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토마토에 염류 내성을 유도하는 바실러스 균주 처리 후 근권 미생물 군집 구조 연구

Assessment of Rhizosphere Microbial Community Structure in Tomato Plants after Inoculation of Bacillus Species for Inducing Tolerance to Salinity

이신애|원항연|송재경|상미경|유성제
한국환경농학회 (The Korean Society Of Environmental Agriculture)|2021.03|한국환경농학회지 (Agricultural and Environmental Sciences)|제40권 제1호|pp. 49-59|DOI https://doi.org/10.5338/KJEA.2021.40.1.6

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참고문헌

키워드

SalinityTomatoBacillusMicrobial Diversity
주제분류농학

초록

BACKGROUND: Soil salinity causes reduction of crop productivity. Rhizosphere microbes have metabolic capa-bilities and ability to adaptation of plants to biotic and abiotic stresses. Plant growth-promoting bacteria (PGPB) could play a role as elicitors for inducing tolerance to stresses in plants by affecting resident microorganism in soil. This study was conducted to demonstrate the effect of selected strains on rhizosphere microbial community under salinity stress. METHODS AND RESULTS: The experiments were con-ducted in tomato plants in pots containing field soil. Bacterial suspension was inoculated into three-week-old tomato plants, one week after inoculation, and -1,000 kPa-balanced salinity stress was imposed. The physiological and bio-chemical attributes of plant under salt stress were monitored by evaluating pigment, malondialdehyde (MDA), proline, soil pH, electrical conductivity (EC) and ion concentrations. To demonstrate the effect of selected Bacillus strains on rhi-zosphere microbial community, soil microbial diversity and abundance were evaluated with Illumina MiSeq sequencing, and primer sets of 341F/805R and ITS3/ITS4 were used for bacterial and fungal communities, respectively. As a result, when the bacterial strains were inoculated and then salinity stress was imposed, the inoculation decreases the stress sus-ceptibility including reduction in lipid peroxidation, en-hanced pigmentation and proline accumulation which sub-sequently resulted in better plant growth. However, bacterial inoculations did not affect diversity (observed OTUs, ACE, Chao1 and Shannon) and structure (principle coordinate analysis) of microbial communities under salinity stress. Furthermore, relative abundance in microbial communities had no significant difference between bacterial treated- and untreated-soils under salinity stress. CONCLUSION: Inoculation of Bacillus strains could affect plant responses and soil pH of tomato plants under salinity stress, whereas microbial diversity and abundance had no significant difference by the bacterial treatments. These findings demonstrated that Bacillus strains could alleviate plant’s salinity damages by regulating pigments, proline, and MDA contents without significant changes of microbial community in tomato plants, and can be used as effective biostimulators against salinity stress for sustainable agriculture.

Abstract

BACKGROUND: Soil salinity causes reduction of crop productivity. Rhizosphere microbes have metabolic capa-bilities and ability to adaptation of plants to biotic and abiotic stresses. Plant growth-promoting bacteria (PGPB) could play a role as elicitors for inducing tolerance to stresses in plants by affecting resident microorganism in soil. This study was conducted to demonstrate the effect of selected strains on rhizosphere microbial community under salinity stress. METHODS AND RESULTS: The experiments were con-ducted in tomato plants in pots containing field soil. Bacterial suspension was inoculated into three-week-old tomato plants, one week after inoculation, and -1,000 kPa-balanced salinity stress was imposed. The physiological and bio-chemical attributes of plant under salt stress were monitored by evaluating pigment, malondialdehyde (MDA), proline, soil pH, electrical conductivity (EC) and ion concentrations. To demonstrate the effect of selected Bacillus strains on rhi-zosphere microbial community, soil microbial diversity and abundance were evaluated with Illumina MiSeq sequencing, and primer sets of 341F/805R and ITS3/ITS4 were used for bacterial and fungal communities, respectively. As a result, when the bacterial strains were inoculated and then salinity stress was imposed, the inoculation decreases the stress sus-ceptibility including reduction in lipid peroxidation, en-hanced pigmentation and proline accumulation which sub-sequently resulted in better plant growth. However, bacterial inoculations did not affect diversity (observed OTUs, ACE, Chao1 and Shannon) and structure (principle coordinate analysis) of microbial communities under salinity stress. Furthermore, relative abundance in microbial communities had no significant difference between bacterial treated- and untreated-soils under salinity stress. CONCLUSION: Inoculation of Bacillus strains could affect plant responses and soil pH of tomato plants under salinity stress, whereas microbial diversity and abundance had no significant difference by the bacterial treatments. These findings demonstrated that Bacillus strains could alleviate plant’s salinity damages by regulating pigments, proline, and MDA contents without significant changes of microbial community in tomato plants, and can be used as effective biostimulators against salinity stress for sustainable agriculture.

참고문헌 (50)

  1. Willcox JK, "Tomatoes and cardiovascular health", Critical Reviews in Food Science and Nutrition, 43, pp.1~18 (2003)[DOI]
  2. Gerszberg A, "Tomato (Solanum lycopersicum L.) in the service of biotechnology", Plant Cell, Tissue and Organ Culture (PCTOC), 120, pp.881~902 (2015)[DOI]
  3. Ayyogari K, "Impact of climate change on vegetable cultivation-a review", International Journal of Agriculture, Environment and Biotechnology, 7(1), pp.145~155 (2014)[DOI]
  4. Shamshiri RR, "Review of optimum temperature, humidity, and vapour pressure deficit for microclimate evaluation and control in greenhouse cultivation of tomato : A review", International Agrophysics, 32, pp.287~302 (2018)[DOI]
  5. Putranta H, "The effect of pH, electrical conductivity, and nitrogen(N)in the soil at Yogyakarta special region on tomato plant growth", TEM Journal, 8(3), pp.860~865 (2019)
  6. Shrivastava P, "Soil salinity : A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation", Saudi Journal of Biological Sciences, 22(2), pp.123~131 (2015)[DOI]
  7. Bharti N, "Halotolerant PGPRs prevent major shifts in indigenous microbial community structure under salinity stress", Microbial Ecology, 70(1), pp.196~208 (2015)[DOI]
  8. Moreno-Limon S, "Genotypic variability in bean cultivars(Phaseolus vulgaris L. )for resistance to salinity at the seedling stage", Indian Agriculturist, 44, pp.1~12 (2000)
  9. Zongsuo L, "Study on types of water stress adaptation in both Brassica napus L. and Brassica juncea L", Acta Botanica Borealioccidentalia Sinica, 01 (1992)
  10. Li HW, "Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice", Planta, 234(5), pp.1007~1018 (2011)[DOI]
  11. Muñoz-Mayor A, "Overexpression of dehydrin tas14 gene improves the osmotic stress imposed by drought and salinity in tomato", Journal of Plant Physiology, 169, pp.459~468 (2012)[DOI]
  12. Chen L, "Induced maize salt tolerance by rhizosphere inoculation of Bacillus amyloliquefaciens SQR9", Physiologia Plantarum, 158(1), pp.34~44 (2016)[DOI]
  13. Bhattacharyya PN, "Plant growth-promoting rhizobacteria(PGPR) : Emergence in agriculture", World Journal of Microbiology and Biotechnology, 28(4), pp.1327~1350 (2012)[DOI]
  14. El-Esawi MA, "Serratia liquefaciens KM4 improves salt stress tolerance in maize by regulating redox potential, ion homeostasis, leaf gas exchange and stress-related gene expression", International Journal of Molecular Science, 19, pp.3310~ (2018)[DOI]
  15. Silini A, "Growing varieties durum wheat(Triticum durum)in response to the effect of osmolytes and inoculation by Azotobacter chroococcum under salt stress", African Journal of Microbiology Research, 10(12), pp.387~399 (2016)[DOI]
  16. Akram MS, "Deciphering Staphylococcus sciuri SAT-17 mediated anti-oxidative defense mechanisms and growth modulations in salt stressed maize(Zea mays L. )", Frontiers in Microbiology, 7, pp.867~ (2016)[DOI]
  17. Mendes R, "The rhizosphere microbiome : significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms", FEMS Microbiology Reviews, 37(5), pp.634~663 (2013)[DOI]
  18. Fu L, "Inducing the rhizosphere microbiome by biofertilizer application to suppress banana fusarium wilt disease", Soil Biology and Biochemistry, 104, pp.39~48 (2017)[DOI]
  19. Xue L, "Soil bacterial community structure and co-occurrence pattern during vegetation restoration in karst rocky desertification area", Frontiers in Microbiology, 8, pp.2377~ (2017)[DOI]
  20. Bharti N, "Co-inoculation of Dietzia natronolimnaea and Glomus intraradices with vermicompost positively influences Ocimum basilicum growth and resident microbial community structure in salt affected low fertility soils", Applied Soil Ecology, 100, pp.211~225 (2016)[DOI]
  21. 유성제, "Induced Tolerance to Salinity Stress by Halotolerant Bacteria Bacillus aryabhattai H19-1 and B. mesonae H20-5 in Tomato Plants", Journal of Microbiology and Biotechnology, 29(7), pp.1124~1136 (2019), 한국미생물·생명공학회[DOI]
  22. Vacheron J, "Fluorescent Pseudomonas strains with only few plant-beneficial properties are favored in the maize rhizosphere", Frontiers in Plant Science, 7, pp.1212~ (2016)[DOI]
  23. Piromyou P, "Effect of plant growth promoting rhizobacteria(PGPR)inoculation on microbial community structure in rhizosphere of forage corn cultivated in Thailand", European Journal of Soil Biology, 47(1), pp.44~54 (2011)[DOI]
  24. Chihaoui SA, "Inoculation of Phaseolus vulgaris with the nodule-endophyte Agrobacterium sp. 10C2affects richness and structure of rhizosphere bacterial communities and enhances nodulation and growth", Archives of Microbiology, 197, pp.805~813 (2015)[DOI]
  25. 유성제, "토마토 염류와 온도 스트레스에 대한내성을 유도하는 미생물 선발", 한국유기농업학회지, 26(3), pp.463~475 (2018), 한국유기농업학회
  26. Polonenko DR, "Microbial responses to salt-induced osmotic stress", Plant and Soil, 59, pp.269~285 (1981)
  27. Fernandez O, "Burkholderia phytofirmans PsJN acclimates grapevine to cold by modulating carbohydrate metabolism", Molecular Plant-Microbe Interactions, 25, pp.496~504 (2012)[DOI]
  28. Lichtenthaler HK, "Chlorophyll fluorescence signatures of leaves during the autumnal chlorophyll breakdown", Journal of Plant Physiology, 131, pp.101~110 (1987)[DOI]
  29. Bates LS, "Rapid determination of free proline for water-stress studies", Plant and Soil, 39, pp.205~207 (1973)[DOI]
  30. Tiwari S, "Bacillus amyloliquefaciens confers tolerance to various abiotic stresses and modulates plant response to phytohormones through osmoprotection and gene expression regulation in rice", Frontiers in Plant Science, 8, pp.1510~ (2017)[DOI]
  31. Liu RX, "Effects of N fertilization on root development and activity of water-stressed cotton(Gossypium hirsutum L. )plants", Agricultural Water Management, 95, pp.1261~1270 (2008)
  32. 조현숙, "The Changes of Soil Carbon as Affected by Several Kinds of Organic Material in Upland Soil", 한국토양비료학회지(Korean Journal of Soil Science and Fertilizer), 51(4), pp.586~595 (2018), 한국토양비료학회[DOI]
  33. Qiao Q, "The variation in the rhizosphere microbiome of cotton with soil type, genotype and developmental stage", Scientific Reports, 7, pp.3940~ (2017)[DOI]
  34. Schloss PD, "Introducing mothur : open-source, platform-independent, community supported software for describing and comparing microbial communities", Applied and Environmental Microbiology, 75, pp.7537~7541 (2009)
  35. Badri DV, "Application of natural blends of phytochemicals derived from the root exudates of Arabidopsis to the soil reveal that phenolic-related compounds predominantly modulate the soil microbiome", Journal of Biological Chemistry, 288, pp.4502~4512 (2013)[DOI]
  36. Kong XQ, "Exogenous nitric oxide delays salt-induced leaf senescence in cotton(Gossypium hirsutum L. )", Acta Physiologiae Plantarum, 38, pp.61~ (2016)
  37. Turan S, "Salt-stress induced modulation of chlorophyll biosynthesis during de-etiolation of rice seedlings", Physiologia Plantarum, 153, pp.477~491 (2015)[DOI]
  38. Ruiz-Sola MÁ, "A root specific induction of carotenoid biosynthesis contributes to ABA production upon salt stress in Arabidopsis", PLoS ONE, 9, pp.e90765~ (2014)[DOI]
  39. Jamil M, "Salinity reduced growth PS2 photochemistry and chlorophyll content in radish", Scientia Agricola, 64, pp.111~118 (2007)[DOI]
  40. Hayat S, "Role of proline under changing environments : A review", Plant Signaling and Behavior, 7, pp.1456~1466 (2012)[DOI]
  41. Ben Rejeb K, "How reactive oxygen species and proline face stress together", Plant Physiology and Biochemistry, 80, pp.278~284 (2014)[DOI]
  42. Sevengor S, "The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling", African Journal of Agricultural Research, 6, pp.4920~4924 (2011)
  43. Tsikas D, "Assessment of lipid peroxidation by measuring malondialdehyde(MDA)and relatives in biological samples : Analytical and biological challenges", Analytical Biochemistry, 524, pp.13~30 (2017)[DOI]
  44. Soti PG, "Effect of soil pH on growth, nutrient uptake, and mycorrhizal colonization in exotic invasive Lygodium microphyllum", Plant Ecology, 216, pp.989~998 (2015)[DOI]
  45. De Boer W, "Nitrification in acid soils : Micro-organisms and mechanisms", Soil Biology and Biochemistry, 33, pp.853~866 (2001)[DOI]
  46. Nicol GW, "The influence of soil pH on the diversity, abundance and transcriptional activity of ammonia oxidizing archaea and bacteria", Environmental Microbiology, 10, pp.2966~2978 (2008)[DOI]
  47. Nautiyal CS, "Plant growth-promoting bacteria Bacillus amyloliquefaciens NBRISN13 modulates gene expression profile of leaf and rhizosphere community in rice during salt stress", Plant Physiology and Biochemistry, 66, pp.1~9 (2013)[DOI]
  48. Dawson W, "A small number of low-abundance bacteria dominate plant species-specific responses during rhizosphere colonization", Frontiers in Microbiology, 8, pp.975~ (2017)[DOI]
  49. Jousset A, "Where less may be more : how the rare biosphere pulls ecosystems strings", The ISME Journal, 11, pp.853~862 (2017)[DOI]
  50. Yurkov A, "Multigene assessment of the species boundaries and sexual status of the basidiomycetous yeasts Cryptococcus flavescens and C. Terrestris(tremellales)", PLoS One, 10, pp.e0120400~ (2015)[DOI]

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