Expression of Microsatellite Instability (MSI) from Colorectal Carcinoma Patients
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The death toll of Colorectal Carcinoma in Korea was 1,826 and 7,721 in the years 1992 and 2011, respectively. This rate of increase was shown to be more than 4.23 times higher than that of any other form of cancer. Therefore, Colorectal Carcinoma requires various diagnostic methods, and Microsatellite Instability (MSI) was applied as a new diagnostic tool. From this study with several microsatellite markers, only marker #13 was detected and observed D13S160 13% (4/30), D13S292 13% (4/30), D13S153 10% (3/30) in order. From the results of amplication with microsatellite marker, D13S292 37% (11/30), D13S153 33% (10/30), D13S160 33% (10/30) in order were shown. The appearance of a genetic mutation, which depends on the loci of Colorectal Carcinoma, was shown amplication from rectal cancer (3.77) which was higher than that of right Colorectal Carcinoma (2.08) (p＜0.018). The genetic mutation with lymph node (4.13) appeared higher than normal (1.93) (p＜0.001). There were no great differences in the genetic mutation dependent on disease, histological classification and increased group of serum CEA. Accordingly, it is suggested that the correct primers, which can evaluate MSI well from colorectal carcinoma, should be chosen and that MSI be considered a good prognosis and quality control tool.
Abstract
The death toll of Colorectal Carcinoma in Korea was 1,826 and 7,721 in the years 1992 and 2011, respectively. This rate of increase was shown to be more than 4.23 times higher than that of any other form of cancer. Therefore, Colorectal Carcinoma requires various diagnostic methods, and Microsatellite Instability (MSI) was applied as a new diagnostic tool. From this study with several microsatellite markers, only marker #13 was detected and observed D13S160 13% (4/30), D13S292 13% (4/30), D13S153 10% (3/30) in order. From the results of amplication with microsatellite marker, D13S292 37% (11/30), D13S153 33% (10/30), D13S160 33% (10/30) in order were shown. The appearance of a genetic mutation, which depends on the loci of Colorectal Carcinoma, was shown amplication from rectal cancer (3.77) which was higher than that of right Colorectal Carcinoma (2.08) (p＜0.018). The genetic mutation with lymph node (4.13) appeared higher than normal (1.93) (p＜0.001). There were no great differences in the genetic mutation dependent on disease, histological classification and increased group of serum CEA. Accordingly, it is suggested that the correct primers, which can evaluate MSI well from colorectal carcinoma, should be chosen and that MSI be considered a good prognosis and quality control tool.
참고문헌 (18)
- Bocker T, "Microsatellite instability analysis: a multicenter study for reliability and quality control", Cancer Res, 57, pp.4739~4743 (1997)
- Campanella NC, "Optimization of a pentaplex panel for MSI analysis without control DNA in a Brazilian population:correlation with ancestry markers", Eur J Hum Genet, 256, pp.1038~1045 (2013)
- Castagnaro A, "Microsatellite analysis of induced sputum DNA in patients with lung cancer in heavy smokers and in healthy subjects", Exp Lung Res, 33, pp.289~301 (2007)
- Gerber HP, "Differential transcriptional regulation of the two vascular endothelial growth factor receptor genes. Flt-1, but not Flk-1", KDR, is up-regulated by hypoxia, 1997, pp.272, 23659~23667
- Hoffmeister M, "Body Mass Index and Microsatellite Instability in Colorectal Cancer: A Population-based Study", Cancer Epidemiol Biomarkers Prev, 22, pp.2303~2311 (2013)
- Je EM, "Frameshift mutations of axon guidance genes ROBO1 and ROBO2 in gastric and colorectal cancers with microsatellite instability", Pathology, 45, pp.645~650 (2013)
- Juon HS, "Predictors of older Korean Americans' participation in colorectal cancer screening", J Cancer Educ, 18, pp.37~42 (2003)
- Macdonald GA, "Microsatellite instability and loss of heterozygosity at DNA mismatch repair gene loci occurs during hepatic carcinogenesis", Hepatology, 28, pp.90~97 (1998)
- Murayama-Hosokawa S, "Genome-wide single-nucleotide polymorphism arrays in endometrial carcinomas associate extensive chromosomal instability with poor prognosis and unveil frequent chromosomal imbalances involved in the PI3-kinase pathway", Oncogene, 29, pp.1897~1908 (2010)
- Paredes-Zaglul A, "Analysis of colorectal cancer by comparative genomic hybridization:evidence for induction of the metastatic phenotype by loss of tumor suppressor genes", Clin Cancer Res, 4, pp.879~886 (1998)
- Ried T, "Comparative genomic hybridization reveals a specific pattern of chromosomal gains and losses during the genesis of colorectal tumors", Genes Chromosomes Cancer, 15, pp.234~245 (1996)
- Schwarzenbach H, "Microsatellite analysis of allelic imbalance in tumour and blood from patients with prostate cancer", BJU Int, 102, pp.253~258 (2008)
- Shin Al, "Colorectal cancer mortality in Hong Kong of China, Japan, South Korea, and Singapore", World J Gastroenterol, 19, pp.979~983 (2013)
- Sunde L, "Familial colorectal cancer, can it be identified by microsatellite instability and chromosomal instability? - A case-control study", Cancer Biomark, 5, pp.197~205 (2009)
- Trautmann K, "Chromosomal instability in microsatellite-unstable and stable colon cancer", Clin Cancer Res, 21, pp.6379~6385 (2006)
- Xie D, "The significance and characteristics of chromosomal abnormalities in patients with microsatellite and chromosome stable colorectal carcinoma-Article in Chinese", Zhonghua Yi Xue Za Zhi, 87, pp.11~15 (2007)
- Yamamoto Hl, "Gastric cancers of the microsatellite mutator phenotype display characteristic genetic and clinical features", Gastroenterology, 116, pp.1348~1357 (1999)
- Ziadi S, "Clinicopathologic characteristics of colorectal cancer with microsatellite instability", Pathol Res Pract, 210, pp.98~104 (2014)