고지방식이로 유도된 비만 생쥐모델에서 저령차전자탕의 항비만 효과
Anti-obesity Effect of Jeoreongchajeonja-tang in a High-fat Diet-induced Obesity Mouse model
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초록
Objective This study investigated the effects of Jeoreongchajeonja-tang in a high-fat diet-induced obesity mouse model. Methods The study examined 9-week-old male mice (C57bl/6J) divided into four groups: the normal(C57bl/6J-Nr), control (high-fat diet only; HFD-CTL), positive-control (high-fat diet with Garcinia cambogia), and experimental (high-fat diet with Jeoreongchajeonja-tang; HFD-JCT) groups. After 7 weeks, the body weight, food efficiency ratio, organ weight, and visceral fat weight of the mice were measured. Blood serum tests, mRNA, liver histopathology, and epididymis adipocytes were also examined. Results Compared with the Control(HFD-CTL) group, the Experimental(HFD-JCT) group given Jeoreongchajeonja-tang showed significant reductions in absolute body weight and food efficiency ratio. The serum alanine aminotransferase, total-cholesterol, triglyceride, low-density lipoprotein (LDL)-cholesterol, high-density lipoprotein (HDL)-cholesterol, insulin-like growth factor-1, and leptin levels were significantly lower in the experimental group than in the control group. The serum adiponectin levels were significantly higher in the experimental group than in the control group. Compared with the control group, the experimental group showed significant reductions in absolute abdominal subcutaneous fat, epididymal adipose tissue, kidney adipose tissue, intestine adipose tissue, and liver, kidney and spleen adipose tissue weights. The C/EBP-β, leptin, and SREBP1c/ADD1 mRNA expression were significantly lower in the experimental group than in the control group, while the UCP-2 and adiponectin mRNA expression were significantly higher. Compared with the control group, the experimental group showed a significant reduction in the absolute adipocyte area in the liver and epididymal adipose tissue. Conclusion Jeoreongchajeonja-tang has an anti-obesity effect. Additional clinical studies are expected.
Abstract
Objective This study investigated the effects of Jeoreongchajeonja-tang in a high-fat diet-induced obesity mouse model. Methods The study examined 9-week-old male mice (C57bl/6J) divided into four groups: the normal(C57bl/6J-Nr), control (high-fat diet only; HFD-CTL), positive-control (high-fat diet with Garcinia cambogia), and experimental (high-fat diet with Jeoreongchajeonja-tang; HFD-JCT) groups. After 7 weeks, the body weight, food efficiency ratio, organ weight, and visceral fat weight of the mice were measured. Blood serum tests, mRNA, liver histopathology, and epididymis adipocytes were also examined. Results Compared with the Control(HFD-CTL) group, the Experimental(HFD-JCT) group given Jeoreongchajeonja-tang showed significant reductions in absolute body weight and food efficiency ratio. The serum alanine aminotransferase, total-cholesterol, triglyceride, low-density lipoprotein (LDL)-cholesterol, high-density lipoprotein (HDL)-cholesterol, insulin-like growth factor-1, and leptin levels were significantly lower in the experimental group than in the control group. The serum adiponectin levels were significantly higher in the experimental group than in the control group. Compared with the control group, the experimental group showed significant reductions in absolute abdominal subcutaneous fat, epididymal adipose tissue, kidney adipose tissue, intestine adipose tissue, and liver, kidney and spleen adipose tissue weights. The C/EBP-β, leptin, and SREBP1c/ADD1 mRNA expression were significantly lower in the experimental group than in the control group, while the UCP-2 and adiponectin mRNA expression were significantly higher. Compared with the control group, the experimental group showed a significant reduction in the absolute adipocyte area in the liver and epididymal adipose tissue. Conclusion Jeoreongchajeonja-tang has an anti-obesity effect. Additional clinical studies are expected.
참고문헌 (40)
- Bray GA, "The Battle of the Bulge: A History of Obesity Research" (2007), Dorrance
- Guh DP, "The of co-morbidities related to obesity and overweight: a systematic and meta-analysis", BMC Public Health, 9, pp.88~ (2009)[DOI]
- Lenz M, "The morbidity and mortality associated with and obesity in adulthood: a systematic review", Dtsch Arztebl Int, 106(40), pp.641~648 (2009)
- Oh SW, "Effects of excess weight on cancer incidences depending on cancer sites and histologic findings among men: Korea National Health Insurance Corporation Study", J Clin Oncol, 23(21), pp.4742~4754 (2005)[DOI]
- Kang Jae hun, "Korean Obesity Treatment Guideline 2014", Korean Soceity For The Study Of Obesity, ~ (2014)
- Dennis L.Kasper, "Harrison's Principles Of Internal Medicine 19th edition" (2015), McGraw-Hill Education
- Lee, "The Reductive Effects of Oriental Medicine on the Body Fat and Abdominal Obesity", The Society of Korean Medicine for Obesity Research, 1(1), pp.33~42 (2001)
- Moon Soo Jung, "Review of Clinical Studies on Oriental Medicine Treatment for Obesity in the Korean Literature", Master, pp.2010
- Go Ga Yeon, "Anti-obesity effect of Yeoldahanso-tang on high fat diet-induced obesity mice", Doctoral, pp.2016
- Park Jae-Hyoung, "Effects of Ryangkeogsanwhatang on the Adipocyte induced by Gold thioglucose in the Rat", The Journal of Korean Oriental Medical Society, 17(2) (1996)
- 고남경, "부분비만 일본인을 대상으로 한 한방 비만치료 증례보고 3예", 한방비만학회지, 15(1), pp.51~54 (2015), 한방비만학회
- Kim Hye-Ju, "Effect of Taeeumjoweetang on the Body Composition", Serum Lipid Level and Antioxidant Enzyme Activity of Obese Female Colleage Students, 23(3), pp.391~401 (2011)
- Lee Jae Ma, "東醫壽世保元", The 2nd Chapter. 四端論, 2~10 (1894)
- Lee Jae Ma, "東醫壽世保元", The 9th Chapter 脾受寒表寒病論, 9~22 (1894)
- 박은아, "少陽人 亡陰證 환자의 治驗 2례", 사상체질의학회지, 18(3), pp.210~215 (2006), 사상체질의학회
- Park In Sun, "The Inhibitory effects of So-yangin Bimanbang on the Obese-Mice induced by High-Fat Diet", Master, pp.2010
- Sung hwan Moon, "The effects of proliferation and differentiation on adipocyte 3T3-L1 by herbs and herbal prescriptions of Soeumin and Soyangin", Journal of KyungHee Oriental Medicine College, 21(1) (1998)
- Hong-won Sik, "Hwnag-jenaegyeong", pp.61;94;219;220;275~ (1991), Oriental Medicine Research Publication
- Kim Sung-Hoon, "The Discussion of the Concept, Diagnosis, Treatment of Obesity in Korean Medicine References", Journal of Haehwa Medicine, 7(1), pp.533~541 (1998)
- 이희영, "輕身降脂丸18의 분자생물학적인 비만조절 기전에 관한 연구", 대한본초학회지, 26(1), pp.65~74 (2011), 대한본초학회
- Jang YJ, "Effect of Taeyeumjoweetang and electroacupuncture combined-therapy on obesity", J Korean Orien Assoc Stud Obes, 1(1), pp.77~83 (2001)
- Hwang DS, "Effect of electro-acupuncture on regional fat by measuring thigh circumstance", J Korean Orient Assoc Stud Obes, 5(1), pp.1~8 (2005)
- Choi HS, "Compative prospective short-term study on effect of electro-acupuncture on thigh circumference", J Korean Orient Assoc Stud Obes, 3(1), pp.61~67 (2003)
- Kim Kyung-yo, "The Effect of Taesoeum-in, Soyang-in Prescriptions in a Gold thioglucose induced obesity mouse model", Journal Of Sasang Constitutional Medicine, 3(1), pp.57~61 (1989)
- Lee GJ, "Effects of Taeyeumjoweetang on obesity of mouse and induced adipocyte 3T3-L1", J Sasang Med, 8(2), pp.219~218 (1996)
- 권오원, "猪苓車前子湯의 基源, 變遷過程 및 構成原理", 사상체질의학회지, 28(2), pp.103~109 (2016), 사상체질의학회[DOI]
- 김중화, "사상체질별 약물의 lipase 저해활성을 통한 항비만효능에 관한 실험적 연구", 동의생리병리학회지, 19(3), pp.710~715 (2005), 한의병리학회
- Bacon BR, "Nonalcoholic steatohepatitis: an expanded clinical entity", Gastroenterology, 107, pp.1103~ (1994)[DOI]
- Kathleen DP, "Mosby's Manual of Diagnostic and Laboratory Tests" (2002), Mosby
- Stein O, "Atheroprotective mechanisms of HDL", Atherosclerosis, 144, pp.285~301 (1999)[DOI]
- Kitamura A, "High-density lipoprotein cholesterol and premature coronary heart disease in urban Japanese men", Circulation, 89, pp.2533~2539 (1994)[DOI]
- Iuliano L, "Radiolabeled native low-density lipoprotein injected into patients with carotid stenosis accumulates in macrophages of atherosclerotic plaque : effect of vitamin E supplementation", Circulation, 101, pp.1249~ (2000)[DOI]
- Anderson TJ, "The effect of cholesterol-lowering and antioxidant therapy on endothelium-dependent coronary vasomotion", N Engl J Med, 332, pp.488~ (1995)[DOI]
- 김재수, "YGF251 투여에 의한 인체내 혈중의 IGF-1 분비량 변화 효과", KSBB Journal, 17(4), pp.403~408 (2002), 한국생물공학회
- Meier U, "Endocrine regulation of energy metabolism: review of pathobiochemical and clinical chemical aspects of leptin, ghrelin, adiponectin, and resistin", Clin Chem, 50, pp.1511~ (2004)[DOI]
- Yamauchi T, "The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity", Nat Med, 7, pp.941~ (2001)[DOI]
- Brun, "Adipocyte differentiation: a transcriptional regulatory cascade", Curr. Opin. Cell. Biol., 8, pp.826~832 (1996)[DOI]
- Cao Z, "Regulated expression of three C", EBP isoforms during adipose conversion of 3T3-L1 cells, 1991, pp.5(9), 1538~1552
- Vidal-Puig A, "UCP3: an uncoupling protein homologue expressed preferentially and abundantly in skeletal muscle and brown adipose tissue", Biochem Biophys Res Commun, 235(1), pp.79~82 (1997)[DOI]
- Ribot J, "Weight loss reduces expression of SREBP1c", ADD1 and PPARgamma2 in adipose tissue of obese women, 2001, pp.441(4), 498~505
