소양인 양격산화탕의 면역 활성화 연구
Stimulation of the Immune Response by Yanggyuksanhwa-tang
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초록
1. Objectives Yanggyuksanhwa-tang for Soyangin was applied to investigate the immunological activities on antigen (Ag)-specific or Ag-non-specific immune responses on murine macrophage cell line (RAW 264.7) and ovalbumin/aluminium (OVA/Alum)-immunized mice. 2. Methods This study were carried out in nitric oxide (NO) synthesis on RAW 264.7 cells and cellular proliferation on mouse splenocytes. C57BL/6 mice were immunized intraperitonially with OVA/Alum (100 μg/200 μg) on day 1, 8, and 15. Yanggyuksanhwa-tang was administrated to mice orally for 3 weeks from day 1. On day 22, OVA-, lipopolysaccharide (LPS)-, and concanavalin A (Con A)-stimulated splenocyte proliferation and antibodies (OVA-specific antibodies of the IgG, IgG1, and total IgM classes) in plasma were measured. 3. Results Yanggyuksanhwa-tang significantly enhanced cellular proliferation by LPS and Con A on splenocytes from OVA/Alum-immunized mice (p<.001). Yanggyuksanhwa-tang also significantly enhanced plasma OVA-specific IgG (p<.001), IgG1 (p<.001), and total IgM (p<.01) levels compared with the OVA/Alum group. 4. Conclusions These results suggested that Yanggyuksanhwa-tang for Soyangin could be used as immunopotent.
Abstract
1. Objectives Yanggyuksanhwa-tang for Soyangin was applied to investigate the immunological activities on antigen (Ag)-specific or Ag-non-specific immune responses on murine macrophage cell line (RAW 264.7) and ovalbumin/aluminium (OVA/Alum)-immunized mice. 2. Methods This study were carried out in nitric oxide (NO) synthesis on RAW 264.7 cells and cellular proliferation on mouse splenocytes. C57BL/6 mice were immunized intraperitonially with OVA/Alum (100 μg/200 μg) on day 1, 8, and 15. Yanggyuksanhwa-tang was administrated to mice orally for 3 weeks from day 1. On day 22, OVA-, lipopolysaccharide (LPS)-, and concanavalin A (Con A)-stimulated splenocyte proliferation and antibodies (OVA-specific antibodies of the IgG, IgG1, and total IgM classes) in plasma were measured. 3. Results Yanggyuksanhwa-tang significantly enhanced cellular proliferation by LPS and Con A on splenocytes from OVA/Alum-immunized mice (p<.001). Yanggyuksanhwa-tang also significantly enhanced plasma OVA-specific IgG (p<.001), IgG1 (p<.001), and total IgM (p<.01) levels compared with the OVA/Alum group. 4. Conclusions These results suggested that Yanggyuksanhwa-tang for Soyangin could be used as immunopotent.
참고문헌 (22)
- Lee JM, "Dongeuisusebowon", pp.243~271 (1992), Yeogang Publishing Co.
- Lee JM, "Dongeuisusebowon", pp.70~ (1964), Shinil Publishing Co.
- Kim KY, "The approach of Sasang constitutional medicine in incurable disease and immunity", J Sasang Constitut Med, 7(2), pp.113~128 (1995)
- 최애련, "凉膈散火湯으로 好轉된 少陽人外轉神經痲痺 斜視患者치험 1례", 사상체질의학회지, 20(3), pp.176~183 (2008), 사상체질의학회
- Lo HS, "The Effect of Yangkyuksanhwa-tang on cytokine production in the patients with cerebral infarction", J Sasang Constitut Med, 16(1), pp.120~129 (2004)
- 최동준, "급성기 중풍 환자에 대한 양격산화탕의 임상적 효능", 대한한의학회지, 25(1), pp.111~116 (2004), 대한한의학회
- 김상진, "중풍으로 유발된 배뇨？배변장애에 양격산화탕을 위졸 한 치험 1례", 대한본초학회지, 18(3), pp.1~8 (2003), 대한본초학회
- 정성일, "少陽人 당뇨병환자에 凉膈散火湯을 투여한 증례", 동의생리병리학회지, 16(6), pp.37~1313 (2002), 대한동의병리학회
- Cho SW, "Effects of Yangkyuksanhawtang on the allergic contact dermatitis", Korean Journal of Korean Medical Institute of Dermatology & Aesthetics, 1(1), pp.16~40 (2005)
- 소윤조, "간효소에 의해 대사된 양격산화탕의 저산소", 재관류로부터 PC12 세포 보호효과, 2005, pp.49(1), 97~102[DOI]
- Son SK, "Effects of Yanggyuksanhwatang on cerebral blood flow and Ischemic brain damage in rats", J Sasang Constitut Med, 13(2), pp.165~176 (2001)
- 부일권, "당뇨흰쥐의 국소뇌허혈에 대한 凉膈散火湯의 면역조직화학적 연구", 동의생리병리학회지, 21(3), pp.741~747 (2007), 대한동의병리학회
- Yun BH, "Yangkyuksanhwa-tang effected to atopic dermatitis", J Sasang Constitut Med, 16(2), pp.84~98 (2004)
- 김의정, "凉膈散火湯이 당뇨흰쥐의 전뇌허혈에 미치는 영향", 동의생리병리학회지, 22(2), pp.321~327 (2008), 대한동의병리학회
- 이승엽, "소양인 형방도적산과 양격산화탕의 항 스트레스효과에 관한 실험적 연구", 사상체질의학회지, 20(3), pp.151~163 (2008), 사상체질의학회
- Liew FY, "Tumor necrosis factor synergizes with IFN-γ in mediating killing of Leishmania major through the induction of nitric oxide", J Immunol, 145, pp.4306~4310 (1990)
- Thornberry NA, "Interleukin-1 beta converting enzyme: a novel cystein protease required for IL-1 beta production and implicated in progammed cell death", Protein Sci, 4, pp.3~12 (2005)
- Burger D, "Is IL-1 a good therapeutic target in the treatment of arthritis?", Best Pract Clin Rheumatol, 20(5), pp.879~896 (2006)
- Sung CS, "Cellular mechanisms of neuroinflammatory pain: the role of interleukin-1beta", Act Anaesthsiol Taiwan, 45(2), pp.103~109 (2007)
- Weiss L, "Cell and tissue biology 6th ed", pp.481~538 (1988), Uraban & Schwarzenbeg
- Zalys R, "In vivo effect of chronic treatment with (MET5)-enkephain on hematological values and natural killer cell activity in athymic mice", Life Sci, 66, pp.829~834 (2000)
- James GL, "Methods in Immunotoxicology", pp.2~15 (1995), John Wiley-Liss, Inc