Susceptibility of Mutans streptococci in the Planktonic and Biofilm State to Erythrosine
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The aim of this study was to investigate the susceptibility of Mutans streptococci in both planktonic and biofilm states to erythrosine. S. mutans was cultured in brain-heart infusion (BHI) broth. Erythrosine was diluted in BHI broth and prepared at a concentration range of 0.02 - 10000 μg/L. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured using the microdilution method. After forming biofilms on 96-well plates, the minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were measured. S. mutans was susceptible to erythrosine in both planktonic and biofilm states. MIC and MBC values were both 19.5 μg/L for the planktonic state, while MBIC and MBEC values were 313 μg/L and 2500 μg/L, respectively, for the biofilm state. Erythrosine (19.5 μg/L) exhibited a bactericidal effect on S. mutans (killing 99.9%) in the planktonic state. For biofilms, erythrosine inhibited biofilm growth and eradicated 99.9% of biofilm bacteria at higher concentrations than MIC and MBC. These MBIC and MBEC concentrations are much lower than known noxious doses, and the MIC, MBC, and MBIC values were even lower than clinical concentrations.
Abstract
The aim of this study was to investigate the susceptibility of Mutans streptococci in both planktonic and biofilm states to erythrosine. S. mutans was cultured in brain-heart infusion (BHI) broth. Erythrosine was diluted in BHI broth and prepared at a concentration range of 0.02 - 10000 μg/L. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured using the microdilution method. After forming biofilms on 96-well plates, the minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were measured. S. mutans was susceptible to erythrosine in both planktonic and biofilm states. MIC and MBC values were both 19.5 μg/L for the planktonic state, while MBIC and MBEC values were 313 μg/L and 2500 μg/L, respectively, for the biofilm state. Erythrosine (19.5 μg/L) exhibited a bactericidal effect on S. mutans (killing 99.9%) in the planktonic state. For biofilms, erythrosine inhibited biofilm growth and eradicated 99.9% of biofilm bacteria at higher concentrations than MIC and MBC. These MBIC and MBEC concentrations are much lower than known noxious doses, and the MIC, MBC, and MBIC values were even lower than clinical concentrations.
참고문헌 (10)
- Skinner FH, "The prevention of pyorrhea and dental caries by prophylaxis", Dent Cosmos, 56, pp.299~309 (1914)
- "Chemicals &Laboratory Equipment"[URL]
- Begue WJ, "Microbial inhibition by erythrosine", J Dent Res, 45, pp.1464~1467 (1966)
- Wood S, "Erythrosine is potential photosensitizer for the photodynamic therapy of oral plaque biofilms", J Antimicrob Chemother, 57, pp.680~684 (2006)
- Baab DA, "A comparison of antimicrobial activity of four disclosant dyes", J Dent Res, 62, pp.837~841 (1983)
- Stewart PS, "Antibiotic resistance of bacteria in biofilms", Lancet, 358, pp.135~138 (2001)
- Ceri H, "The calgary biofilm device : new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms", J Clin Microbiol, 37, pp.1771~1776 (1999)
- Reiter KC, "Inhibition of biofilm maturation by linezolid in meticillin-resistant Staphylococcus epidermidis clinical isolates : comparison with other drugs", J Med Microbiol, 62, pp.394~399 (2013)
- Sandoe JA, "Enterococcal intravascular catheter-related bloodstream infection : management and outcome of 61 consecutive cases", J Antimicrob Chemother, 50, pp.577~582 (2002)
- Caldwell RC, "The effect of a dextranase mouthwash on dental plaque in young adults and children", J Am Dent Assoc, 82, pp.124~131 (1971)