Chinese Hamster Lung Cell의 소핵시험을 이용한 식물추출물 유기농업자재의 유전독성평가
Genotoxicity of Environment-friendly Organic Materials of Plant Origin in the Micronucleus Test Using Chinese Hamster Lung Cells
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BACKGROUND: Azadirachta Indica extract(AIE) andSophorae radix extract(SRE) are widely used asenvironment-friendly organic materials of plant origin inSouth Korea. METHODS AND RESULTS: In this study, the in vitromicronucleus(vitMN) tests of two samples of AIE and SREwere conducted to evaluate their genotoxicity using theChinese hamster lung(CHL) cell. This study was composedof two parts; cytochalasin B(cyto B) test and non-cyto Btest. Mitomycin C and colchicine were used as positivecontrols. As a result, the incidence of micronucleus(MN) inall AIE and SRE treated groups increased in dosedependentmanner, but were less than 2.2% in 1,000binucleated cells. In addition, there were no significantincreases of MN incidence in all AIE and SRE treatedgroups, compared with the negative control group. CONCLUSION: Therefore, we suggest that AIE samplesand SRE samples used in this study may have nogenotoxicity in the in vitro micronucleus test using the CHLcells. In our previous study, we reported that AIE and SREdid not cause genotoxicity in Ames test. According to thegenotoxicity battery system, we concluded that AIE andSRE used in this study have no genotoxic effects to humans.
Abstract
BACKGROUND: Azadirachta Indica extract(AIE) andSophorae radix extract(SRE) are widely used asenvironment-friendly organic materials of plant origin inSouth Korea. METHODS AND RESULTS: In this study, the in vitromicronucleus(vitMN) tests of two samples of AIE and SREwere conducted to evaluate their genotoxicity using theChinese hamster lung(CHL) cell. This study was composedof two parts; cytochalasin B(cyto B) test and non-cyto Btest. Mitomycin C and colchicine were used as positivecontrols. As a result, the incidence of micronucleus(MN) inall AIE and SRE treated groups increased in dosedependentmanner, but were less than 2.2% in 1,000binucleated cells. In addition, there were no significantincreases of MN incidence in all AIE and SRE treatedgroups, compared with the negative control group. CONCLUSION: Therefore, we suggest that AIE samplesand SRE samples used in this study may have nogenotoxicity in the in vitro micronucleus test using the CHLcells. In our previous study, we reported that AIE and SREdid not cause genotoxicity in Ames test. According to thegenotoxicity battery system, we concluded that AIE andSRE used in this study have no genotoxic effects to humans.
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