하천수의 생태독성을 파악하기 위한 황산화미생물의 이용가능성 평가
Assessment of Biological Toxicity Monitoring in Water Using Sulfur Oxidizing Bacteria
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
BACKGROUND: Inappropriate discharge of wastewaters and industrial effluents are becoming detrimental to the aquatic environment. The presence of toxic substances on wastewaters can be detected by physicochemical and biological methods. However, physicochemical methods do not give any information about biological toxicity. Therefore, in this study we tried to detect the presence of toxic substance on waters using sulfur-oxidizing bacteria (SOB) as a bioassay. MATERIALS AND RESULTS: The SOB biosensor was first stabilized using synthetic stream water and operated in both continuous and semi-continuous mode. When the SOB biosensor was operated in continuous mode, the effluent electrical conductivity (EC) stabilized at∼1.72 dS/m. While in the case of semi-continuous, the EC stabilized at∼0.6 dS/m. The SOB system was also operated at different reaction times to ascertain the shortest reaction time for monitoring the toxicity. Finally, the SOB biosensor was fed with nitrite as toxic substance. When 5 mg/L of nitrite was added to the SOB system, the EC decreased immediately. However, the EC recovered after few cycle. CONCLUSION: This study shows that the SOB biosensor can be used as warning system to protect aquatic environment from hazardous materials. Although SOB biosensor can not give specific information about the toxic substances, it can assess whether the water is toxic or not.
Abstract
BACKGROUND: Inappropriate discharge of wastewaters and industrial effluents are becoming detrimental to the aquatic environment. The presence of toxic substances on wastewaters can be detected by physicochemical and biological methods. However, physicochemical methods do not give any information about biological toxicity. Therefore, in this study we tried to detect the presence of toxic substance on waters using sulfur-oxidizing bacteria (SOB) as a bioassay. MATERIALS AND RESULTS: The SOB biosensor was first stabilized using synthetic stream water and operated in both continuous and semi-continuous mode. When the SOB biosensor was operated in continuous mode, the effluent electrical conductivity (EC) stabilized at∼1.72 dS/m. While in the case of semi-continuous, the EC stabilized at∼0.6 dS/m. The SOB system was also operated at different reaction times to ascertain the shortest reaction time for monitoring the toxicity. Finally, the SOB biosensor was fed with nitrite as toxic substance. When 5 mg/L of nitrite was added to the SOB system, the EC decreased immediately. However, the EC recovered after few cycle. CONCLUSION: This study shows that the SOB biosensor can be used as warning system to protect aquatic environment from hazardous materials. Although SOB biosensor can not give specific information about the toxic substances, it can assess whether the water is toxic or not.
참고문헌 (14)
- 김병석, "표준생태독성시험법 개발을 위한 한국산 물벼룩의 최적 사육수 선발", 농약과학회지, 10(3), pp.189~195 (2006), 한국농약과학회
- Bozeman, J., "Toxicity testing using immobilized algae", Aquatic Toxicol, 14, pp.345~352 (1989)
- Gu, M.B., "A multi-channel continuous toxicity monitoring system using recombinant bioluminescent bacteria for classification of toxicity", Biosens. Bioelectron, 16, pp.661~666 (2001)
- Hernando, M.D., "Toxicity assays applied to wastewater treatment", Talanta, 65, pp.358~366 (2005)
- 김금용, "물벼룩을 이용한 중금속 급성 독성 실험", 한국수처리학회지, 18(3), pp.37~47 (2010), 한국수처리학회
- Lovley, D.R., "Novel mode of microbial energy metabolism: organic carbon oxidation coupled to dissimilatory reduction of iron or manganese", Appl. Environ. Microbiol, 54, pp.1472~1480 (1988)
- 오상은, "황산화미생물을 이용한 새로운 수(水)중 생태독성탐지 방법", 대한환경공학회지, 32(6), pp.555~562 (2010), 대한환경공학회
- Oh, S.E., "A novel biosensor for detecting toxicity in water using sulfur-oxidizing bacteria", Sensor Actuat B-Chemical, 154, pp.17~21 (2011)
- Sorokin, D.Y., "Haloalkaliphilic sulfur-oxidizing bacteria in soda lakes", FEMS Microbiol. Reviews, 29, pp.685~702 (2005)
- Strigui, N., "Tungsten speciation and toxicity: Acute toxicity of mono-and poly-tungstates to fish", Ecotox. Environ. Safe, 73, pp.164~171 (2010)
- Ginkel, S.W., "Detecting endocrine disrupting compounds in water using sulfur oxidizing bacteria", Chemosphere, 81, pp.294~297 (2010)
- Van Wezel, A., "New methods to monitor emerging chemicals in the drinking water production chain", J. Environ. Monitor, 12, pp.80~89 (2010)
- Woutersen, M., "Are luminescent bacteria suitable for online detection and monitoring of toxic compounds in drinking water and its sources", Anal. Bioanal. Chem, 400, pp.915~929 (2011)
- 윤성진, "국내산 물벼룩 Daphnia sp.를 이용한 연속적인 수질모니터링 장치 개발", 한국물환경학회지, 24(1), pp.36~43 (2008), 한국물환경학회