충청지역에서 분리된 사람 유래 대장균 및 닭 유래 대장균의 항균제 내성 및 MLST를 이용한 유전형의 분포 조사
Molecular Characterization of Escherichia coli Isolates from Humans and Chickens in Chungcheong Area using MLST Analysis
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초록
최근 항균제의 광범위한 사용으로 인한 항균제 내성세균의 출현과 확산이 축산환경에서 빈번하게 일어나고 있고 이렇게 출현한 다제 내성세균은 사람에게 직접 전파될 수 있어 큰 문제가 되고 있다. 본 연구는 2013년 7월부터 2014년 7월까지 충청지역의 임상검체(n=44) 및 양계농장의 닭(n=34)에서 분리된 78주의 대장균을 대상으로 이루어졌다. PCR과 염기서열 분석을 통하여 MLST 분석과 class 1 integron의 유전형을 분석하였다. 항균제 감수성 검사는 디스크 확산법으로 시행하였다. 사람 유래 대장균의 경우 ST131 (n=20)과 ST38(n=15)이 가장 많았고 닭 유래 대장균은 ST752(n=7)이 가장 많았으며 ST117, ST189, ST69는 각각 4주였다. 사람에서 유래한 대장균은 총 44주 중 25주가 class 1 integron을 가지고 있었다. 반면 닭에서 유래한 대장균은 총 34주 중 11주가 class 1 integron을 가지고 있었다. 충청지역에 분포되어있는 사람유래 대장균과 닭 유래 대장균의 유전형 및 항균제 내성 패턴에 차이를 보였다.
Abstract
Lately, antimicrobial resistant bacteria has emerged and disseminated in livestock environment because of excessive use of antimicrobial agents for therapeutic and growth promotion purposes to food animals. Especially, there is potential for multidrug-resistant bacteria that can be transmitted from animals to man. In this study, we investigated genotypes of E. coli strains isolated from human and chicken using multi-locus sequence typing (MLST) and antimicrobial resistance patterns by disk diffusion method. In addition, integron study involved in antimicrobial resistance mechanisms. From July 2013 to July 2014, E. coli strains isolated from clinical specimens (n = 44) and poultry chickens (n = 34). ST131 (n = 20) was the most common in human-derived E. coli. ST752 (n = 7) was the most common and each ST117, ST189, and ST69 were four isolates in chicken-derived E. coli. 25 of 44 E. coli strains isolated from humans had a class 1 integron while 11 of 34 E. coli strains isolated from chicken had a class 1 integron. There were difference in genotypes and antimicrobial resistance patterns between chicken-derived E. coli and human-derived E. coli.
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