동충하초 nutans에 의해 생산 된 Protease의 생화학 적 Characterization
Biochemical Characterization of Protease Produced by Cordyceps nutans
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
.
Abstract
The fruiting body of Cordyceps is derived from the pupa or larva of insects infected by the entomopathogenic fungi Cordyceps. The fruiting body has been used as an anti-cancer and anti-inflammatory ingredient in traditional Chinese medicine. The biochemical characteristics of protease isolated from Cordyceps nutans were investigated in this study. The culturing period for production of protease by C. nutans was 10days. The acidity was pH 7.0, and the temperature was 25℃. The carbon and nitrogen sources for the production of the protease were glucose and yeast extract, respectively. The ratio of C/N was 2% glucose and 0.6% yeast extract. 0.06% CuSO4 was used as the inorganic salt. The investigation into the acidity of the protease produced by C. nutans revealed that the optimal pH and temperature were pH 7.0 and 30℃. The stability of the protease was shown as pH 6.0∼9.0 and 30∼50℃. The investigation into the influence of the metal ions on the enzyme activation of C. nutans revealed that it was inhibited in ZnSO4 and activated in FeSO4.
참고문헌 (25)
- Blakesley RW, "A kinetic and structural characterization of adenosine-5′ -triphosphate:ribonucleic acid adenylyl-transferase from Pseudomonas putida", Biochim. Biophys. Acta, 414(2), pp.133~145 (1975)
- Braun V, "Excretion of a protease by Serratia marcescens", Tech Microbiol, 124, pp.55~61 (1980)
- Mass production and useful material development of the entomopathogenic fungi (Cordyceps) growing on the silkworm. Bombyx mori L, "Cho SY", Rural Development Agaricultural, 1~234 (1999)
- Cory JG, "Incorporation of cordycepin(3′-deoxyadenosine) into ribonucleic acid of human tumor cells", Biochim. Biophys. Acta, 103(4), pp.646~653 (1965)
- CHO, HYUN-JEONG, "Inhibitory Effects of Cordycepin (3'-Deoxyadenosine), a Component ofCordyceps militaris, on Human Platelet Aggregation Induced by Thapsigargin", Journal of Microbiology and Biotechnology, 17(7), pp.1134~1138 (2007), 한국미생물·생명공학회
- Choi IY, "The production of artificial fruiting body of Paecilomyces japonica", Kor. J. Mycol, 27(2), pp.87~93 (1999)
- Jae-Sung Kim, "A Fibrinolytic Enzyme from the Medicinal Mushroom Cordyceps militaris", The Journal of Microbiology, 44(6), pp.622~631 (2006), 한국미생물학회
- Lee JK, "Investigation on culture characteristics of mycelial growth by Cordyceps scarabaeicola", Kor. J. Mycol, 28(2), pp.81~87 (2000)
- 이재근, "풍뎅이동충하초(Cordyceps scarabaeicola)의 자실체 형성 조건", 한국균학회지, 30(1), pp.11~17 (2002), 한국균학회
- Lovinger GG, "The effect of cordycepin on cell transformation by RNA tumor viruses", Virology, 55(2), pp.524~526 (1973)
- Luis V. Lopez-Llorca, "Degradation of insect cuticle by Paecilomyces farinosus proteases", Mycological Progress, 1(3), pp.249~256 (2002)
- Richardson LS, "Effect of cordycepin on the replication of type-cRNA tumor viruses", Int. J. Cancer, 15, pp.451~456 (1975)
- Shin KH, "Antioxidant and immunostimulating activities of the fruiting bodies of Paecilomyces japonica, a new type of Cordyceps sp", Health Aging for Functional Longevity, 928, pp.261~273 (2001)
- Smuckler EA, "Inhibition of hepatic deoxyribonucleic acid-dependent ribonucleic acid polymerases by the exotoxin of Bacillus thringiensis in comparison with the effects of amanitin and cordycepin", Biochem. J, 129(1), pp.153~166 (1972)
- 성재모, "번데기동충하초(Cordyceps militaris)의 균사 생장", 한국균학회지, 30(1), pp.1~5 (2002), 한국균학회
- 성재모, "번데기동충하초의 자실체 형성에 관한 연구", 한국균학회지, 30(1), pp.6~10 (2002), 한국균학회
- Shim JO, "The cultural conditions affecting the mycelial growth of Griofola umbellata", Kor. J. Mycol, 25(3), pp.209~218 (1997)
- 김회창, "눈꽃동충하초로부터 혈전분해효소 정제 및 특성 분석", pp.2010
- 김재성, "밀리타리스동충하초와 약용버섯으로부터 혈전용해효소 정제 및 특성 분석", pp.2002
- 남성희, "동충하초 자원의 효율적 이용에 관 한 고찰", 한국잠사학회지, 46(1), pp.18~22 (2004)
- 성재모, "동충하초속균의형태적인 특징과 단백질 pattern에의한 계통분류", 한국균학회지, 23, pp.92~104 (1995)
- 성재모, "동충하초의 생태와 분류" (1999), 한국균학회
- 성재모, "동충하초속균의 분포및 Cordyceps miltaris와 C. nutans의 이용에 관한 연구", 한국균학회지, 21(2), pp.94~105 (1993)
- 이해미, "밀리타리스 동충하초(Cordyceps militaris) 열수추출물의 항암활성 및 면역 조절작용에 미치는 영향", 박사, pp.2004
- 유관희, "화경버섯의 배양조건에 따른 균사생장 및 섬유질분해효소 활성에 관한 연구", 한국균학회지, 31(1), pp.14~21 (2003), 한국균학회