항암제 투여한 골수성백혈병환자의 혈청단백 전기영동상

Abstract

In this present study, we carried out plasma protein electrophoresis by a method for cellulose acetate membrane electrophoresis and sodiumdodecylsulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). Plasmas were isolated from patients with acute myelogenous leukemia (AML) and patients with chronic myelogenous leukemia (CML). The composition and variation of the electrophoreticprotein fractions were compared with those of normal patients. CML and AML patients showed a low concentration of albumin， 2-globulin, 1-globulin and 2-globulin. The concentrations of 2-globulin and -globulin were high in CML plasma but low in AML plasma. 1-globulin in the plasma of CML and AML patients was lower than normal. The results of the SDS-PAGE showed that the plasma isolated from AML and CML patients has a typical protein band (MW. 35 KD) and a thicker protein band (MW. 30 KD) than the protein band of normal patient’s plasma. These results suggested that it is possible to use these results for diagnosis of myelogenous leukemia. In addition, the two bands on SDS-PAGE make it interesting to study the possibility of the mechanisms of myelogenous leukemia.