Quantitative Proteomics towards Understanding Life and Environment
0
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
New proteomic techniques have been pioneered extensively in recent years, enabling the high- throughput and systematic analyses of cellular proteins in combination with bioinformatic tools. Furthermore, the development of such novel proteomic techniques facilitates the elucidation of the functions of proteins under stress or disease conditions, resulting in the discovery of biomarkers for responses to environmental stimuli. The ultimate objective of proteomics is targeted toward the entire proteome of life, subcellular localization, biochemical activities, and the regulation thereof. Comprehensive analysis strategies of proteomics can be classified into three categories: (i) protein separation via 2-dimensional gel electrophoresis (2-DE) or liquid chromatography (LC), (ii) protein identification via either Edman sequencing or mass spectrometry (MS), and (iii) proteome quantitation. Currently, MS-based proteomics techniques have shifted from qualitative proteome analysis via 2-DE or 2D-LC coupled with off-line matrix assisted laser desorption ionization (MALDI) and on-line electrospray ionization (ESI) MS, respectively, toward quantitative proteome analysis. In vitro quantitative proteomic techniques include differential gel electrophoresis with fluorescence dyes, protein-labeling tagging with isotope-coded affinity tags, and peptide-labeling tagging with isobaric tags for relative and absolute quantitation. In addition, stable isotope-labeled amino acids can be in vivo labeled into live culture cells via metabolic incorporation. MS-based proteomics techniques extend to the detection of the phosphopeptide mapping of biologically crucial proteins, which are associated with post-translational modification. These complementary proteomic techniques contribute to our current understanding of the manner in which life responds to differing environment.
참고문헌 (26)
- "Humphery-Smith,Fig. 15. SILAC in vivo labeling method scheme. An equivalent number of two sets of cells were grown on minimal media with either normal amino acid (generally used in 1H", 12C, 13C, pp.15N-labeled arginine). These mass tags have been subsequently combined and allowed for the quantitation of the relative abundance and identification of proteins via MS and MS, MS analysis, respectively. I., Hochstrasser, D. F., and Williams, K. L.
- "Exploring the metabolic and genetic control of gene expression in a genomic scale.", 278, pp.680~686 (1997)
- "Correlation between protein and mRNA abundance in yeast.", 19, pp.1720~1730 (1999)
- "Two-dimensional electrophoresis resources available from ExPASy.", 20, pp.3568~3571 (1999)
- "The sequence of the human genome.", 291, pp.1304~1351 (2001)
- "Genome sequence of the nematode Caenorhabditis elegans. A platform for investigating biology.", 282, pp.2012~2018 (1998)
- "A genomic regulatory network for development.", 295, pp.1669~1678 (2002)
- "Proteomics. Proteomics in genomeland.", 291, pp.1221~1224 (2001)
- "Shotgun identification of protein modifications from protein complexes and lens tissue.", 99, pp.7900~7905 (2002)
- "Analysis of aromatic catabolic pathways in Pseudomonas putida KT2440 using a combined proteomic approach", 6, pp.1301~318 (2006)
- "Approach to staionary two-dimensional pattern influence of focusing time and immobiline", carrier ampholytes concentrations. Electrophoresis 9, 1988, 37~46
- "Identification of novel MAP kinase pathway signaling targets by functional proteomics and mass spectrometry.", 6, pp.1343~1354 (2000)
- "Difference gel electrophoresis: a single gel method for detecting changes in protein extracts.", 18, pp.2071~2077 (1997)
- "Large-scale analysis of the yeast proteome by multidimensional protein identification technology.", 19, pp.242~247 (2001)
- "A method for the determination of the amino acid sequence in peptides. Arch Biochem 22" (1949)
- "A role of Edman degradation in proteome studies.", 18, pp.1068~1072 (1997)
- "Proteome analysis of light- induced proteins in Synechocystis sp. PCC 6803 Identification of proteins separated by 2D-PAGE using N-terminal sequencing and MALADI-OF MS.", 10, pp.705~711 (2000)
- "kinases and posphatases: the yin and yang of protein phosphorylation and signaling.", 80, pp.225~236 (1995)
- "Cellular signaling by receptor tyrosine kinases. Harvey Lect. 89" (1993)
- "a new approach to protein fractionation. Nature 258", pp.598~599 (1975)
- "Titania as a chemo-affinity support for the column-switching HPLC analysis of phosphopeptides application to the characterization of phosphorylation sites in proteins by combination with protease digestion and electrospray ionization mass spectrometry.", 20, pp.861~864 (2004)
- "Quantitative analysis of complex protein mixtures using isotope- coded affinity tags.", 17, pp.994~999 (1999)
- "Mass spectrometric analysis of protein mixtures at low levels using cleavable 13C-isotope- coded affinity tag and multidimensional chromatography.", 2, pp.299~314 (2003)
- "Multiplexed protein quantitation in Saccharomyces cervisiae using amine- reactive isobaric tagging reagents.", 3, pp.1154~1169 (2004)
- "Stable isotope labeling by amino acids in cell culture as a simple and accurate approach to expression proteomics.", 1, pp.376~386 (2002)
- "Global survey of organ and organelle protein expression in mouse: combined proteomic and transcriptomic profiling.", 125(i), pp.173~186 (2006)