Factor Ⅷ 검사에 Heparin이 미치는 영향에 대한 증례 보고

Abstract

Factor Ⅷ assay is a test for diagnosis of hemophilia A, and a way to monitor after factor replacement therapy and risk assessment of premature atherosclerotic vascular disease. The purpose of this study was to suggest the cases in which the factor Ⅷ assay was affected by heparin contamination in factor Ⅷ assay samples drawn from heparin therapy patients. According to the guide for the Factor Ⅷ assay in NCCLS (National Committee for Clinical Laboratory Standards), two types of reference curves are employed, an extended curve and a working curve. ’The reference and patient results should be inspected for outliers, parallelism, and linearity. We perform a factor Ⅷ assay according to the NCCLS guideline 4 point calibration curve and 1: 10, 1:20 diluted samples for patients. However, 4 cases of heparin contamination showed the reverse result of the patient’s sample. There was an exact error in linearity and parallelism between the standard curve and patients curve. The dilution used for patients of heparin contamination are 1: 10 to 1:40 and compare with the calibration curve. We analyzed the heparin concentration on heparin contaminated plasma. If you assay three dilutions of test plasma on the patients, you could detect the error of the result affected heparin.