공기압 차이를 이용한 무방부제 다회용 점적기의 세균 억제 효과
The Antibacterial Effect of a Preservative-free Multi-dose Eye Dropper Using Air Pressure Differentials
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초록
본 연구의 목적은 기존 점적기 모델의 단점을 보완하여 새롭게 설계된 공기압 차이를 이용한 무방부제 다회용 점적기의 세균 억제 효과를 평가하는 것이다. 성능을 평가하기 위해 상용 다회용 점적기, 기존 다회용 무방부제 점적기, 공기압 차이를 이용한 무방부제 점적기의 세균 오염 및 여과 효과를 시간 경과에 따라 비교하였다. 세균 오염을 평가하기 위해 대한민국약전에서 제시한 세균 종에 따라 Staphylococcus aureus, Bacillus subtilis, Aspergillus brasiliensis를 사용하였으며, 맥팔랜드(McFarland) 혼탁도 기준에 따라 세균 오염 정도를 측정하였다. 사용 기간에 따른 오염 정도를 평가한 실험에서 일반적인 다회용 점적기의 경우, 사용 기간이 길어질수록 세균 오염이 크게 증가한 반면, 공기압 차이를 이용한 무방부제 점적기에서는 실험 기간 동안 세균 성장이 전혀 관찰되지 않았다. 정방향 및 역방향 여과 실험에서도 새로운 공기압 차이를 이용한 다회용 무방부제 점적기는 세균이 점적기 안으로 침투하거나 점적기 끝을 통해 외부로 배출되는 것을 효과적으로 차단하였다. 결론적으로, 공기압 차이를 이용한 무방부제 다회용 점적기는 세균 침입을 효과적으로 차단하였으며, 방부제 없이도 점안액의 무균 상태를 보장할 수 있는 대안으로써 활용될 수 있을 것으로 기대한다.
Abstract
This study aimed to evaluate the antibacterial effects of a newly designed preservative-free multi-dose eye dropper that addresses various shortcomings found in conventional models. To thoroughly assess its performance, we compared bacterial contamination and filtration effects over time among three types of droppers: commercially available multi-dose droppers, conventional preservative-free droppers, and the newly designed preservative-free dropper utilizing air pressure differentials. The bacterial strains used to evaluate contamination included Staphylococcus aureus, Bacillus subtilis, and Aspergillus brasiliensis, with turbidity levels measured according to the McFarland Standards to ensure accuracy. In typical multi-dose eye droppers, bacterial contamination tended to increase significantly with extended usage periods, leading to potential health risks. However, in the preservative-free eye dropper using air pressure differentials, no bacterial growth was observed throughout the entire experimental period. In both forward and reverse filtration tests, the new preservative-free eye dropper successfully prevented bacteria from entering the vial and from being released to the outside through the dropper tip. Overall, this preservative-free reusable eye dropper, which relies on air pressure differentials, offers a safe, effective, and hygienic option for administering eye drops, while minimizing the side effects associated with preservatives and maintaining the sterility of the solution over time.
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