Morphology and molecular phylogeny of harmful dinoflagellate Pseudocochlodinium profundisulcus from Korean coastal area
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The morphological features of germling cells were examined to identify an unspecified resting cyst (described as Cochlodinium cf. polykrikoides-like resting cyst) in the Korean coastal area. LSU rRNA gene sequences were also obtained from a strain established from the germling cells. The resting cysts isolated from Korean coastal sediment were characterized as being brown in color, having a large dark-red body, and fibrous lobed ornaments. The germling cells were ellipsoidal with an irregular outline and had an open comma-shaped ASC (apical structure complex), a wide and deep cingulum, and a deep sulcus. These morphological features were consistent with those of previously described harmful dinoflagellate Pseudocochlodinium profundisulcus. The molecular phylogeny revealed that the germling cells and P. profundisulcus were conspecific. Based on these morphological and phylogenetic data, this study documents the occurrence of P. profundisulcus in a Korean coastal area for the first time.
Abstract
The morphological features of germling cells were examined to identify an unspecified resting cyst (described as Cochlodinium cf. polykrikoides-like resting cyst) in the Korean coastal area. LSU rRNA gene sequences were also obtained from a strain established from the germling cells. The resting cysts isolated from Korean coastal sediment were characterized as being brown in color, having a large dark-red body, and fibrous lobed ornaments. The germling cells were ellipsoidal with an irregular outline and had an open comma-shaped ASC (apical structure complex), a wide and deep cingulum, and a deep sulcus. These morphological features were consistent with those of previously described harmful dinoflagellate Pseudocochlodinium profundisulcus. The molecular phylogeny revealed that the germling cells and P. profundisulcus were conspecific. Based on these morphological and phylogenetic data, this study documents the occurrence of P. profundisulcus in a Korean coastal area for the first time.
참고문헌 (20)
- Donald M. Anderson, "The globally distributed genus Alexandrium: Multifaceted roles in marine ecosystems and impacts on human health", Harmful Algae, 14, pp.10~35 (2012), Elsevier BV[DOI]
- Stéphane Guindon, "New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0", Systematic Biology, 59(3), pp.307~321 (2010), Oxford University Press (OUP)[DOI]
- Zhangxi Hu, "Morpho-molecular description of a new HAB species, Pseudocochlodinium profundisulcus gen. et sp. nov., and its LSU rRNA gene based genetic diversity and geographical distribution", Harmful Algae, 108, pp.102098~ (2021), Elsevier BV[DOI]
- Zhun LI, "Identification of the resting cyst of <i><scp>C<", scp>ochlodinium polykrikoides <, scp>argalef (<scp>D<, pp.scp>inophyceae, <scp>G<, scp>ymnodiniales) in <scp>K<[DOI]
- Matsuoka K, "Technical Guide for Modern Dinoflagellate Cyst Study. WESTPAC-HAB", WESTPAC, Asian Natural Environmental Science Center, pp.2000, ~
- Tatiana Yu. Orlova, "Dinoflagellate cysts in recent marine sediments from the east coast of Russia", Botanica Marina, 47(3), pp.184~201 (2004), Walter de Gruyter GmbH[DOI]
- Park JS, "An environmental analysis of distribution of dinoflagellate cysts in the southwest sea of Korea and a taxonomic study through germination", Ph. D., pp.2007
- Qi Y, "Taxonomy differentiation of harmful algal bloom causative species in genus Cochlodinium in Chinese coastal waters", Proceedings of the Fifteen Academic Conference and Celebration of 30-year Anniversary for Chinese Society of Phycology, pp.15~18 (2009)
- Dajun Qiu, "Apical Groove Type and Molecular Phylogeny Suggests Reclassification of Cochlodinium geminatum as Polykrikos geminatum", PLoS ONE, 8(8), pp.e71346~ (2013), Public Library of Science (PLoS)[DOI]
- Fredrik Ronquist, "MrBayes 3: Bayesian phylogenetic inference under mixed models", Bioinformatics, 19(12), pp.1572~1574 (2003), Oxford University Press (OUP)[DOI]
- Lixia Shang, "Pseudocochlodinium profundisulcus Resting Cysts Detected in the Ballast Tank Sediment of Ships Arriving in the Ports of China and North America and the Implications in the Species’ Geographic Distribution and Possible Invasion", International Journal of Environmental Research and Public Health, 19(1), pp.299~ (2021), MDPI AG[DOI]
- Hyeon Ho Shin, "Dinoflagellate Cysts in Surface Sediments from Southern Coast of Korea", Estuaries and Coasts, 34(4), pp.712~725 (2011), Springer Science and Business Media LLC[DOI]
- Hyeon Ho Shin, "Centrodinium punctatum (Dinophyceae) produces significant levels of saxitoxin and related analogs", Harmful Algae, 100, pp.101923~ (2020), Elsevier BV[DOI]
- Hyeon Ho Shin, "Molecular phylogeny and morphology of <i>Torquentidium<", i> gen. et comb. nov. for <i>Cochlodinium convolutum<, European Journal of Phycology, pp.2019, 54(3)[DOI]
- 신현호, "Harmful Dinoflagellate Prorocentrum donghaiense Lu is Widely Distributed Along the East China Sea and Korean Coastal Area", Ocean Science Journal, 54(4), pp.685~691 (2019), 한국해양과학기술원[DOI]
- Yoshihito Takano, "ACQUIRING SCANNING ELECTRON MICROSCOPICAL, LIGHT MICROSCOPICAL AND MULTIPLE GENE SEQUENCE DATA FROM A SINGLE DINOFLAGELLATE CELL<sup>1<", sup>, 2006, pp.42(1), 251~256[DOI]
- Ying Zhong Tang, "The toxic dinoflagellate Cochlodinium polykrikoides (Dinophyceae) produces resting cysts", Harmful Algae, 20, pp.71~80 (2012), Elsevier BV[DOI]
- Gaurav Vaidya, "SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information", Cladistics, 27(2), pp.171~180 (2011), Wiley[DOI]
- Wang Z, "Effects of Cochlodinium bloom in Pearl River Estuary in China on the growth of other harmful algal bloom species", J. Shenzhen Univ. Sci. Eng, 28, pp.553~558 (2011)
- Aika Yamaguchi, "Molecular phylogenetic study of the heterotrophic dinoflagellate genus Protoperidinium (Dinophyceae) inferred from small subunit rRNA gene sequences", Phycological Research, 53(1), pp.30~42 (2005), Wiley[DOI]