메탄발효 처리된 가축분뇨내의 내분비계 장애물질에 대한 조사 연구
A Survey on Endocrine Disrupting Chemicals in Animal Wastes Treated with Methane Fermentation
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인간과 가축에서 발생된 내분비계 장애물질들이 자연계로 유입되고, 매우 낮은 농도에서도 토착 동물군에 교란 작용을 유발시킬 수 있다는 잠재적 오염 가능성에 대한 우려가 증가되고 있다. 그러나 현재까지 가축분뇨 중의 내분비계 장애물질에 대한 연구는 거의 이루어지지 않았다. 따라서 본 연구는 이러한 장애물질 중 여성 호르몬으로 알려진 17베타-에스트라디올, 에스트론, 에스트리올을 분석하여 분뇨 중 에스트로젠 농도에 대한 기초자료를 제공하고자 수행되었다. 공시시료는 생물생태센터의 분뇨 저장조, 메탄 소화조, 오니 제거조에서 수집하여 가스크로마토그래피 질량분석기를 이용하여 에스트로젠 농도를 분석하였다. 또한 에스트로젠 대 비료성분 질량비를 알아보기 위해 질소와 인산도 측정하였다. 분석 결과 가축분뇨 중에는 다량의 에스트로젠 호르몬이 존재하고 있었으며, 분뇨 저장조에서 98.7 μg/L로 가장 높은 농도를 보인 반면 오니 제거 소화액에서 가장 낮은 농도인 3.4 μg/L로 조사되었다. 분뇨 저장조에서 채취된 시료의 17베타-에스트라디올과 에스트론의 평균농도는 6.8 μg/L와 68.7 μg/L 였고, 오니 제거조는 각각 2.6 μg/L 및 1.9 μg/L로 분석되었다. 반면 에스트리올은 모든 시료에서 검출되지 않았다. 본 연구 조사는 가축분뇨내의 에스트로젠 농도에 대한 기초자료를 제공할 수 있으나 에스트로젠 호르몬의 저감 방안에 대한 기작을 설명하기에는 한계가 있다. 따라서 축종별로 적용되고 있는 분뇨관리 및 축사시설에 대해 시공간적 변이를 고려할 수 있는 광범위한 조사 연구와 더불어 여러가지 처리방법에 의해 농경지에 살포되고 있는 분뇨내의 에스트로젠 농도와 조성 및 생물학적 활성도에 대한 추가연구가 필요하다.
Abstract
Concerns about endocrine disrupting chemicals emitted from humans and animals have been increased because these compounds are detected at very low levels in environment and adversely affect on indigenous fauna. To date, there is little information regarding the concentration of these compounds in animal wastes. In this study, the female hormones, 17β-estradiol (E2), estrone (E1) and estriol, were measured to provide baseline data in animal wastes. Samples were collected from animal waste storage, methane digester and sludge separated wastewater and analyzed by gas chromatography-mass spectrometry. To measure the mass ratios of estrogen to macronutrients, nitrogen and phosphorous were also determined. Sample collected from animal waste storage had the highest estrogen concentration (98.7 μg/L), while sludge separated wastewater had the lowest concentration (3.4 μg/L). The mean concentrations of E2 and E1 in waste storage sample were 6.8 μg/L and 68.7 μg/L, respectively. In sludge separated wastewater, the mean concentration of both E2 and E1 were reduced to 2.6 μg/L and 1.9 μg/L, respectively. However, estriol was not detected in any of the samples collected. Mean ratios of E2 and E1 to macronutrients were significantly different between the methane wastewater and sludge separated wastewater owing to elimination of solid particles.
참고문헌 (26)
- "Global assessment of the state-of-the- science of endocrine disruptors" (2002), WHO
- "An integrated assessment of estrogenic contamination and biological effects in the aquatic environment of The Netherlands", 59, pp.511~524 (2005)
- "A survey of endocrine disrupting chemicals (EDCs) in municipal sewage and animal waste effluents in the Waikato region of New Zealand", 355, pp.135~144 (2006)
- "Overview of endocrine disruptor research activity in the United States", 39, pp.1227~1236 (1999)
- "Overall review on endocrine disruptors", 6(3), pp.135~156 (2002)
- "Naturally produced steroid hormones and their release into the environment", 75, pp.1859~1871 (2003)
- "The role of estradiol and estrogen in chicken manure silage in hyperestrogenism in cattle", 65(2), pp.68~68 (1998)
- "Deteriorating trends in male reproduction; idiopathic of environmental?", 60, pp.121~130 (2000)
- "Induction of phytoestrogen production in Medicago sativa leaves by irrigation with sewage water", 35(3), pp.363~369 (1995)
- "Pharmaceuticals, hormones, and other organic wastewater contaminants in US streams, 1999-2000: a national reconnaissance", 36, pp.1202~1211 (2002)
- estradiol-17-beta after intramuscular injection into Holstein steer calves, , 1996, 681~690
- "Excretion of infused C-14-estroid hormones via faeces and urine in domestic livestock", 43, pp.43~63 (1996)
- "Manure-borne estrogens as potential environmental contaminants: A review", 37, pp.5471~5478 (2003)
- "Sex hormones originating from different livestock production systems fate and potential disrupting activity in the environment", 473, pp.27~37 (2002)
- "Estrogen content of dairy and swine wastes", 38, pp.3567~3573 (2004)
- "Quantification of estrogens in ground water and swine lagoon samples using solid- phase extraction, pentafluorobenzyl", trimethylsilyl derivatizations and gas chromatography-negative ion chemical ionization tandem mass spectrometry, 2003, pp.1017, 167~185
- "Standard methods for the examination of water and wastewater" (1995), American Public Health Association, American Water Works Association, and Water Environment Federation: Washington, DC
- "SAS user's guide : Statistics" (1998), SAS Inst., Inc., Gary, NC., USA
- "Dairy wastewater, aquaculture, and spawning fish as source of steroid hormones in the aquatic environment", 38(23), pp.6377~6384 (2004)
- "Effects of estrone and 17beta-estradiol on vegetative growth of medicago-sativa" (1992)
- "Identification of estrogenic chemicals in STW effluent In vivo responses in trout and roach", 32, pp.1559~1565 (1998)
- "Behavior and occurrence of estrogens in municipal sewage treatment plants-Ⅰ", 225, pp.81~90 (1999)
- "Fate of natural estrogen conjugates in municipal sewage transport and treatment facilities", 301, pp.199~209 (2003)
- "Comment on ＂Pharmaceuticals, hormones, and other organic wastewater contaminants in US streams, 1999-2000: A national reconnaissance", 36, pp.4005~4006 (2002)
- "17 beta-estradiol as an indicator of animal waste contamination in mantled karst aquifers", 29(3), pp.826~834 (2000)
- "17 beta-estradiol and testosterone in soil and runoff from grasslands amended with broiler letter", 29(5), pp.1604~1611 (2000)