본문 바로가기
KERD
논문KCI 등재

삼차신경입쪽핵에서 치수유래 들신경종말의 면역조직화학적 분석

Postembedding Immunocytochemical Analysis of Tooth Pulp Afferent Terminals in the Trigeminal Nucleus Oralis

한혜민|Jae Youn Son|Tae Heon Kim|Yong-Chul Bae
대한구강해부학회 (The Korean Academy Of Oral Anatomy)|2018.12|대한구강해부학회지 (The Korean Journal of Oral Anatomy)|제39권 제1호|pp. 9-17|DOI 10.35607/kjoa.39.1.201812.002

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

GABApulpal afferent시냅스 연결성시냅스이전억제synaptic connectivitypresynaptic inhibition

초록

삼차신경입쪽핵(trigeminal nucleus oralis)은 치수유래 들신경섬유로부터 통각정보를 전달받아 하악반사에 관여하는 것으로 알려져 있다. 하지만 치수에서 유래하는 통각정보가 삼차신경입쪽핵에서 전달되고처리되는데 관여하는 신경전달물질에 대해서는 잘 알려져 있지 않다. 이를 밝히기 위해 본 연구에서는 신경추적자(WGA-HRP)를 이용하여 삼차신경입쪽핵으로 투사하는 치수유래 들신경섬유의 종말을 표식한 후전자현미경적 면역조직화학기법을 시행하여 다음과 같은 결과를 얻었다. 삼차신경입쪽핵에서 치수유래 표식종말은 직경 45-55 ㎚의 둥근 모양의 소포를 함유하고 있었으며 일부에서 직경 90-120 ㎚의 큰 치밀소포(dense cored vesicles)가 관찰되었다. 표식종말은 대부분이 중간부분(middle dendrite) 혹은 먼쪽(distal dendrite) 가지돌기와 비대칭 연접을 이루었으며 세포체(soma)나 몸쪽가지돌기(proximal dendrite)와 연접하는 경우는 관찰되지 않았다. 표식종말은 여러 형태의 소포를 함유하는 종말(p-ending)과 대칭연접을 이루었는데 이들 종말내의 연접부위에서 소포들이 밀집되어 있는 것으로보아 이들로부터 연접신호를 전달받는 것으로 나타났다. 금입자면역조직화학기법에 의한 면역반응 결과 일차들신경종말 및 이와 연접하는 p-ending은 각각 글루탐산염 및 GABA에 양성반응을 나타내었다. 이상의 결과로 미루어보아 삼차신경입쪽핵에 투사하는 치수유래 신경섬유의 말단은 글루탐산염을 신경전달물질로 사용하며 이는 GABA를 신경전달물질로 사용하는 p-ending에 의하여 조절을 받는 것으로 생각된다.

Abstract

Trigeminal nucleus oralis (Vo) receiving nociceptive information from the dental pulp is involved in reflexive jaw movement. However, little information is available regarding the neurotransmitters which are involved in the transmission and processing of pulpal nociceptive information in the Vo. To address this issue, we labeled afferents of rat upper molar pulps using retrograde wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) tracing, performed postembedding immunocytochemistry with glutamate and GABA antisera, and ultrastructural analysis. The labeled pulpal afferent terminals in the Vo contained many spherical synaptic vesicles (45-55 nm in diameter). They frequently made asymmetrical synaptic contacts with the middle or distal parts of dendrites rather than the soma or proximal part of dendrites. They received synapse from pleomorphic vesicles containing presynaptic endings. Gold particles labeling the glutamate were densely distributed in the afferent terminals of the tooth pulp but were sparse in the presynaptic endings. On the other hand, gold particles labeling GABA were densely distributed in the presynaptic endings, but sparse in the afferent terminals of the tooth pulp. These findings indicate that tooth pulp afferent terminals use glutamate as a neurotransmitter in the Vo and they are controlled by presynaptic endings that use GABA as a neurotransmitter.

참고문헌 (42)

  1. Shigenaga Y, "Topographic representation of lower and upper teeth within the trigeminal sensory nuclei of adult cat as demonstrated by the transganglionic transport of horseradish peroxidase", J Comp Neurol, 251, pp.299~316 (1986)[DOI]
  2. Shigenaga Y, "Morphology of single mesencephalic trigeminal neurons innervating masseter muscle of the cat", Brain Res, 445, pp.392~399 (1988)[DOI]
  3. Shigenaga Y, "Morphological and functional properties of trigeminal nucleus oralis neurons projecting to the trigeminal motor nucleus of the cat", Brain Res, 416, pp.143~149 (1988)[DOI]
  4. Yoshida A, "Two major types of premotoneurons in the feline trigeminal nucleus oralis neurons as demonstrated by intracellular stianing with HRP", J Comp Neurol, 347, pp.495~514 (1994)[DOI]
  5. Sugimoto T, "Induction of immediate-early genes c-fos and zif268in the subnucleus oralis by noxious tooth pulp stimulation", Brain Res, 794, pp.353~358 (1998)[DOI]
  6. Sugimoto T, "c-fos induction in the subnucleus oralis following trigeminal nerve stimulation", Brain Res, 783, pp.158~162 (1998)[DOI]
  7. Oakden EL, "Fos expression in the ferret trigeminal nuclear complex following tooth pulp stimulation", Neurosci, 84, pp.1197~1208 (1998)[DOI]
  8. Broman J, "Enrichment of glutamate-like immunoreactivityin primary afferent terminals throughout the spinal cord dorsal horn", Eur J Neurosci, 5, pp.1050~1061 (1993)[DOI]
  9. Valtschanoff JG, "Amino acid immucocytochemistry of primary afferent terminals in the rat dorsal horn", J Comp Neurol, 346, pp.237~252 (1994)[DOI]
  10. Örnung G, "Ia boutons to CCN neurons and motoneurons are enriched with glutamate-like immunoreactivity", Neuroreport, 6, pp.1975~1980 (1995)[DOI]
  11. Bae YC, "Identification of signal substances in synapses made between primary afferents and their associated axon terminals in the rat trigeminal sensory nuclei", J Comp Neurol, 418, pp.299~309 (2000)
  12. Clements JR, "Rat tooth pulp projections to spinal trigeminal subnucleus caudalis are glutamate-like immunoreactive", J Comp Neurol, 309, pp.281~288 (1991)[DOI]
  13. Mesulam MM, "Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents", J Histochem Cytochem, 26, pp.106~117 (1978)[DOI]
  14. Weinberg RJ, "A tetramethylbenzidine", tungstate reaction for horseradish peroxidase histochemistry, 1991, pp.39, 1143~1148
  15. Storm-Mathisen J, "First visualization of glutamate and GABA in neurons by immunocytochemistry", Nature, 301, pp.517~520 (1983)[DOI]
  16. Kolston J, "An atlas of glycine- and GABA-like immunoreactivity and colocalization in the cochlear nuclear complex of the guinea pig", Ant Embryol, 186, pp.443~465 (1992)[DOI]
  17. Ottersen OP, "Evaluation of the immunocytochemical method of amino acids", Med Biol, 64, pp.147~158 (1986)
  18. Kristensen K, "Axonal uptake and retrograde transport of exogenous proteins in the hypoglossal nerve", Brain Res, 32, pp.399~406 (1971)[DOI]
  19. La Vail JH, "Retrograde axonal transport in the central nervous system", Science, 176, pp.1416~1417 (1972)[DOI]
  20. Ganser AL, "Ganglioside localization on myelinated nerve fibres by cholera toxin binding", J Neurocytol, 12, pp.921~938 (1983)[DOI]
  21. Trojanowski JQ, "Time of arrival of wheat germ agglutinin-HRP conjugates in superior colliculus after intraocular injections in the rat", Brain Res, 267, pp.365~370 (1983)[DOI]
  22. Scott SA, "Lectin binding identifies a subpopulation of neurons in chick dorsal root ganglia", J Neurosci, 10, pp.336~345 (1990)[DOI]
  23. Robertson B, "Transganglionic transport of wheat germ agglutinin-HRP and choleragenoid-HRP in rat trigeminal primary sensory neurons", Brain Res, 348, pp.44~51 (1985)[DOI]
  24. Robertson B, "A comparison between wheat germ agglutinin-and choleragenoid-horseradish peroxidase as anterogradely transported markers in central branches of primary sensory neurones in the rat with some observations in the cat", Neurosci, 14, pp.895~905 (1985)[DOI]
  25. Weinberg RJ, "Extracellular labeling of unmyelinated dorsal root terminals after WGA-HRP injections in spinal ganglia", Brain Res, 523, pp.351~355 (1990)[DOI]
  26. Curtis DR, "Chemical excitation of spinal neurons", Nature, 183, pp.611~612 (1959)[DOI]
  27. Willcockson WS, "Effects of iontophoretically released amino acids and amines on primate spinothalamic tract cells", J Neurosci, 4, pp.732~740 (1984)[DOI]
  28. Todd AJ, "The localization of classical transmitters and neuropeptides within neurons in laminae I-III of the mammalian spinal dorsal horn", Prog Neurobiol, 41, pp.609~645 (1993)[DOI]
  29. Clements JR, "An electron microscopic description of glutamate-like immunoreactive axon terminals in the rat principal sensory and spinal trigeminal nuclei", J Comp Neurol, 309, pp.271~280 (1991)[DOI]
  30. Iliakis B, "Electron microscopy of immunoreactivity patterns for glutamate and gamma-aminobutyric acid in synaptic glomeruli of the feline spinal trigeminal nucleus (Subnucleus Caudalis)", J Comp Neurol, 366, pp.465~477 (1996)
  31. 서유헌, "신경전달물질", pp.267~272 (1992), 민음사
  32. Gray EG, "A morphological basis for pre-synaptic inhibition", Nature, 193, pp.82~83 (1962)[DOI]
  33. Barber RP, "GABAergic terminals are presynaptic to primary afferent terminals in the substantia gelatinosa of the rat spinal cord", Brain Res, 141, pp.35~55 (1978)[DOI]
  34. Todd AJ, "GABA-immunoreactive neurons in the dorsal horn of the rat spinal cord", Neurosci, 31, pp.799~806 (1989)[DOI]
  35. Maxwell DJ, "Direct observations of synapses between GABA-immunoreactive boutons and muscle afferent terminals in lamina Ⅵ of the cat's spinal cord", Brain Res, 530, pp.215~222 (1990)[DOI]
  36. Maxwell DJ, "Synaptic connections of GABA-containing boutons in the lateral cervical nucleus of the cat: an ultrastructural study employing pre- and post-embedding immunocytochemical methods", Neurosci, 33, pp.169~184 (1989)[DOI]
  37. Basbaum AI, "Immunoreactive glutamic acid decarboxylase in the trigeminal nucleus caudalis of the cat: a light- and electron-microscopic analysis", Somatosens Res, 4, pp.77~94 (1986)[DOI]
  38. Almond JR, "Post-embedding immunogold labeling of gamma-aminobutyric acid in lamina II of the spinal trigeminal subnucleus pars oralis. I. A quantitative study", Synapse, 24, pp.39~47 (1996)
  39. Dumba JS, "Electron microscopic analysis of gamma-aminobutyric acid and glycine colocalization in rat trigeminal subnucleus caudalis", Brain Res, 806, pp.16~25 (1998)[DOI]
  40. Bae YC, "Electron microscopic observation of synaptic connections of jaw-muscle spindle and periodontal afferent terminals in the trigeminal motor and supratrigeminal nuclei in the cat", J Comp Neurol, 374, pp.421~435 (1996)
  41. Yoshida A, "Organization of the descending projections from the parabrachial nucleus to the trigeminal sensory nuclear complex and spinal dorsal horn in the rat", J Comp Neurol, 383, pp.94~111 (1997)
  42. Zhang J, "Light and electron microscopic observations of a direct projection from mesencephalic trigeminal nucleus neurons to hypoglossal motoneurons in the rat", Brain Res, 917, pp.67~80 (2001)[DOI]

관련 논문AI추천

흰쥐의 삼차신경주감각핵에서 치수유래 일차들신경종말 및 이와 연접하는 연접이전신경종말의 연접소포 형태 비교곽우경, 김윤숙, 김지만, 박매자, 배용철, 백상규, 조이슬한국현미경학회 · Applied Microscopy2008 · 제38권 제4호Quantitative Ultrastructural Analysis of Endings Presynaptic to the Tooth Pulp Afferent Terminals in the Trigeminal Oral Nucleus김태헌, 박숙경, 배용철, 이석기, 이천희대한구강생물학회 · International Journal of Oral Biology2016 · 제41권 제3호흰쥐 삼차신경 감각핵에 존재하는 이온통로의 분포에관한 면역조직화학적 연구박호영, 최기운, 최호영대한치과보존학회 · Restorative Dentistry and Endodontics2002 · 제27권 제3호고양이 삼차신경꼬리핵에서 저역치기계자극수용기 유래 들신경종말의 연접이전종말이 함유하는 신경전달물질에 대한 정량적 분석김윤숙, 나연경, 문철주, 배용철, 배진영, 백상규, 복혜정, 조진현한국현미경학회 · Applied Microscopy2012 · 제42권 제1호흰쥐 삼차신경감각핵에서 P2X3와 TRPV1의 공존에 관한 연구김윤숙, 김홍태, 류창현, 문용석, 문제일, 박매자, 배용철, 백상규, 조이슬한국현미경학회 · Applied Microscopy2008 · 제38권 제3호치수유래 구심성 신경섬유의 삼차신경 감각핵군에서의 연접특성김영진, 김현정, 남순현, 배용철, 이은희, 최민기, 홍수형대한소아치과학회 · 대한소아치과학회지2001 · 제28권 제2호

이 간행물의 다른 논문 (7건)

Expression patterns of Zfhx1a and Zfhx1b during mouse craniofacial development이종민, 정한성, 신정오, 복진웅pp. 1-8Morphological analysis of the temporomandibular joint in patients with malocclusion using three-dimensional modelsJeong-Hyun Lee, 전은영, 박종태, 안현나, Sa-Beom Parkpp. 19-26치아 탈구시 이용할 수 있는 용액에서의 MC3T3-E1 세포의생존력 비교연구Heesu Lee, Yu Ri Kimpp. 27-36Altered VEGF Regulation in Ovariectomized Rat Alveolar Bone Sockets by BisphosphonateGeum-Dong Han, Sun-Hun Kim, Dong-Wook Yangpp. 37-51한국인 삼차신경통 환자에 대한 역학조사곽현호, Min-Kyu Parkpp. 53-59
원문 보기