Microcosm 실험을 이용한 생물유래 살조물질 Naphthoquinone 유도체의 유해 남조류 제어효과 및 기존물질과의 우수성 비교
Superiority comparison of biologically derived algicidal substances (naphthoquinone derivative) with other optional agents using microcosm experiments
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초록
유해 남조류 Microcystis 종에 의한 녹조현상은 매년 빈번하게 일어나며, 이로 인한 수자원의 질적 변화와 먹는 물확보에 문제가 발생하고 있다. Microcystis 종에 의한 피해를 막고자 개발된 naphthoquinone (NQ) 유도체 물질의 장점 분석을 위해 국내에서 빈번히 사용되는 황토를 이용한 microcosm 실험을 하고자 하였다. 그 결과, NQ 40, NQ 2-0 물질은 선행 연구 결과와 동일하게 유해 남조류 Microcystis 종을 선택적으로 99.9%, 99.6% 제어했으며, 식물플랑크톤 종 다양성을 증진시켰다. 그러나, 황토를 처리한 실험구는 실험 초기 일시적인 조류 제어효과를 보인 후 다시 증가하였으며, 유용 조류를 포함한 모든 식물플랑크톤에게 적용되어 다른 조류의 성장은 이루어지지 않았다. 뿐만 아니라, NQ 물질을 처리한 처리구는 비생물학적, 생물학적요인 모두 영향을 미치지 않았으며, 대조구와 유사한 경향이 관찰되었다. 따라서, 최종적으로 개량된 유해 남조류 제어물질 NQ 2-0은 높은 살조효과, 선택적 제어효과, 저독성, 자연분해에 의한 비잔류성 뿐만 아니라, 편의성 및 경제성까지 갖춘 새로운 살조물질로서 현재까지 개발된 살조물질보다 현장 생태계 적용에 가장 적합한 친환경 녹조제어 물질이라고 판단된다.
Abstract
Bloom-forming toxic cyanobacteria Microcystis spp. are common in the summer season in temperate freshwater ecosystems. Often, it leads to the degradation of water quality and affects the quality of drinking water. In a previous study, NQ (naphthoquinone) compounds were shown to be effective, selective, and ecologically safe algicides for Microcystis spp. blooms. To analyze the superiority of developed NQ derivatives, we conducted a microcosm experiment using clay, which is frequently used in South Korea. Similar to previous studies, the NQ 40 and NQ 2-0 compounds showed high algicidal activities of 99.9% and 99.6%, respectively, on Microcystis spp. at low concentrations (≥1 μM) and enhanced phytoplankton species diversity. However, when treated with clay, a temporary algicidal effect was seen at the beginning of the experiment that gradually increased at the end. In addition, treatment with the NQ compounds did not affect either the abiotic or biological factors, and similar trends were observed with the control. These results showed that the NQ 2-0 compound was more effective, with no ecosystem disturbance, and more economical than the currently used clay. These results suggest that NQ 2-0 compound could be a selective, economically and ecologically safe algicide to mitigate harmful cyanobacterial blooms in the field.
참고문헌 (40)
- Standard Methods for the Examination of Water and Wastewater, "APHA", APHA-AWWA-WEF, ~ (2005)
- Climate Change and Water, "Bates B", Intergovernmental Panel on Climate Change Secretariat, ~ (2008)
- Biggins J, "Evaluation of selected benzoquinones, naphthoquinones, and anthraquinones as replacements for phylloquinone in the A1 acceptor site of the photosystem I reaction center", Biochemistry, 29, pp.7259~7264 (1990)
- Binder RG, "Eight 1, 4-naphthoquinones from Juglans", Phytochemistry, 28, pp.2799~2801 (1989)
- 변대흥, "유해 조류 제어를 위한 naphthoquinone 유도체 합성", 석사, 신재생에너지융합학과, pp.2013
- Crouch IJ, "Studies on the isolation of plumbagin from in vitro and in vivo grown Drosera species", Plant Cell Tiss. Org., 21, pp.79~82 (1990)
- Dong Y, "Intratumoral delivery of β-lapachone via polymer implants for prostate cancer therapy", Clin. Cancer Res., 15, pp.131~139 (2009)
- Foflonker F, "Biological methods to control common algal bloom-forming species", Basic Biotechnol. eJournal, 5, pp.19~24 (2009)
- Gumbo RJ, "Biological control of Microcystis dominated harmful algal blooms", Afr. J. Biotechnol., 7, pp.4765~4773 (2008)
- Gumbo JR, "The isolation and identification of predatory bacteria from a Microcystis algal bloom", Afr. J. Biotechnol., 9, pp.663~671 (2010)
- 한상일, "항균 펩타이드를 이용한 녹조현상 원인종 Microcystis aeruginosa의 제어", 환경생물, 36(4), pp.601~609 (2018), 한국환경생물학회[DOI]
- Hickey CW, "Lake sediment phosphorus release management-decision support and risk assessment framework", New Zeal. J. Mar. Fresh., 43, pp.819~856 (2009)
- Jewess PJ, "Herbicidal action of 2 -hydroxy-3-alkyl -1, 4-naphthoquinones", Pest Manag. Sci., 58, pp.234~242 (2002)
- Joo JH, "A field application feasibility assessment of naphthoquinone derivatives for the mitigation of freshwater diatom Stephanodiscus blooms", J. Appl. Phycol., 28, pp.1735~1746 (2016)
- Joo JH, "Ecological assessment of an algaecidal naphthoquinone derivate for the mitigation of Stephanodiscus within a mesocosm", Environ. Pollut., 229, pp.735~745 (2017)
- Joo JH, "Improvement of cyanobacterial-killing biologically derived substances (BDSs) using an ecologically safe and cost-effective naphthoquinone derivative", Ecotox. Environ. Safe., 141, pp.188~198 (2017)
- Kim HG, "Ecology of Harmful Algae", pp.327~338 (2006), Springer
- Koss AM, "Alternative prey disrupt biocontrol by a guild of generalist predators", Biol. Control., 32, pp.243~251 (2005)
- 이창수, "담수수계에서 남조류 증식억제의 기술적, 전략적 접근", 환경생물, 31(4), pp.233~242 (2013), 한국환경생물학회
- Lee HW, "Cyanobacteria-specific algicidal mechanism of bioinspired naphthoquinone derivative, NQ 2-0", Sci. Rep., 8, pp.11595~ (2018)
- 임병진, "식물체를 이용한 조류제어 현장 적용성 실험", 생태와 환경, 35(2), pp.123~132 (2002), 한국수생태학회
- Lin LC, "Cytotoxic naphthoquinones and plumbagic acid glucosides from Plumbago zeylanica", Phytochemistry, 62, pp.619~622 (2003)
- Lürling M, "Case study on the efficacy of a lanthanum-enriched clay (Phoslock®) in controlling eutrophication in Lake Het Groene Eiland (The Netherlands)", Hydrobiologia, 710, pp.253~263 (2013)
- Margalef R, "Information theory in ecology", Gen. Syst, 3, pp.36~71 (1958)
- Monks TJ, "Quinone chemistry and toxicity", Toxicol. Appl. Pharm., 112, pp.2~16 (1992)
- O’Brien PJ, "Molecular mechanisms of quinone cytotoxicity", Chem-Biol. Interact, 80, pp.1~41 (1991)
- Oettmeier W, "QSAR of 1, 4-naphthoquinones as inhibitors of photosystem II electron transport", Quant. Struct. Act. Relat., 5, pp.50~54 (1986)
- Patterson DJ, "Free -Living Freshwater Protozoa" (2003), ASM Press
- 박혜진, "황토의 Microcystis spp. 제거효율 및 생태독성평가", 한국물환경학회지, 30(3), pp.261~268 (2014), 한국물환경학회
- Pielou EC, "Shannon’s formula as a measure of specific diversity: its use and misuse", Am. Nat., 100, pp.463~465 (1969)
- Round FE, "The Diatom Biology and Morphology of Genera" (1990), Cambridge University Press
- 유건상, "여러 흡착제를 이용한 총질소와 총인 흡착특성에 관한 비교연구", 대한화학회지, 56(6), pp.700~705 (2012), 대한화학회
- Schrader KK, "Novel derivate s of 9, 10-anthraquinone are selective algicides against the mustyodor cyanobacterium Oscillatoria perornata", Appl. Environ. Microbiol, 69, pp.5319~5327 (2003)
- Sengco MR, "Controlling harmful algal blooms through clay flocculation 1", J. Eukaryot. Microbiol., 51, pp.169~172 (2004)
- Senhorst HAJ, "Climate change and effects on water quality: a first impression", Water Sci. Technol., 51, pp.53~59 (2005)
- Shao J, "Potential for control of harmful cyanobacterial blooms using biologically derived substances: Problems and prospects", J. Environ. Manage., 125, pp.149~155 (2013)
- Shukla S, "The naphthoquinones, vitamin K3 and its structural analogue plumbagin, are substrates of the multidrug resistance-linked ATP binding cassette drug transporter ABCG2", Mol. Cancer Ther., 6, pp.3279~3286 (2007)
- Sigee DC, "The Ecological Bases for Lake and Reservoir Management", pp.161~172 (1999), Springer
- Thackeray SJ, "Long-term change in the phenology of spring phytoplankton: species-specific responses to nutrient enrichment and climatic change", J. Ecol., 96, pp.523~535 (2008)
- Yamamoto M, "Bacterial flora of the biofilm Formed on the submerged surface of the reed Phragmites australis", Microbes Environ, 20, pp.14~24 (2005)