과잉치 분류에 따른 치수유래줄기세포 계대 배양 시간의 연관성
Relationship with Passage Time of Human Dental Pulp Stem Cells from Supernumerary Tooth by Classification
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초록
본 연구는 상악 매복 과잉치를 가지고 전신질환 및 의과 병력이 없는 만 5 - 9세 사이의 남녀 20명으로부터 서면동의를 얻고 상악 전치부에 매복된 과잉치를 발치하고 치수세포를 채취하였다. 채취 후 세포의 1차 배양 동안 오염된 3명(남자 2명, 여자 1명)을 제외하고 총 17명(남자 10명, 여자 7명)을 대상으로 하였다. 이번 연구에서 과잉치 치수조직으로부터 유래한세포의 계대 배양에 소요된 평균 시간은 2.91 ± 0.29일이었다. 최초 치수조직으로부터 세포를 얻은 후 80% confluency를얻는데 소요되는 시간이 4.53 ± 0.94일로 가장 많이 걸렸고, 이후 계대부터는 2.73 ± 0.32일이 소요되었다. 남자의 비율은 58.82%, 여자의 비율은 41.18%이었다. 여자의 평균 소요시간은 2.81 ± 0.27일 이었으며, 남자는 2.98 ± 0.29이었다. 과잉치가 역위된 비율은 82.35%, 정위 비율은 17.65%였다. 평균 계대 소요 배양 시간은 역위가 2.94 ± 0.30일, 정위는2.80 ± 0.20일이었다. 원뿔형(Conical type)은 76.47%였고, 그 외 형태는 23.53%였다. 원뿔형의 평균 소요시간은 2.92 ± 0.31이었고, 그 외 형태의 소요 시간은 2.88 ± 0.22이었다. 매복 방향과 형태에 따른 계대 배향 시간은 통계적으로 유의한 차이를 보이지 않았다. 향후 초기 계대와 후기 계대에서 얻어진 세포의 줄기세포로써의 능력을 평가하는 후속 연구들이 필요하며, 3일 이내의 계대 배양 시간이 소요된 점을 고려할 때, 연구의 효율성과 빠른 배양시간 등은 과잉치 유래세포가 치아유래 줄기세포의 연구에 활용가능성이 충분하리라 판단되었다.
Abstract
For this research 20 supernumerary teeth impacted in the maxillary anterior have been extracted and pulp cells have been collected from them. From the collected pulp cells, total of 17 (10 males, 7 females) have been selected as subjects. From this research, the run-time of successive culture of the cell from tooth number pulp tissue was 2.91 ± 0.29 days. From the gathering of cells from the initial pulp tissue until gaining 80% confluency took 4.53 ± 0.94 which was the longest. The following successive cultures took 2.73 ± 0.32 days. Average runtime for female was 2.81 ± 0.27 days whereas male had average runtime of 2.98 ± 0.29 days. Average run-time for inversion was 2.94 ± 0.30 days and for normal location, 2.80 ± 0.20 days. Average runtime was 2.92 ± 0.31 days and other forms took 2.88 ± 0.22 days. In the future, follow up research would be needed to evaluate the efficiency of the cells collected from the initial passage and the latter passage as stem-cells and taking into consideration the less than 3 days’time for the subculture, it could be concluded that the research efficiency and fast cultivation would be sufficiently effective.
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