The Effect of Hypothermia on Cognitive Impairment and Anxiety-like Behavior in a Cardiac Arrest Mouse Model
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Cardiac arrest (CA) is a severe health condition that can lead to significant neurological deficits. While therapeutic hypothermia (TH) has been shown to improve neurological outcomes and survival rates in CA patients, its effect on cognitive impairment is uncertain. In this study, we aimed to investigate the impact of TH on a mouse model of CA-induced cognitive impairment. The model was created by occluding the bilateral common carotid arteries while monitoring the cerebral blood flow with Laser Doppler flowmetry. Nine-week-old male C57BL/6 mice were randomly assigned to one of the three groups: sham, CA, and TH. We performed three distinct behavior tests to evaluate short-term memory, anxiety and exploratory behaviors, and spatial learning and memory function: the Y-maze test, open field test, and the Morris water maze test, respectively. Our findings indicate that the CA group displayed cognitive impairment in all behavior tests compared to the sham group. Although TH tended to improve the spatial motor memory and alleviate anxiety-like behavior in the CA mouse model, it significantly improved the spatial learning and memory function. Overall, these results suggest that TH can enhance cognitive impairment, a typical feature of AD, after CA in mice, although the molecular and biological mechanisms of TH require further investigation.
Abstract
Cardiac arrest (CA) is a severe health condition that can lead to significant neurological deficits. While therapeutic hypothermia (TH) has been shown to improve neurological outcomes and survival rates in CA patients, its effect on cognitive impairment is uncertain. In this study, we aimed to investigate the impact of TH on a mouse model of CA-induced cognitive impairment. The model was created by occluding the bilateral common carotid arteries while monitoring the cerebral blood flow with Laser Doppler flowmetry. Nine-week-old male C57BL/6 mice were randomly assigned to one of the three groups: sham, CA, and TH. We performed three distinct behavior tests to evaluate short-term memory, anxiety and exploratory behaviors, and spatial learning and memory function: the Y-maze test, open field test, and the Morris water maze test, respectively. Our findings indicate that the CA group displayed cognitive impairment in all behavior tests compared to the sham group. Although TH tended to improve the spatial motor memory and alleviate anxiety-like behavior in the CA mouse model, it significantly improved the spatial learning and memory function. Overall, these results suggest that TH can enhance cognitive impairment, a typical feature of AD, after CA in mice, although the molecular and biological mechanisms of TH require further investigation.
참고문헌 (41)
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