Antioxidant and Anti-Stress Activity of Shepherd’s Purse
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In order to determine the antioxidant abilities of different parts of shepherd’s purse (Capsella brusa-pasteris) of Brassicaceae, total polyphenol and flavonoid contents, ABTS and DPPH free radical scavenging activities were measured using three extraction solvents: hot water, 70% ethanol and 100% methanol. The proline content for each solvent extract was also determined to ascertain anti-stress activity along with SOD-like activity. The results showed that 70% ethanol is the best solvent for the extraction of polyphenol from the scape with flowers of shepherd’s purse, whereas 100% methanol is the best solvent for the extraction of flavonoid from the leaf scape with leaves. The lowest contents of polyphenol and flavonoid compounds were obtained from hot water and 100% methanol extracts of the roots, respectively. The highest free radical scavenging activity determined by the ABTS and DPPH assays was observed in 70% ethanol extract of the scape with flowers while the lowest activity was seen in hot water extract of the roots. The highest SOD-like activity was observed in 70% ethanol extract of the scape with flowers among the fractions of samples of shepherd’s purse and the lowest from the 100% methanol extract of the roots. Lastly, the highest proline content was observed from the 100% methanol extract of the scape with flowers and the lowest content from hot water extract of the leaf scape with leaves of shepherd’s purse. The studies showed that all parts of shepherd’s purse have high antioxidant activity and anti-stress activity, Overall, these results support that shepherd’s purse can be used as a natural antioxidant.
Abstract
In order to determine the antioxidant abilities of different parts of shepherd’s purse (Capsella brusa-pasteris) of Brassicaceae, total polyphenol and flavonoid contents, ABTS and DPPH free radical scavenging activities were measured using three extraction solvents: hot water, 70% ethanol and 100% methanol. The proline content for each solvent extract was also determined to ascertain anti-stress activity along with SOD-like activity. The results showed that 70% ethanol is the best solvent for the extraction of polyphenol from the scape with flowers of shepherd’s purse, whereas 100% methanol is the best solvent for the extraction of flavonoid from the leaf scape with leaves. The lowest contents of polyphenol and flavonoid compounds were obtained from hot water and 100% methanol extracts of the roots, respectively. The highest free radical scavenging activity determined by the ABTS and DPPH assays was observed in 70% ethanol extract of the scape with flowers while the lowest activity was seen in hot water extract of the roots. The highest SOD-like activity was observed in 70% ethanol extract of the scape with flowers among the fractions of samples of shepherd’s purse and the lowest from the 100% methanol extract of the roots. Lastly, the highest proline content was observed from the 100% methanol extract of the scape with flowers and the lowest content from hot water extract of the leaf scape with leaves of shepherd’s purse. The studies showed that all parts of shepherd’s purse have high antioxidant activity and anti-stress activity, Overall, these results support that shepherd’s purse can be used as a natural antioxidant.
참고문헌 (30)
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