小陽人 忍冬藤地骨皮湯이 中消合倂症에 미치는 影響
Effecs of Soyangin Indongdeungjikolpitang on the Complication of Diabetes
0
조회
0
다운로드
0
인용
0
참고문헌
초록
The purpose of this research was to investigate the effects of Indongdeungjikolpitang water extract(IJTE) on the complication of diabetes. IJTE did not affect the level of blood glucose in alloxan- or streptozotocin-induced hyperglycemic mice, but inhibited the motility of gastrointestine. IJTE inhibited the writhing syndrome induced by acetic acid, the permeability of evans blue into peritoneal cavity induced by acetic acid, the paw edema induced by histamine, and the formation of cotton pellet granuloma. IJTE increased the cell viability of thymocytes and splenocytes. IJTE decreased the release of γ-interferone(γ-IFN) and interleukin-2(IL-2), but did not affect the release of interleukin-4(IL-4) from murine thymocytes. IJTE increased the release of IL-4 and decreased the release of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β), but did not affect of γ-IFN and IL-2 from murine splenocytes. IJTE decreased the release of TNF-α and IL-1β from murine peritoneal macrophages. IJTE decreased the production of niric oxide(NO) from murine peritioneal macrophages and increased the phagocytic activity of murine peritoneal macrophages. These results suggest that IJTE has an anti-inflammatory action via the inhibition of TNF-α, IL-1β and NO production from immune cells. 少陽人 忍冬藤地骨皮湯이 中消 合倂症에 미치는 影響을 살펴보고자 하였고 結果는 다음과 같다. 1. 腸管運動을 抑制하였다. 2. Acetic acid에 의한 writhing syndrome을 抑制하였다. 3. Evans blue에 의한 毛細血管透過性을 抑制하였다. 4. Histamine에 의한 急性足浮腫을 抑制하였다. 5. Cotton pellet에 의한 肉芽腫 形成을 抑制하였다. 6. 胸線細胞 및 脾臟細胞의 細胞生存率을 增加시켰다. 7. 胸線細胞로부터 γ-IFN 및 IL-2의 分泌를 抑制하였다. 8. 脾臟細胞로부터 IL-4의 分泌는 增加시켰으나, TNF-α 및 IL-1β의 分泌는 抑制하였다. 9. 腹腔 大食細胞로부터 TNF-α, IL-1β 및 nitric oxide의 分泌를 抑制하였다. 10. 腹腔 大食細胞의 lucigenin chemiluminescence 및 FITC-conjugated E. cole engulfment를 促進하였다. 이상의 實驗 結果 IJTE는 腸管運動을 抑制하였으며, 鎭痛 및 抗炎作用을 나타냈고, 이들 작용은 免疫細胞로부터 TNF-α, IL-1β 및 nitric oxide의 生成을 抑制하기 때문인 것으로 추정된다. 따라서 이러한 機轉에 의하여 忍冬藤地骨皮湯은 中消 合倂症의 하나인 炎症을 抑制하는데 有效한 것으로 思料된다.
Abstract
The purpose of this research was to investigate the effects of Indongdeungjikolpitang water extract(IJTE) on the complication of diabetes. IJTE did not affect the level of blood glucose in alloxan- or streptozotocin-induced hyperglycemic mice, but inhibited the motility of gastrointestine. IJTE inhibited the writhing syndrome induced by acetic acid, the permeability of evans blue into peritoneal cavity induced by acetic acid, the paw edema induced by histamine, and the formation of cotton pellet granuloma. IJTE increased the cell viability of thymocytes and splenocytes. IJTE decreased the release of γ-interferone(γ-IFN) and interleukin-2(IL-2), but did not affect the release of interleukin-4(IL-4) from murine thymocytes. IJTE increased the release of IL-4 and decreased the release of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β), but did not affect of γ-IFN and IL-2 from murine splenocytes. IJTE decreased the release of TNF-α and IL-1β from murine peritoneal macrophages. IJTE decreased the production of niric oxide(NO) from murine peritioneal macrophages and increased the phagocytic activity of murine peritoneal macrophages. These results suggest that IJTE has an anti-inflammatory action via the inhibition of TNF-α, IL-1β and NO production from immune cells.