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Lead Tolerance Profile of Pseudomonas Stuzeri in Liquid Culture

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Abstract

Pseudomonas stutzeri strain KCCM 34719 was used in this experiment to determine the effects of increasing Pb(II) concentrations on its growth rate. To obtain optimum growth conditions, strain KCCM 34719 was cultivated in nutrient broth under various conditions, such as temperature, pH, and NaCl concentration. Optimal conditions for cell growth were 30℃ of temperature, 8.0 of pH, and 3% of NaCl concentration, respectively. Growth response of bacterial cell to Pb(II) showed tolerance to concentrations ranging from 10 to 100 mg l-1 in liquid culture, following a growth pattern similar to the control. Growth rate was greatly inhibited at 200 mg l-1 of Pb(II).

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