유동세포분석에 의한 참전복 (Haliotis discus hannai) 세포내 DNA 함량 분석 최적화
A flowcytometric determination of DNA content in Pacific abalone, Haliotis discus hannai cell
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
DNA 함량 조사는 진화의 유전적 기작을 예측하여 종분화를 파악하게 한다. 본 연구의 목적은 참전복 (Haliotis discus hannai)의 DNA 함량을 측정하고 유세포분석기 (flowcytometry)로 고정 (fixation) 없이 DNA 함량 측정에 최적인 조직들을 파악하는 것이다. DNA 함량 (pg nucleus-1)에 있어, 원생생물에 오염된 아가미조직 (2.5±0.08)은 3.2±0.02 pg nucleus-1의 DNA 함량을 보인 근육조직과 외투막조직에 비하여 유의하게 낮았고 (p<0.05) standard reference보다도 낮은 반면, 근육조직과 외투막조직은 standard reference보다 높았다. 본 연구 결과들을 고려 시, 참전복에서 고정 없이 유동세포분석에 적절한 조직은 근육과 외투막이며 본 연구에 적용된 고정 과정이 없는 유세포분석법은 참전복 DNA 함량 분석 시 정확하고, 신속한 방법임이 판명되었다.
Abstract
The level at which analyses of DNA content might contribute more significantly to the genetic mechanisms of evolution lies in the events of speciation. The object of this study was to investigate the DNA content of abalone (Haliotis discus hannai) and determine the optimal tissue samples for measuring the DNA content of abalone by flowcytometry without fixation. The DNA content (pg/nucleus) of gill tissue (2.5±0.08), which was contaminated with protozoa, was significantly lower than that of muscle tissue (3.2±0.02), mantle tissue (3.2±0.02) (p<0.05), and a standard reference standard, while the DNA contents of muscle tissue and mantle tissue were higher than that of the standard reference. Considering the results of this study, DNA content analysis with flowcytometry is an acute and rapid method by which muscle tissue and mantle tissue are the most appropriate sample for measuring the DNA content of abalone without fixation.
참고문헌 (18)
- 안혜석, "Induction of a New Hybrid between Haliotis gigantea Gmelin (♀) and H. discus discus Reeve (♂)", Genes & Genomics, 29(2), pp.239~244 (2007), 한국유전학회
- Ashton-Alcox KA, "Recent Advances in Marine Biotechnology, Vol. 5", pp.85~124 (2001), Science Publishers
- 조준현, "양식 참전복(Haliotis discus hannai) 열성패 추출물의 제조 및 품질특성", 한국수산과학회지, 51(6), pp.640~646 (2018), 한국수산과학회[DOI]
- Da Silva PM, "Comparison of microscopic techniques for the diagnosis of the infection of the European flat oyster Ostrea edulis by the protozoan Bonamia ostreae", J. Invertebr. Pathol., 85, pp.97~104 (2004)
- Estevam J, "Validation of a flow cytometry based G2M delay cell cycle assay for use in evaluating the pharmacodynamic response to Aurora A inhibition", J. Immunol. Methods, 363, pp.135~142 (2011)
- Abalone production continues to grow, coupled with continuing demand, price high and stable. GLOBEFISH-Information and Analysis on World Fish Trade, "FAO", Food and Agriculture Organization of the United Nations, ~ (2017)
- González-Tizón A, "DNA contents, karyotypes and chromosomal location of 18S-5.8S-28S ribosomal loci in some species of bivalve molluscs from the Pacific Canadian coast", Genome, 43, pp.1065~1072 (2000)
- Hinegardner R, "Cellular DNA content of the mollusca", Comp. Biochem. Physiol., 47, pp.447~460 (1974)
- 지영주, "북방전복, Haliotis discus hannai 3배체의 세포유전학적 연구", The Korean Journal of Malacology, 28(1), pp.37~43 (2012), 한국패류학회
- 김희성, "배합사료내 규조류 우점인 미세조류 오일 추출물 첨가가 까막전복(Haliotis discus)의 성장, 체조성 및 패각 색채에 미치는 영향", 한국수산과학회지, 50(6), pp.738~744 (2017), 한국수산과학회[DOI]
- NFFC, "Marine Products in Korea. National Federation of Fisheries Cooperatives" (2000), Suhyup Publ. Co.
- 박인석, "해산송사리 (Oryzias dancena) 2배체와 유도 3배체 조직의 세포주기 비교", 환경생물, 37(4), pp.735~740 (2019), 한국환경생물학회[DOI]
- 박인석, "Morphometric characteristics of diploid and triploid Far Eastern catfish, Silurus asotus", 환경생물, 38(1), pp.106~113 (2020), 한국환경생물학회[DOI]
- 박인석, "Comparative analysis of sectioned-body morphometric characteristics of diploid and triploid marine medaka, Oryzias dancena", 환경생물, 38(1), pp.137~145 (2020), 한국환경생물학회[DOI]
- Park IS, "Karyotypes and cellular DNA contents of two species in the genus Lateolabrax from Korea", Fish. Sci., 65, pp.488~489 (1999)
- Thorgaard GH, "Triploid rainbow trout identified by flow cytometry", Aquaculture, 29, pp.305~309 (1982)
- Vanparys C, "Flow cytometric cell cycle analysis allows for rapid screening of estrogenicity in MCF-7breast cancer cells", Toxicol. Vitro, 20, pp.1238~1248 (2006)
- Wolters WR, "Erythrocyte nuclear measurements of diploid and triploid channel catfish, Ictalurus punctatus (Rafinicsque)", J. Fish Biol., 20, pp.253~258 (1982)