The Effect of Light and CO2 on the Change of Electrical Potential Difference in Isolated Epidermis and Intact Leaves of Commeina communis L.
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Abstract
The effects of light and CO2 on the electrophysiological characteristics of guard cellsin the intact leaf and isolated epidermis have been investigated. Fast hyperpolarization of guardresponse to light and CO2. Whenever the experiments were attempted with isolated epidermis,there was no response to light and CO2. In order to determine the influence of the mesophyll cells,the apoplastic PD of guard cells in isolated epidermis was measured in the presence of themesophyll supernatant or the control medium. The apoplastic PD in isolated epidermis washyperpolarized to ≠7 mV, changing from ≠22 mV to ≠29 mV at 40 min. But, when isolatedepidermis was incubated with the supernatant from mesophyll cells incubated in the light, theapoplastic PD in isolated epidermis was hyperpolarized to ≠19 mV, changing from ≠22 mV to≠40.5mV. CO2 also caused a change of 0.1 to 0.3pH unit in the intact leaf. However, this changewas absent in isolated epidermis. A vibrating probe was used to detect the change in electricalintact leaves in the dark was 0.5A cm-2, remaining steady until illuminated. Light increased thecurrent on the surface of excised leaves to about 0.8A cm-2. However, light had no effect in thecurrent on the surface of isolated epidermis. Apoplastic pH changes across the stomatal complexin response to light and dark were measured both in the intact leaves and isolated epidermis over-electrodes. The guard cell wall of intact leaf was acidifiedto 2.5pH unit, falling from pH7.5 to pH5.0 in the first 10min. in the light. At the same time theguard cell wall pH of isolated epidermis fell from pH7.5 to pH7.0 at 10min. The guard cell wallpH of isolated epidermis incubated in the mesophyll supernatant fell from pH7.6 to pH6.7 at 10min. Likewise, It could be imagined that an electrical signal, chemicals and hormones propagatedfrom the mesophyll in response to light and CO2 could control a fast stomatal response.
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