다발성골수종 환자의 파라핀포매조직에서 MicroRNA 발현
Expression of Micro RNA in Paraffin Embedded Tissue of Multiple Myeloma
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Research of thyroid cancer, liver cancer, and lung cancer has been reported in Korea. However microRNA research of multiple myeloma has never been reported. Hence we intended to confirm whether microRNA can be utilized as a diagnostic marker to patients of multiple myeloma. We also intended to evaluate whether microRNA can be detected in paraffin-embedded tissue (FFPE). This research was conducted targeting 8 samples from patients of multiple myeloma who do not have any other diseases, and 2 control samples. From January 2010 to July 2012, we selected miR-15a, miR-16, miR-21, miR-181a and miR-221 as microRNA target genes. It was decided that for a sample to be significant, the results should show values more than 1.5 or less than -1.5. Our findings of fold change were highly significant in miR-15a with a value of 37.5% (3/8). From these studies, we learned that miR-15a is useful with westerners. miR-221, on the other hand, shows conflicts with westerners, so more research will be needed in this area. In addition, it was confirmed that microRNA can be detected in paraffin embedded tissue (FFPE).
Abstract
Research of thyroid cancer, liver cancer, and lung cancer has been reported in Korea. However microRNA research of multiple myeloma has never been reported. Hence we intended to confirm whether microRNA can be utilized as a diagnostic marker to patients of multiple myeloma. We also intended to evaluate whether microRNA can be detected in paraffin-embedded tissue (FFPE). This research was conducted targeting 8 samples from patients of multiple myeloma who do not have any other diseases, and 2 control samples. From January 2010 to July 2012, we selected miR-15a, miR-16, miR-21, miR-181a and miR-221 as microRNA target genes. It was decided that for a sample to be significant, the results should show values more than 1.5 or less than -1.5. Our findings of fold change were highly significant in miR-15a with a value of 37.5% (3/8). From these studies, we learned that miR-15a is useful with westerners. miR-221, on the other hand, shows conflicts with westerners, so more research will be needed in this area. In addition, it was confirmed that microRNA can be detected in paraffin embedded tissue (FFPE).
참고문헌 (25)
- Bang SM, "Identification of 13q deletion, trisomy 1q and IgH rearrangement as the most frequent chromosomal changes found in Korean patients with multiple myeloma", Cancer Genetics and Cytogenetics, 168, pp.124~132 (2006)
- Chang HJ, "The relationship between the progression of colorectal cancer and microRNA polymorphism", Journal of Clinical Oncology, 7, pp.50~57 (2011)
- Corthals SL, "Micro-RNA-15a and micro-RNA-16expression and chromosome 13 deletions in multiple myeloma", Leukemia Resarch, 34, pp.677~681 (2010)
- Croce CM, "Causes and consequences of microRNA dysregulation in cancer", Nature Reviews Genetics, 10, pp.704~714 (2009)
- Farazi TA, "MicroRNAs in human cancer", Journal of Pathology, 223, pp.102~115 (2011)
- 한경희, "Effects of Promoter Methylation on the Expression Levels of Plakoglobin Gene in Both the ARO Thyroid Cancer Cell Line and Cancer Tissues", 대한임상검사과학회지, 41(4), pp.180~188 (2009), 대한임상검사과학회
- Iorio MV, "MicroRNAs in cancer: small molecules with a huge impact", American Society of Clinical Oncology, 27, pp.5848~5856 (2009)
- Kim YK, "Functional links between clustered microRNAs:suppression of cell cycle inhibitors by microRNA clusters in gastric cancer", Nucleic Acids Res, 37, pp.1672~1681 (2009)
- Kota J, "Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model", Cell, 137(6), pp.1005~1017 (2009)
- Lee HJ, "MicroRNA expression in ovarian carcinoma and its correlation with clinicopathological features", World Journal of Surgical Oncology, 10, pp.174~184 (2012)
- Loffler D, "Interleukin-6 dependent survival of multiple myeloma cells involves the Stat3- mediatde induction of microRNA-21 through a highly conserved enhancer", blood, 110, pp.1330~1333 (2007)
- Mattie MD, "Optimized high-throughput microRNA expression profiling provides novel biomarker assessment of clinical prostate and breast cancer biopsies", Molecular Cancer, 5, pp.24~ (2006)
- McCarthy DJ, "Testing significance relative to a fold-change threshold is a TREAT", Bioinformatics, 25(6), pp.765~771 (2009)
- Munker R, "MicroRNA profiling in cancer", Clinical Science, 121, pp.141~158 (2011)
- Pichiorri Fl, "MicroRNAs regulate critical genes associated with multiple myeloma pathogenesis", PNAS, 105(35), pp.12885~12890 (2008)
- Porkka KP, "MicroRNA expression profiling in prostate cancer", Cancer Res, 67(13), pp.6130~6135 (2007)
- Roccaro AM, "MicroRNAs 15a and 16 regulate tumor proliferation in multiple myeloma", Blood, 26(113), pp.6669~6680 (2009)
- Jun-ichi Satoh, "Molecular network analysis of human microRNA targetome: from cancers to Alzheimer’s disease", BioData Mining, 5(1), pp.17~ (2012), Springer Nature[DOI]
- Schetter AJ, "MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma", JAMA, 299(4), pp.425~436 (2008)
- 손지웅, "MicroRNA Expression Profiles in Korean Non-Small Cell Lung Cancer", Tuberculosis and Respiratory Diseases, 67(5), pp.413~421 (2009), 대한결핵및호흡기학회
- 성미희, "자궁경부 이형성증과 암의 진단을 위한 액상세포 검체에서 p16INK4a", Ki-67 이중면역염색의 평가, 2015, pp.47(3), 132~139[DOI]
- Takimoto M, "Close relation between 14q32", IGH translocations and chromosome 13 in abnormalities multiple myeloma: a high incidence of 11q13, MAF, pp.International Journal of Hematology, 2008[DOI]
- Tan KS, "Expression profile of microRNAs in young stroke patients", PLoS One, 4(11), pp.e7689~ (2009)
- Vasilatou D, "The role of microRNAs in normal and malignant hematopoiesis", European Journal of Haematology, 84, pp.1~16 (2009)
- Yaguang X, "Systematic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin- embedded samples", RNA, 13, pp.1668~1674 (2007)