Regulatory T Cells Promote Pancreatic Islet Function and Viability via TGF-β1 in vitro and in vivo
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Regulatory T cells (Treg), known as immune-suppressors, may help modulate the immune response. In this study, we investigated the effect of Treg-derived TGF-β1 on pancreatic islet cell function in vitro and in vivo. One hundred eighty IEQ (islet equivalents) of pancreatic islets, the marginal amount to regulate blood glucose level after syngeneic islet transplantation in mouse type 1 diabetes (T1D) model, were co-cultured with 4×106 Treg cells for 48 hours. The changes in TGF-β1, interleukin-6 (IL-6), and insulin secretion levels were measured and analyzed among the Treg-only group, the islet-only group, and the Treg/islet co-cultured group. In the Treg/islet co-cultured group, IL-6 and insulin secretion levels were increased (P＜ 0.0005, P＜ 0.005) and islet viability was improved (P＜0.005) compared with the islet-only group. Furthermore, after transplantation, the co-cultured islets regulated blood glucose levels efficiently in the T1D mouse model. These data suggest that Treg could improve islet functions and viability via the TGF-β1 secretion pathway (P＜0.05∼0.005), thus the use of Treg in islet transplantation should be explored further.
Abstract
Regulatory T cells (Treg), known as immune-suppressors, may help modulate the immune response. In this study, we investigated the effect of Treg-derived TGF-β1 on pancreatic islet cell function in vitro and in vivo. One hundred eighty IEQ (islet equivalents) of pancreatic islets, the marginal amount to regulate blood glucose level after syngeneic islet transplantation in mouse type 1 diabetes (T1D) model, were co-cultured with 4×106 Treg cells for 48 hours. The changes in TGF-β1, interleukin-6 (IL-6), and insulin secretion levels were measured and analyzed among the Treg-only group, the islet-only group, and the Treg/islet co-cultured group. In the Treg/islet co-cultured group, IL-6 and insulin secretion levels were increased (P＜ 0.0005, P＜ 0.005) and islet viability was improved (P＜0.005) compared with the islet-only group. Furthermore, after transplantation, the co-cultured islets regulated blood glucose levels efficiently in the T1D mouse model. These data suggest that Treg could improve islet functions and viability via the TGF-β1 secretion pathway (P＜0.05∼0.005), thus the use of Treg in islet transplantation should be explored further.
참고문헌 (28)
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