구강편평상피세포암종에서 Elaiophylin이 유도하는 세포자멸사와 상피간엽이행 억제 효과
Elaiophylin Induces Apoptosis and Inhibits the Epithelial Mesenchymal Transition in OSCC Cell Lines
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초록
Elaiophylin은 Streptomyces melanosporus 균에서 분리한 화합물로 항균, 항바이러스, 면역억제 등의 약리효과를 나타내며 발표된 논문에 따르면 난소암, 폐암, 흑색종 등 다양한 종양세포에서 항암활성 효과를나타내는 것으로 입증된 바 있다. 그러나 구강편평상피세포암종에서의 elaiophylin의 약리효과에 대해서는 아직 밝혀진 바가 없으므로 본 연구에서는 구강편평상피세포암종 세포주인 Ca9-22와 CAL-27세포에elaiophylin을 처리하여 세포자멸사와 상피간엽이행 매커니즘에 미치는 영향을 확인함으로써 elaiophylin이구강암의 치료제로서의 효용가치가 있는지 확인하기 위한 실험을 시행하였다. 그 결과 elaiophylin은 Ca9- 22와 CAL-27 세포에서 내인성 경로를 통한 세포자멸사를 유도하였고 암세포의 상피간엽이행에 관여하는단백질의 발현을 조절하여 항암 활성 효과를 나타내었음을 입증하였다.
Abstract
Elaiophylin, isolated from Streptomyces melanosporus, exhibits pharmacological effects, such as antimicrobial, antiviral, and immunosuppressive activities. According to published studies, elaiophylin has been proven to demonstrate anticancer activity in various tumor cells including ovarian cancer, lung cancer, and melanoma. However, the pharmacological effects of elaiophylin on oral squamous cell carcinoma (OSCC) cells have not been elucidated. Therefore, in this study, we treated the OSCC cell lines Ca9-22 and CAL-27 with elaiophylin to investigate its impact on cell apoptosis and the epithelial-mesenchymal transition (EMT) mechanism. The study aimed to assess the potential of elaiophylin as a therapeutic agent for oral cancer. The results revealed that elaiophylin induced apoptosis through intrinsic pathways in Ca9-22 and CAL-27 cells and demonstrated anticancer activity by regulating the expression of proteins involved in the epithelial-mesenchymal transition. These findings support the potential of elaiophylin as a treatment for oral cancer.
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