Localization patterns of LYVE1 and CD31 in mice tongue development
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Lymphatic vessel endothelial hyaluronan receptor-1 (LYVE1) is a cell surface receptor on lymphatic endothelial cells which is the earliest known marker of lymphatic endothelial commitment. Platelet endothelial cell adhesion molecule also known as cluster of differentiation 31 (CD31) is a well-known marker for blood vessel formation. In this study, we examined the localization patterns of LYVE1 and CD31 in the developing tongue at E14, E16, E17 and PN0, PN5, PN10, PN15 and PN20 to understand the detailed developmental processes of lymphangiogenesis along with vasculogenesis by immunostaining examinations. Our observations revealed that there were specific patterns of proteins localizations through developmental stages. At E14, LYVE1 was localized mainly in the ventral region along the median fibrous septum and laterally in between the genioglossus and hyoglossus muscle forming regions. The localization of CD31 was more ubiquitous in the developing tongue at E14. The localization patterns of LYVE1 and CD31 showed more specified in the dorsal and ventral region at E16. In addition, branching formation of the lymphatic and blood vessels were more evident. At E17, more distinct localization patterns of LYVE1 and CD31 were observed in the intrinsic tongue muscles forming region. At later stages, the LYVE-1 and CD31 were mostly localized in the interface between the muscle fibers. Our results suggest that there would be relationships between lymphangiogenesis and vasculogenesis in developing mouse tongue. These normal developmental processes in lymphangiogenesis and vasculogenesis would be an excellent foundation for further studies in pathological conditions and tongue development malformations.
Abstract
Lymphatic vessel endothelial hyaluronan receptor-1 (LYVE1) is a cell surface receptor on lymphatic endothelial cells which is the earliest known marker of lymphatic endothelial commitment. Platelet endothelial cell adhesion molecule also known as cluster of differentiation 31 (CD31) is a well-known marker for blood vessel formation. In this study, we examined the localization patterns of LYVE1 and CD31 in the developing tongue at E14, E16, E17 and PN0, PN5, PN10, PN15 and PN20 to understand the detailed developmental processes of lymphangiogenesis along with vasculogenesis by immunostaining examinations. Our observations revealed that there were specific patterns of proteins localizations through developmental stages. At E14, LYVE1 was localized mainly in the ventral region along the median fibrous septum and laterally in between the genioglossus and hyoglossus muscle forming regions. The localization of CD31 was more ubiquitous in the developing tongue at E14. The localization patterns of LYVE1 and CD31 showed more specified in the dorsal and ventral region at E16. In addition, branching formation of the lymphatic and blood vessels were more evident. At E17, more distinct localization patterns of LYVE1 and CD31 were observed in the intrinsic tongue muscles forming region. At later stages, the LYVE-1 and CD31 were mostly localized in the interface between the muscle fibers. Our results suggest that there would be relationships between lymphangiogenesis and vasculogenesis in developing mouse tongue. These normal developmental processes in lymphangiogenesis and vasculogenesis would be an excellent foundation for further studies in pathological conditions and tongue development malformations.
참고문헌 (31)
- Noden DM, "The differentiation and morphogenesis of craniofacial muscles", Dev Dyn Off Publ Am Assoc Anat, 235, pp.1194~1218 (2006)
- Shuler CF, "Molecular regulation of tongue and craniofacial muscle differentiation", Crit Rev Oral Biol Med, 12(1), pp.3~17 (2001)
- Parada C, "Molecular and cellular regulatory mechanisms of tongue myogenesis", J Dent Res, 91(6), pp.528~535 (2012)
- Paulson RB, "Scanning electron microscope study of tongue development in the CD-1 mouse fetus", J Craniofac Genet Dev Biol, 5, pp.59~73 (1985)
- Nagata J, "Progress of cell proliferation in striated muscle tissues during development of the mouse tongue", J Dent Res, 83, pp.926~929 (2004)
- Nie X, "Apoptosis, proliferation and gene expression patterns in mouse developing tongue", Anat Embryol, 210, pp.125~132 (2005)
- Buckingham M, "The formation of skeletal muscle: from somite to limb", J Anat, 202, pp.59~68 (2003)
- Yamane A, "Expression of myogenic regulatory factors during the development of mouse tongue striated muscle", Arch. Oral Biol, 45, pp.71~78 (2000)
- Zaidi FN, "Tongue anatomy and physiology, the scientific basis for a novel targeted neurostimulation system designed for the treatment of obstructive sleep Apnea", Neuromodulation, 16, pp.376~386 (2013)
- Moore KL, "The developing human" (2008), Saunders
- Naumova EA, "The oral mucosal surface and blood vessels", Head Face Med, 9, pp.8~ (2013)
- Ryan TJ, "Lymphatics of the skin", Int J Dermatol, 25, pp.411~419 (1986)
- Leak LV, "Electron microscopic observations on lymphatic capillaries and the structural components of the connective tissue–lymph interface", Microvasc Res, 2, pp.361~391 (1970)
- Schmelz M, "Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells. II. Different types of lymphatic vessels", Differentiation, 57, pp.97~117 (1994)
- Gerli R, "Specific adhesion molecules bind anchoring filaments and endothelial cells in human skin initial lymphatics", Lymphology, 33, pp.148~157 (2000)
- Solito R, "An immunological correlation between the anchoring filaments of initial lymph vessels and the neighboring elastic fibers: a unified morphofunctional concept", Lymphology, 30, pp.194~202 (1997)
- Sacchi G, "The structure of superficial lymphatics in the human thigh: precollectors", Anat Rec, 247, pp.53~62 (1997)
- Yang Y, "Development of the mammalian lymphatic vasculature", J Clin Invest, 124(3), pp.888~897 (2014)
- Srinivasan RS, "Lineage tracing demonstrates the venous origin of the mammalian lymphatic vasculature", Genes Dev, 21(19), pp.2422~2432 (2007)
- Yaniv K, "Live imaging of lymphatic development in the zebrafish", Nat Med, 12(6), pp.711~716 (2006)
- Yang Y, "Lymphatic endothelial progenitors bud from the cardinal vein and intersomitic vessels in mammalian embryos", Blood, 120(11), pp.2340~2348 (2012)
- Hägerling R, "A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy", EMBOJ, 32(5), pp.629~644 (2013)
- Wigle JT, "Prox1 function is required for the development of the murine lymphatic system", Cell, 98(6), pp.769~778 (1999)
- Oliver G, "Lymphatic vasculature development", Nat Rev Immunol, 4(1), pp.35~45 (2004)
- Hamada K, "VEGF-C signaling pathways through VEGFR-2 and VEGFR-3 in vasculoangiogenesis and hematopoiesis", Blood, 96, pp.3793~3800 (2000)
- Veikkola T, "Signalling via vascular endothelial growth factor receptor-3 is sufficient for lymphangiogenesis in transgenic mice", EMBOJ, 20, pp.1223~1231 (2001)
- Sebzda E, "Syk and Slp-76 Mutant Mice Reveal a Cell-Autonomous Hematopoietic Cell Contribution to Vascular Development", Dev Cell, 11, pp.349~361 (2006)
- Mäkinen T, "Isolated lymphatic endothelial cells transduce growth, survival and migratory signals via the VEGF-C", D receptor VEGFR-3, 2001, pp.20, 4762~4773
- Jackson DG, "The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1", Trends Cardiovasc. Med, 13, pp.1~7 (2003)
- Kaipainen A, "Expression of the fms-like tyrosine kinase FLT4 gene becomes restricted to lymphatic endothelium during development", Proc Natl Acad Sci USA, 92, pp.3566~3570 (1995)
- Wigle JT, "Prox1 function is required for the development of the murine lymphatic system", Cell, 98, pp.769~778 (1999)