Diagnostic Availability of Estrogen Receptor Alpha mRNA on Cervical Cancer Tissue
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Cervical cancer is the fourth most frequently diagnosed cancer in women worldwide. In lower Human Development Index countries, it has the second highest incidence and mortality among cancer in women. Therefore, better diagnosis and treatment systems are needed. Among them, estrogen receptor alpha (ER-α) mRNA expression has been analyzed with RT-qPCR since several studies reported that ER-α is necessary in the maturation of the uterus and is related to cervical cancer. In this study, ER-α quantitative analysis was performed on various lesions and normal tissue samples. Based on the receiver operating characteristic (ROC) curve, its sensitivity and specificity were 85% and 75%, respectively, showing higher or similar results to those of conventional HPV tests. In addition, its expression level was analyzed with clinical information. With regression analysis, the R square value between the ER-α mRNA expression level and menopause status was 0.5041, indicating a strong correlation. This study was performed as part of a pilot study and suggests that ER-α is related to carcinogenesis. Future studies will examine other hormones and menopausal factors with a larger sample size.
Abstract
Cervical cancer is the fourth most frequently diagnosed cancer in women worldwide. In lower Human Development Index countries, it has the second highest incidence and mortality among cancer in women. Therefore, better diagnosis and treatment systems are needed. Among them, estrogen receptor alpha (ER-α) mRNA expression has been analyzed with RT-qPCR since several studies reported that ER-α is necessary in the maturation of the uterus and is related to cervical cancer. In this study, ER-α quantitative analysis was performed on various lesions and normal tissue samples. Based on the receiver operating characteristic (ROC) curve, its sensitivity and specificity were 85% and 75%, respectively, showing higher or similar results to those of conventional HPV tests. In addition, its expression level was analyzed with clinical information. With regression analysis, the R square value between the ER-α mRNA expression level and menopause status was 0.5041, indicating a strong correlation. This study was performed as part of a pilot study and suggests that ER-α is related to carcinogenesis. Future studies will examine other hormones and menopausal factors with a larger sample size.
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