PCR-RFLP에 의한 대중목욕탕 내 Nontuberculous Mycobacteria의 동정
Identification of Nontuberculous Mycobacteria Existing in Public Bathroom Water by PCR-Restriction Fragment Length Polymorphism
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Abstract
Thirty two of bathroom water samples from public bathroom in Seoul areas were examined using acid-fast staining, Löwenstein-Jensen (L-J) medium culture and PCR-restriction fragment length polymorphism (PCR-RFLP). In 6.25% (2/32) bathroom water samples, acid-fast bacilli were detected by AFB stain, and in 21.9% (7/32) bathroom water samples, acid fast bacilli grew on L-J media. Of them, six acid-fast bacilli were identified as Mycobacterium avium, and the other AFB as Mycobacterium szulgai by PCR-RFLP. These results are suggested that accidental nontuberculosis mycobacterial infection to a weakness person will be possible in public area.
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