and 50, 25 mg/mL of OrthoMTA exerted their full antibacterial actions against S. epidermidis during the first 24 hours. Although the regrowth of colony was observed after 24 hours, the rate of growth was significantly decreased, approximating a complete inhibition (p < 0.05). The present study revealed that OrthoMTA, recently developed in Korea, had antimicrobial activity higher than ProRoot MTA.
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