Potential of Erythrosine-Mediated Photodynamic Therapy as a Cavity Disinfectant: Antibacterial Efficacy and Bonding Ability
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This study aimed to evaluate the antibacterial efficacy of erythrosine-mediated photodynamic therapy (PDT) against Streptococcus mutans (S. mutans ) in dentin and its effect on the shear bond strength (SBS) of composite resin to dentin. Eighty extracted human noncarious premolars were used in this study. Forty teeth were used for the antibacterial activity test, while the remaining 40 were used for the SBS test. Both experiments were conducted with 4 experimental groups (n = 10): control (distilled water), sodium hypochlorite (NaOCl, 6%), chlorhexidine (CHX, 0.12%), and erythrosine-mediated PDT. Antibacterial effects were evaluated by counting S. mutans colony-forming units (CFUs). The SBS of composite resins to dentin was measured using a universal testing machine. All treatments (NaOCl, CHX, and PDT) demonstrated statistically significant differences in antibacterial activity compared with the control group (p < 0.05). The antibacterial effects were ranked from strongest to weakest as follows: NaOCl, PDT, and CHX. In the SBS test, the NaOCl group exhibited a statistically significant difference compared with the CHX, PDT, and control groups (p < 0.05), with the lowest bond strength. No statistically significant differences were found among the CHX, PDT, and control groups (p > 0.05). Erythrosine-mediated PDT exhibited significant antibacterial effects against S. mutans , with higher antibacterial activity than CHX but lower than NaOCl. Only NaOCl negatively affected the bond strength of composite resin to dentin. In conclusion, erythrosine-mediated PDT shows potential as a cavity disinfectant due to its significant antibacterial effects against S. mutans and lack of adverse effects on bond strength.
Abstract
This study aimed to evaluate the antibacterial efficacy of erythrosine-mediated photodynamic therapy (PDT) against Streptococcus mutans (S. mutans ) in dentin and its effect on the shear bond strength (SBS) of composite resin to dentin. Eighty extracted human noncarious premolars were used in this study. Forty teeth were used for the antibacterial activity test, while the remaining 40 were used for the SBS test. Both experiments were conducted with 4 experimental groups (n = 10): control (distilled water), sodium hypochlorite (NaOCl, 6%), chlorhexidine (CHX, 0.12%), and erythrosine-mediated PDT. Antibacterial effects were evaluated by counting S. mutans colony-forming units (CFUs). The SBS of composite resins to dentin was measured using a universal testing machine. All treatments (NaOCl, CHX, and PDT) demonstrated statistically significant differences in antibacterial activity compared with the control group (p < 0.05). The antibacterial effects were ranked from strongest to weakest as follows: NaOCl, PDT, and CHX. In the SBS test, the NaOCl group exhibited a statistically significant difference compared with the CHX, PDT, and control groups (p < 0.05), with the lowest bond strength. No statistically significant differences were found among the CHX, PDT, and control groups (p > 0.05). Erythrosine-mediated PDT exhibited significant antibacterial effects against S. mutans , with higher antibacterial activity than CHX but lower than NaOCl. Only NaOCl negatively affected the bond strength of composite resin to dentin. In conclusion, erythrosine-mediated PDT shows potential as a cavity disinfectant due to its significant antibacterial effects against S. mutans and lack of adverse effects on bond strength.
참고문헌 (47)
- GBD 2016 Disease, "Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990-2016 : a systematic analysis for the Global Burden of Disease Study 2016", Lancet, 390, pp.1211~1259 (2017)
- Boston DW, "Histological study of an acid red caries-disclosing dye", Oper Dent, 14, pp.186~192 (1989)
- Brännström M, "The cause of postoperative sensitivity and its prevention", J Endod, 12, pp.475~481 (1986)
- Imazato S, "Bactericidal effect of dentin primer containing antibacterial monomer methacryloyloxydodecylpyridinium bromide(MDPB)against bacteria in human carious dentin", J Oral Rehabil, 28, pp.314~319 (2001)
- Hashem AA, "The effect of different irrigating solutions on bond strength of two root canal-filling systems", J Endod, 35, pp.537~540 (2009)
- Haapasalo M, "Irrigation in endodontics", Dent Clin North Am, 54, pp.291~312 (2010)
- Costa ACBP, "Susceptibility of Candida albicans and Candida dubliniensis to erythrosine-and LED-mediated photodynamic therapy", Arch Oral Biol, 56, pp.1299~1305 (2011)
- Campanholi KSS, "Biomedical platform development of a chlorophyll-based extract for topic photodynamic therapy : mechanical and spectroscopic properties", Langmuir, 34, pp.8230~8244 (2018)
- Lee YH, "The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit", Int J Oral Sci, 4, pp.196~201 (2012)
- Pereira CA, "Photodynamic inactivation of Streptococcus mutans and Streptococcus sanguinis biofilms in vitro", Lasers Med Sci, 28, pp.859~864 (2013)
- Wood S, "Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms", J Antimicrob Chemother, 57, pp.680~684 (2006)
- Alkhudhairy F, "Adhesive bond integrity of dentin conditioned by photobiomodulation and bonded to bioactive restorative material", Photodiagnosis Photodyn Ther, 28, pp.110~113 (2019)
- Ferracane JL, "Resin composite-state of the art", Dent Mater, 27, pp.29~38 (2011)
- Türkün M, "Effect of cavity disinfectants on the sealing ability of nonrinsing dentin-bonding resins", Quintessence Int, 35, pp.469~476 (2004)
- Meiers JC, "Cavity disinfectants and dentin bonding", Oper Dent, 21, pp.153~159 (1996)
- Perdigao J, "Effects of chlorhexidine on dentin surfaces and shear bond strengths", Am J Dent, 7, pp.81~84 (1994)
- Cao DS, "Effect of tooth disinfecting procedures on dentin shear bond strength", J Dent Res, 74, pp.73~ (1995)
- Vianna ME, "In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 97, pp.79~84 (2004)
- McDonnell G, "Antiseptics and disinfectants : activity, action, and resistance", Clin Microbiol Rev, 12, pp.147~179 (1999)
- Arias-Moliz MT, "Enterococcus faecalis biofilms eradication by root canal irrigants", J Endod, 35, pp.711~714 (2009)
- Chiniforush N, "Clinical approach of high technology techniques for control and elimination of endodontic microbiota", J Lasers Med Sci, 6, pp.139~150 (2015)
- Castano AP, "Mechanisms in photodynamic therapy : Partone-Photosensitizers, photochemistry and cellular localization", Photodiagnosis Photodyn Ther, 1, pp.279~293 (2004)
- Vohra F, "Role of antimicrobial photodynamic therapy in the treatment of aggressive periodontitis : a systematic review", Photodiagnosis Photodyn Ther, 13, pp.139~147 (2016)
- Hamblin MR, "Oxygen-Independent Antimicrobial Photoinactivation : Type III Photochemical Mechanism?", Antibiotics, 9, pp.53~ (2020)
- Araújo NC, "Photodynamic effects of curcumin against cariogenic pathogens", Photomed Laser Surg, 30, pp.393~399 (2012)
- Jurič IB, "The antimicrobial effectiveness of photodynamic therapy used as an addition to the conventional endodontic re-treatment : a clinical study", Photodiagnosis Photodyn Ther, 11, pp.549~555 (2014)
- Metcalf D, "Enhancement of erythrosine-mediated photodynamic therapy of Streptococcus mutans biofilms by light fractionation", J Antimicrob Chemother, 58, pp.190~192 (2006)
- Choi S, "Optimum treatment parameters for photodynamic antimicrobial chemotherapy on Streptococcus mutans biofilms", J Korean Acad Pediatr Dent, 42, pp.151~157 (2015)
- Baab DA, "A comparison of antimicrobial activity of four disclosant dyes", J Dent Res, 62, pp.837~841 (1983)
- Marshall GW Jr, "Sodium hypochlorite alterations of dentin and dentin collagen", Surf Sci, 491, pp.444~455 (2001)
- Toledano M, "Effect of acid etching and collagen removal on dentin wettability and roughness", J Biomed Mater Res, 47, pp.198~203 (1999)
- Hugo WB, "The effect of chlorhexidine on the electrophoretic mobility, cytoplasmic constituents, dehydrogenase activity and cell walls of Escherichia coli and Staphylococcus aureus", J Pharm Pharmacol, 18, pp.569~578 (1966)
- Perdigão J, "An ultra-morphological characterization of collagen-depleted etched dentin", Am J Dent, 12, pp.250~255 (1999)
- Wakabayashi Y, "Effect of dissolution of collagen in adhesion to dentin", Int J Prosthodont, 7, pp.302~306 (1994)
- Arias VG, "Effects of sodium hypochlorite gel and sodium hypochlorite solution on dentin bond strength", J Biomed Mater Res B Appl Biomater, 72, pp.339~344 (2005)
- Uno S, "Function of the hybrid zone as a stress-absorbing layer in resin-dentin bonding", Quintessence Int, 26, pp.733~738 (1995)
- Ozturk B, "Effect of NaOCl on bond strengths of bonding agents to pulp chamber lateral walls", J Endod, 30, pp.362~365 (2004)
- Nikaido T, "Bond strengths to endodontically-treated teeth", Am J Dent, 12, pp.177~180 (1999)
- Hawkins CL, "Hygochlorite-induced oxidation of proteins in plasma : Formation of chloramines and nitrogen-centred radicals and their role in protein fragmentation", Biochem J, 340, pp.539~548 (1999)
- Campos EA, "Influence of chlorhexidine concentration on microtensile bond strength of contemporary adhesive systems", Braz Oral Res, 23, pp.340~345 (2009)
- Di Hipólito V, "Effectiveness of self-adhesive luting cements in bonding to chlorhexidine-treated dentin", Dent Mater, 28, pp.495~501 (2012)
- Castro FLA, "Effect of 2% chlorhexidine on microtensile bond strength of composite to dentin", J Adhes Dent, 5, pp.129~138 (2003)
- Soares CJ, "Effect of chlorhexidine application on microtensile bond strength to dentin", Oper Dent, 33, pp.183~188 (2008)
- Chang YE, "Effect of chlorhexidine application methods on microtensile bond strength to dentin in Class I cavities", Oper Dent, 35, pp.618~623 (2010)
- Hassan AM, "The effect of different disinfecting agents on bond strength of resin composites", Int J Dent, 2014, pp.231235~ (2014)
- Hebling J, "Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo", J Dent Res, 84, pp.741~746 (2005)
- Demarco FF, "Dyes for caries detection influence sound dentin bond strength", Oper Dent, 23, pp.294~298 (1998)
