백합나무(Liriodendron tulipifera) 옥살산 전처리에 의해 생성된 액상가수분해산물의 에탄올 발효에 영향을 미치는 요인 분석
Analysis of Factors Affecting Ethanol Fermentation of Hydrolysate Derived from the Oxalic Acid Pretreatment of Yellow Poplar (Liriodendron tulipifera)
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
This study examined the optimal conditions for the oxalic acid pretreatment of yellow poplar for ethanol production depending on the combined severity factor (CSF). The factors affecting the ethanol fermentation of the hydrolysate were also analyzed. Xylose was the most abundant compound (2.80-16.44 g/L) in the hydrolysate, whereas the concentration of glucose was relatively low at 1.65-3.69 g/L. Ethanol production was highest at CSF 1.22 (10 min, 41 mM) after fermentation for 96 h, which resulted in a theoretical yield of 74.51% and an ethanol productivity of 0.027 g/L·h. The concentration of fermentation inhibitors (acetic acid, furfural, and total phenolic compounds) increased with increasing CSF. Ethanol production decreased significantly with increasing concentration of fermentation inhibitors at CSF higher than 1.59. The xylo-oligomer concentration was highest (18.77 g/L) at CSF 0.72, and the concentration decreased at CSF 1.52. The average molecular weights of the hydrolysate decreased slightly with increasing CSF. The degradation products contained in the hydrolysate were detected by gas chromatography-mass spectrometry. Among these products, the concentration of vanillin and syringaldehyde increased rapidly at high CSF.
Abstract
This study examined the optimal conditions for the oxalic acid pretreatment of yellow poplar for ethanol production depending on the combined severity factor (CSF). The factors affecting the ethanol fermentation of the hydrolysate were also analyzed. Xylose was the most abundant compound (2.80-16.44 g/L) in the hydrolysate, whereas the concentration of glucose was relatively low at 1.65-3.69 g/L. Ethanol production was highest at CSF 1.22 (10 min, 41 mM) after fermentation for 96 h, which resulted in a theoretical yield of 74.51% and an ethanol productivity of 0.027 g/L·h. The concentration of fermentation inhibitors (acetic acid, furfural, and total phenolic compounds) increased with increasing CSF. Ethanol production decreased significantly with increasing concentration of fermentation inhibitors at CSF higher than 1.59. The xylo-oligomer concentration was highest (18.77 g/L) at CSF 0.72, and the concentration decreased at CSF 1.52. The average molecular weights of the hydrolysate decreased slightly with increasing CSF. The degradation products contained in the hydrolysate were detected by gas chromatography-mass spectrometry. Among these products, the concentration of vanillin and syringaldehyde increased rapidly at high CSF.
참고문헌 (34)
- Yu, J., "Process integration for ethanol production from corn and corn stover as mixed substrates", Bioresour. Technol, 279, pp.10~16 (2019)
- Liu, L., "Simultaneous saccharification and co-fermentation of corn stover pretreated by H2O2 oxidative degradation for ethanol production", Energy, 168, pp.946~952 (2019)
- Mikulski, D., "Efficiency of dilute sulfuric acid pretreatment of distillery stillage in the production of cellulosic ethanol", Bioresour. Technol, 268, pp.424~433 (2018)
- Jeong, S.Y., "Optimization of pretreatment condition for ethanol production from oxalic acid pretreated biomass by response surface methodology", Ind. Crops and Prod, 79, pp.1~6 (2016)
- Cl from a hydrolysate of lignocellulosic biomass using electrodialysis, "Sep. Purif. Technol", 2013, 86~91
- Lee, H.J., "A feasibility study on the multi stage process for the oxalic acid pretreatment of a lignocellulosic biomass using electrodialysis", Bioresour. Technol, 130, pp.211~217 (2013)
- Mikulski, D., "Microwave-assisted pretreatment of maize distillery stillage with the use of dilute sulfuric acid in the production of cellulosic ethanol", Bioresour. Technol, 278, pp.318~328 (2019)
- Toscan, A., "New two-stage pretreatment for the fractionation of lignocellulosic components using hydrothermal pretreatment followed by imidazole delignification: Focus on the polysaccharide valorization", Bioresour. Technol, 285, pp.121346~121355 (2019)
- Annual report on forest and forestry trends, "Korea Forest Service", 1~465 (2017)
- Kim, H.Y., "Response surface optimization ofoxalic acid pretreatment of yellow poplar (Liriodendron tulipifera) forproduction of glucose and xylose monosaccharides", Bioresour. Technol, 102(2), pp.1440~1446 (2011)
- Jeong, S.Y., "Sequential Fenton oxidation and hydrothermal treatment to improve the effect of pretreatment and enzymatic hydrolysis on mixed hardwood", Bioresour. Technol, 200, pp.121~127 (2016)
- Pedersen, M., "Low temperature lignocellulose pretreatment: effects and interactions of pretreatment pH are critical for maximizing enzymatic monosaccharide yields from wheat straw", Biotechnol. Biofuels, 4, pp.1~10 (2010)
- Lim, W.S., "Effects of pretreatment factors on fermentable sugar production and enzymatic hydrolysis of mixed hardwood", Bioresour. Technol, 130, pp.97~101 (2013)
- Abatzoglou, N., "Phenomenological kinetics of complex systems: the development of a generalized severity parameter and its application to lignocellulosics fractionation", Chem. Eng. Sci, 47, pp.1109~1122 (1992)
- Scordia, D., "Effectiveness of dilute oxalic acidpretreatment of Miscanthus × giganteusbiomass for ethanol production", Biomass and Bioenergy, 59, pp.540~548 (2013)
- Singleton, V.L., "Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent", Methods in Enzymology, 299, pp.152~178 (1999)
- Determination of structural carbohydrates and lignin in biomass. NREL Laboratory Analytical Procedures, "Sluiter, A.", National Renewable Energy Laboratory, ~ (2004)
- Wang, X, "Efficient catalytic conversion of dilute-oxalic acid pretreated bagasse hydrolysate to furfural using recyclable ironic phosphates catalysts", Bioresour. Technol, 290, pp.121764~121771 (2019)
- Jeong, S.Y., "Improvement of the fermentability of oxalic acid hydrolysates by dextoxification using electrodialysis and adsorption", Bioresour. Technol, 152, pp.444~449 (2014)
- Girio, F.M., "Hemicelluloses for fuel ethanol: a review", Bioresour. Technol, 101(13), pp.4775~4800 (2010)
- Larsson, S., "The generation of fermentation inhibitors during dilute acid hydrolysis of softwood", Enzyme Microb. Technol, 24(3-4), pp.151~159 (1999)
- Yu, Z., "Enhancing fermentable sugar yield from cassava residue using a two-step dilute ultra-low acid pretreatment process", Ind. Crops and Prod, 124, pp.555~562 (2018)
- Lee, J.W., "Efficiencies of acid catalysts in the hydrolysis of lignocellulosic biomass over a range of combined severity factors", Bioresour. Technol, 102(10), pp.5884~5890 (2011)
- 이윤주, "팜 부산물(Empty Fruit Bunch) 열수처리 액상가수분해산물의 재사용 평가", 신재생에너지, 15(2), pp.52~60 (2019), 한국신·재생에너지학회[DOI]
- Xue, S., "Water-soluble phenolic compounds produced from extractive ammonia pretreatment exerted binary inhibitory effects on yeast fermentation using synthetic hydrolysate", PLOS One, 13(3), pp.e0194012~ (2018)
- Delgenes, J., "Effects of lignocellulose degradation products on ethanol fermentations of glucose and xylose by Saccharomyces cerevisiae, Zymomonas mobilis, Pichia stipitis, and Candida shehatae", Enzyme Microb. Technol, 19(3), pp.220~225 (1996)
- Heipieper, H.J., "Mechanisms of resistance of whole cells to toxic organic solvents", Trends Biotechnol, 12(10), pp.409~415 (1994)
- Xiao, L.P., "Hydrothermal treatment and enzymatic hydrolysis of Tamarix ramosissima: Evaluation of the process as a conversion method in a biorefinery concept", Bioresour. Technol, 135, pp.73~81 (2013)
- Xue, S., "Sugar loss and enzyme inhibition due to oligosaccharide accumulation during high solids-loading enzymatic hydrolysis", Biotechnol. Biofuels, 8, pp.195~208 (2015)
- Bowman, M.J., "Liquid chromatography-mass spectrometry investigation of enzyme-resistant xylooligosaccharide structures of switchgrass associated with ammonia pretreatment, enzymatic saccharification, and fermentation", Bioresour. Technol, 110, pp.437~447 (2012)
- Ma, X.J., "Degradation and dissolution of hemicelluloses during bamboo hydrothermal pretreatment", Bioresour. Technol, 161, pp.215~220 (2014)
- Moniz, P., "Hydrothermal production and gel filtration purification of xylo-oligosaccharides from rice straw", Ind. Crops and Prod, 62, pp.460~465 (2014)
- Garrote, G., "Hydrothermal processing of lignocellulosic materials", Holz als Roh- und Werkstoff, 57(3), pp.191~202 (1999)
- Zhang, Y., "Effect of overliming and activated carbon detoxification on inhibitors removal and butanol fermentation of poplar prehydrolysates", Bioresour. Technol, 11, pp.178~191 (2018)