Yeasts Associated with Roots of the Endemic Plant Mankyua chejuense
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BACKGROUND: Identification of endophytic yeasts inhabiting the internal roots of the Mankyua chejuense tree requires techniques involving biotechnology. There is a need for a culture-based method to isolate and identify yeast strains associated with M. chejuense. METHODS AND RESULTS: We spread homogenized M. chejuense root samples onto glucose-peptone- yeast agar containing antibiotics, Triton X-100, and L-sorbose. A total of 152 yeast isolates were obtained and identified via phylogenetic analysis based on ITS gene sequencing. The results revealed that the root-associated yeast species included the genera Cyberlindnera (140 isolates), Candida (11 isolates), and Kluyveromyces (one isolate). Additionally, three yeast isolates showed high bioethanol production. CONCLUSION: We identified the specific yeast community associated with M. chejuense roots. These yeast isolates may have industrial applications as bioethanol producers. Our findings revealed that Cyberlindnera isolates included C. suaverolens and C. satumus, while Kluyveromyces isolates showed high bioethanol production.
Abstract
BACKGROUND: Identification of endophytic yeasts inhabiting the internal roots of the Mankyua chejuense tree requires techniques involving biotechnology. There is a need for a culture-based method to isolate and identify yeast strains associated with M. chejuense. METHODS AND RESULTS: We spread homogenized M. chejuense root samples onto glucose-peptone- yeast agar containing antibiotics, Triton X-100, and L-sorbose. A total of 152 yeast isolates were obtained and identified via phylogenetic analysis based on ITS gene sequencing. The results revealed that the root-associated yeast species included the genera Cyberlindnera (140 isolates), Candida (11 isolates), and Kluyveromyces (one isolate). Additionally, three yeast isolates showed high bioethanol production. CONCLUSION: We identified the specific yeast community associated with M. chejuense roots. These yeast isolates may have industrial applications as bioethanol producers. Our findings revealed that Cyberlindnera isolates included C. suaverolens and C. satumus, while Kluyveromyces isolates showed high bioethanol production.
참고문헌 (20)
- Botha, A., "The importance and ecology of yeasts in soil", Soil Biology and Biochemistry, 43(1), pp.1~8 (2011)
- 최성창, "알로에 베라(A. vera)와 알로에 사포나리아(A. saponaria)로 부터 효모의 분리 및 계통분석", 한국환경농학회지, 32(3), pp.240~243 (2013), 한국환경농학회[DOI]
- Deak, T., "Ecology and biodiversity of yeasts with potential value in biotechnology. Yeast biotechnology:diversity and applications", pp.151~168 (2009), Springer Science + Business Media B. V.
- Fonseca, A., "Phylloplane yeasts. Biodiversity and ecophysiology of yeasts", pp.263~301 (2006), Springer
- 현화자, "제주고사리삼(Mankyua chejuense B.-Y. Sun, M.H. Kim & C.H. Kim)의 생장 형태 및 식물계절학적 특성", 한국자원식물학회지, 27(1), pp.95~101 (2014), 한국자원식물학회[DOI]
- 현화자, "제주고사리삼 자생지의 환경 및 식물상", 한국자원식물학회지, 23(4), pp.350~359 (2010), 한국자원식물학회
- Kim, C. H., "Conservation status of the endemic fern Mankyua chejuense (Ophioglossaceae) on Cheju Island, Republic of Korea", Oryx, 38(2), pp.217~219 (2004)
- 김종식, "야생 참나리(Lilium lancifolium Thunb.)로부터 분리한 효모의 분자계통학적 분석", 한국환경농학회지, 34(2), pp.149~154 (2015), 한국환경농학회[DOI]
- "한국환경농학회지", 2015, 238~243, pp.한국환경농학회, http:
- Kim, J. S., "A novel biosurfactant production by Aureobasidium pullulans L3-GPY from a Tiger lily wild flower, Lilium lancifolium Thunb", PLoS One, 10(4), pp.e0122917~ (2015)
- Jong-Shik Kim, "Aureosurfactin and 3-deoxyaureosurfactin, novel biosurfactants produced by Aureobasidium pullulans L3-GPY", The Journal of Antibiotics, 69(10), pp.759~761 (2016), Springer Nature[DOI]
- 이경미, "멸종위기 식물 제주고사리삼의 입지와 식생구조의 특징에 대한 연구", 한국습지학회지, 14(1), pp.35~45 (2012), 한국습지학회
- Poomtien, J., "Production and characterization of a biosurfactant from Cyberlindnera samutprakarnensis JP52T", Bioscience, Biotechnology, and Biochemistry, 77(12), pp.2362~2370 (2013)
- Raspor, P., "Yeast in extreme environments. Biodiversity and ecophysiology of yeasts", pp.370~417 (2006), Springer
- Rosen, M. J., "Surfactants and Interfacial Phenomena", pp.471~501 (2012), John Wiley &Sons
- Saitou, N., "The neighbor-joining method: a new method for reconstructing phylogenetic trees", Molecular Biology and Evolution, 4(4), pp.406~425 (1987)
- Sun, B. Y., "Mankyua (Ophioglossaceae): A new fern genus from Cheju Island, Korea", Taxon, 50(4), pp.1019~1024 (2001)
- Tamang, J. P., "Yeasts diversity in fermented foods and beverages. Yeast biotechnology:diversity and applications", pp.169~198 (2009), Springer Science + Business Media B.V.
- Tamura, K., "MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods", Molecular Biology and Evolution, 28(10), pp.2731~2739 (2011)
- White, T. J., "Amplication and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications", pp.315~322 (1990), Academic press