수온별 붉바리(Epinephelus akaara)의 행동, 생존율 및 혈액생리학적 반응
Behavior, Survival and Blood Physiological Responses of Red-spotted Grouper Epinephelus akaara, at Different Water Temperature
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
본 연구에서는 붉바리 (Epinephelus akaara)의 장거리 수송 시 최적의 수온 (대사활동과 스트레스를 최소화하는 수 온)을 조사하고자, 수온별 (9, 12, 15, 18 및 21℃)로 48시간 동안 어체를 노출시켜 어류의 행동 변화, 생존 및 혈액생리학적 반응을 확인하였다. 9℃에 노출된 붉바리는 48시간만 에 전량 폐사하였지만, 12 15, 18 및 21℃의 그룹에서는 모두 생존하였다. 15, 18 및 21℃의 붉바리는 정상적인 유영 활동을 보였으나, 12℃에 노출시킨 붉바리는 유영없이 수조 바닥에 가라앉아 있는 상태를 보였다. 혈장 코티졸 농도와 글루코스는 다른 실험구보다 12℃에 노출시킨 붉바리 에서 더 유의하게 높았다 (P<0.05). 12 및 15℃ 그룹은 18 및 21℃보다 Ht 및 Hb가 유의한 차이를 보였다 (P<0.05). AST, NH3 및 삼투질 농도는 혈장 코티졸과 비슷한 양상을 보였으나 (P<0.05), ALT와 총단백질은 다른 실험구에 비해 유의적인 차이를 보이지 않았다 (P>0.05). 이러한 결과로 미루어 볼 때, 붉바리의 장거리 수송을 위한 적정 수온은 15℃ 임을 시사하고 있다.
Abstract
To determine the optimum water temperature (a temperature which minimizes metabolic activities and stress in fish) for long distance transportation of red-spotted grouper (Epinephelus akaara), by evaluating the behavior, survival and physiological and hematological responses to variable water temperature conditions (9, 12, 15, 18 and 21℃) for 48 hours. Fish exposed to 9℃ died at 48 hours exposure, but those exposed to 12, 15, 18 and 21℃ treatment groups all survived. Fish in the 15, 18 and 21℃ exposure groups exhibited normal swimming, while those exposed to 12℃ were observed to be stationary at the bottom of the tank. The plasma cortisol and glucose concentration were higher in fish exposed to 12℃ than at other temperature conditions. The fish in the 12 and 15℃ groups had significantly higher hematocrit (Ht) and hemoglobin (Hb) than those in the 18 and 21℃ groups (P<0.05). Levels of aspartate aminotransferase (AST), NH3 and osmolality showed a pattern similar to the levels of plasma cortisol, while alanine aminotransferase (ALT) and total protein did not significantly vary among the experimental groups. These results indicate that the optimum water temperature for long distance transportation of the red-spotted grouper is 15℃.
참고문헌 (32)
- Benli AÇK, "Sublethal ammonia exposure of nile tilapia (Oreochromis niloticus L.): Effects on gill, liver and kidney histology", Chemosphere, 72, pp.1355~1358 (2008)
- Barton BA, "Metabolic cost of acute physical stress in juvenile steelhead", Trans. Am. Fish. Soc, 116, pp.257~263 (1987)
- Barton BA, "Physiological change in fish from stress in aquaculture with emphasis in the response and effects of corticosteroids", Annu. Rev. Fish. Dis, 1, pp.3~26 (1991)
- Byrne P, "Effects of a cationic detergent on the gills and blood chemistry of rainbow trout (Salmo gairdeneri)", Dis. Aquat. Org, 6, pp.195~196 (1989)
- Cataldi E, "Serum parameters of Adriatic sturgeon Acioenser naccarii (Pisces: Acipenseriformes): effects of temperature and stress", Comp. Bioche. Physiol, 121, pp.351~354 (1998)
- Chang YJ, "Physiological responses of grey mullet (Mugil cephalus) and Nile tilapia (Oreochromis niloticus) by rapid changes in salinity of rearing water", J. Kor. Fish. Soc, 32, pp.313~316 (1999)
- 최철영, "담수사육 감성돔, Acanthopagrus schlegeli의 수온 상승에 따른 HSP90, HSP70 mRNA의 발현 및 혈액 생리학적 변화", 한국양식학회지, 19(4), pp.315~322 (2006), 한국양식학회
- 도용현, "고수온에서 조피볼락(Sebastes schlegeli)의 산소소비 및 혈액성상 변화", 수산해양교육연구, 28(3), pp.738~745 (2016), 한국수산해양교육학회
- Dosdat A, "Effect of chronic exposure to ammonia on growth, food utilization and metabolism of the European sea bass (Dicentrarchus labrax)", Aquat. Living. Resour, 16, pp.509~520 (2003)
- Ishioka H, "Stress reactions induced by environmental salinity changes in red-seabream", Bull. Jap. Soc. Sci. Fish, 46, pp.523~532 (1980)
- 김영수, "염분변화 속도를 달리한 담수순화 과정에서 강도다리 Platichthys stellatus의 생리학적 반응", 한국양식학회지, 22(1), pp.28~33 (2009), 한국양식학회
- 민병화, "담수 및 해수사육 감성돔, Acanthopagrus schlegeli의 생리활성과 성장에 미치는 갑상선 호르몬의 효과", 한국양식학회지, 19(3), pp.149~156 (2006), 한국양식학회
- Mommsen TP, "Cortisol in teleosts: dynamics, mechanisms of action, and metabolic regulation", Rev. Fish Biol. Fish, 9, pp.211~268 (1999)
- Nakagawa, H., "Electrophoretic evidence of seasonal variation of carp plasma albumin", J. Fac. Fish. Anim. Husb. Hiroshima Univ, 16, pp.99~106 (1977)
- Olsen JE, "A localized decrease of GA1 in shoot tips of Salix pentandra seedings precedes cessation of shoot elongation under short photoperiod", Physiol. Plant, 95, pp.627~632 (1995)
- Ozaki H, "Physiology of fish, Vol. 1, Blood. Circulation", pp.1~326 (1978), Midori-shobo
- Pan CH, "The resistance to ammonia stress of Penaeus monodon Fabricius juvenile fed diets supplemented with astaxanthin", J. Exp. Mar. Boil. Ecol, 297, pp.107~118 (2003)
- 박종연, "저수온 스트레스에 따른 능성어 (Epinephelus septemfasciatus) 혈액의 생리생화학적 반응", 한국어류학회지, 28(1), pp.1~8 (2016), 한국어류학회
- Perry SF, "β-adrenergic signal transportation in fish: interactive effects of catecholamines and cortisol", Fish Physiol. Bioche, 11, pp.195~203 (1993)
- Person-Le Ruyet J, "Effects of repeated hypoxic shocks on growth and metabolism of turbot juveniles", Aquat. Living Resour, 16, pp.25~34 (2003)
- Randall DJ, "Ammonia toxity in fish", Mar. Pollut. Bull, 45, pp.17~23 (2002)
- Raune NM, "Moulation of the response of rainbow trout (Onchorhynchus mykiss Walbaum) to confirnement, by an ectoparasitic (Argulus foliaceus L.) infestation and cortisol feeding", Fish Physiol. Bioche, 20, pp.43~51 (1999)
- Robert ER, "Biological indicators of stress in fish", American Fish. Trans. Am. Fish. Soc, 121, pp.274~276 (1992)
- Robertson LC, "Effects of lesions of temporal-parietal junction on perceptual and attentional processing in humans", J. Neuro, 8, pp.3757~3769 (1988)
- Santos MA, "Anguilla anguilla L. Stress biomarkers recovery in clean water and secondary treated pulp mill effluent", Ecotoxico. Environ. Safe, 35, pp.96~100 (1996)
- Schreck CB, "Stress and rearing of salmonids", Aquaculture, 28, pp.241~249 (1982)
- Vijayan V, "Developing human biliary system in three dimensions", Develop. Biol, 249, pp.389~398 (1997)
- Vinodhini R, "The impact of toxic heavy metal son the hematological parametersin common carp (Cyprinuscarpio L.)", Iranian J. Environ. Health Sci. Eng, 6, pp.23~28 (2009)
- Wendelaar Bonga SE, "The stress response in fish", Physiol. Rev, 77, pp.591~625 (1997)
- Wright PA, "Ionic, osmotic, and nitrogenous waste regulation", Fish Physiol, 26, pp.283~318 (2007)
- Yanagisawa, T., "Plasma albumins in elasmobranchs", Nissuishi, 50, pp.1083~ (1984)
- 양성진, "조피볼락(Sebastes schlegeli)의 장거리 수송을 위한 적정 수온 및 염분 조건 탐색", 한국수산과학회지, 50(1), pp.25~31 (2017), 한국수산과학회