Caffeic Acid Phenethyl Ester Inhibits Cell Proliferation and Induces Apoptosis in Human Ovarian Cancer Cells
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The phenethyl ester of caffeic acid (CAPE), an active component of honeybee propolis extract, is shown to inhibit cancer growth previously. However, studies on human ovarian cancer are largely obscure. This study evaluated the effects of CAPE as a potential anti-proliferative and pro-apoptotic agent in the human ovarian cancer line, OVCAR-3. CAPE treated OVCAR-3 cells showed inhibition of cell viability and proliferation in a dose-dependent manner by WST-1 assay,LDH assay and bromodeoxyuridine (BrdU) incorporation assay. Furthermore, CAPE-mediated OVCAR-3 cell growth inhibition was associated with apoptotic changes as evident by cell cycle arrest and accumulation of cells in the apoptotic phase and DNA fragmentation. Taken together,CAPE inhibits cell proliferation via DNA synthesis reduction and induces apoptotic cell death via DNA damage, thus elucidating a novel, plausible mechanism of CAPE anti-tumorigenic property in OVCAR-3 cells.
Abstract
The phenethyl ester of caffeic acid (CAPE), an active component of honeybee propolis extract, is shown to inhibit cancer growth previously. However, studies on human ovarian cancer are largely obscure. This study evaluated the effects of CAPE as a potential anti-proliferative and pro-apoptotic agent in the human ovarian cancer line, OVCAR-3. CAPE treated OVCAR-3 cells showed inhibition of cell viability and proliferation in a dose-dependent manner by WST-1 assay,LDH assay and bromodeoxyuridine (BrdU) incorporation assay. Furthermore, CAPE-mediated OVCAR-3 cell growth inhibition was associated with apoptotic changes as evident by cell cycle arrest and accumulation of cells in the apoptotic phase and DNA fragmentation. Taken together,CAPE inhibits cell proliferation via DNA synthesis reduction and induces apoptotic cell death via DNA damage, thus elucidating a novel, plausible mechanism of CAPE anti-tumorigenic property in OVCAR-3 cells.
참고문헌 (19)
- Demestre M, "CAPE (caffeic acid phenethyl ester)-based propolis extract (Bio 30) suppresses the growth of human neurofibromatosis (NF) tumor xenografts in mice", Phytother. Res, 23, pp.226~230 (2009)
- Dundr P, "Ovarian carcinoma: current diagnostic principles", Cesk. Patol, 46, pp.53~61 (2010)
- Fruehauf JP, "Reactive oxygen species: a breath of life or death?", Clin. Cancer. Res, 13, pp.789~794 (2007)
- Grunberger D, "Preferential cytotoxicity on tumor cells by caffeic acid phenethyl ester isolated from propolis", Experientia, 44, pp.230~232 (1988)
- Hishikawa K, "Oral flavonoid supplementation attenuates atherosclerosis development in apolipoprotein E-deficient mice", Arterioscler Thromb. Vasc. Biol, 25, pp.442~446 (2005)
- Ho CC, "Effects of CAPE-like compounds on HIV replication in vitro and modulation of cytokines in vivo", J. Antimicrob. Chemother, 56, pp.372~379 (2005)
- Ho HC, "Caffeic acid phenethyl ester inhibits arterial smooth muscle cell proliferation and migration in vitro and in vivo using a local delivery system", Cell Mol. Biol. (Noisy-le-grand), (55), pp.OL1161~OL1167 (2009)
- Kamatou GP, "South African Salvia species: a review of biological activities and phytochemistry", J. Ethnopharmacol, 119, pp.664~672 (2008)
- Kandalaft LE, "Immunotherapy for Ovarian Cancer: What’s Next?", J. Clin. Oncol. (2010)
- Lee KJ, "Protective effect of caffeic acid phenethyl ester on tert-butyl hydroperoxide-induced oxidative hepatotoxicity and DNA damage", Food Chem. Toxicol, 46, pp.2445~2450 (2008)
- Lee KJ, "Protective effect of saponins derived from the roots of Platycodon grandiflorum against carbon tetrachloride induced hepatotoxicity in mice", Food Chem. Toxicol, 46, pp.1778~1785 (2008)
- Lee Y, "Caffeic acid phenethyl ester-mediated Nrf2 activation and IkappaB kinase inhibition are involved in NFkappaB inhibitory effect: structural analysis for NFkappaB inhibition", Eur. J. Pharmacol, 643, pp.21~28 (2010)
- Nagaoka T, "Caffeic acid phenethyl ester (CAPE) analogues: potent nitric oxide inhibitors from the Netherlands propolis", Biol. Pharm. Bull, 26, pp.487~491 (2003)
- Orban Z, "Caffeic acid phenethyl ester induces leukocyte apoptosis, modulates nuclear factor-kappa B and suppresses acute inflammation", Neuroimmunomodulation, 7, pp.99~105 (2000)
- Shukla S, "Molecular targets for apigenininduced cell cycle arrest and apoptosis in prostate cancer cell xenograft", Mol. Cancer Ther, 5, pp.843~852 (2006)
- Syed DN, "Dietary agents for chemoprevention of prostate cancer", Cancer Lett, 265, pp.167~176 (2008)
- Szliszka E, "Ethanolic Extract of Propolis Augments TRAIL-Induced Apoptotic Death in Prostate Cancer Cells", Based Complement Alternat. (2009)
- Wang T, "DNA damage induced by caffeic acid phenyl ester in the presence of Cu (II) ions: potential mechanism of its anticancer properties", Cancer Lett, 263, pp.77~88 (2008)
- Zhang M, "Green tea consumption enhances survival of epithelial ovarian cancer", Int. J. Cancer, 112, pp.465~469 (2004)