아세트산 칼륨을 활용한 한국 논 토양에서 메탄 생성 미생물 군집 예측
Prediction of Methanogenic Microbial Community in Korean Rice Paddy Soil Using Potassium Acetate
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Methane emissions in paddy soils occur through the decomposition of organic matter by anaerobic bacteria, including methanogenic bacteria, under anaerobic conditions maintained by waterlogged soil environments. These methanogenic bacteria produce methane via the acetoclastic pathway or the hydrogenotrophic pathway, making it challenging to determine the primary pathway for methane production. In this study, we supplied potassium acetate (KOAc) instead of additional hydrogen supplements to limit methane production through the hydrogenotrophic pathway, thereby analyzing the conversion of acetate to methane in paddy soils. We investigated the effect of acetate ions on methane production at various concentrations and confirmed methane production upon acetate addition. Furthermore, genetic cluster analysis revealed that Archaea clusters likely respond to acetate ions to produce methane. Soil reaction analysis based on regional soil characteristics revealed significant variations in methane production in response to acetate ions, indicating that methane production pathways in paddy soils may vary regionally.
Abstract
Methane emissions in paddy soils occur through the decomposition of organic matter by anaerobic bacteria, including methanogenic bacteria, under anaerobic conditions maintained by waterlogged soil environments. These methanogenic bacteria produce methane via the acetoclastic pathway or the hydrogenotrophic pathway, making it challenging to determine the primary pathway for methane production. In this study, we supplied potassium acetate (KOAc) instead of additional hydrogen supplements to limit methane production through the hydrogenotrophic pathway, thereby analyzing the conversion of acetate to methane in paddy soils. We investigated the effect of acetate ions on methane production at various concentrations and confirmed methane production upon acetate addition. Furthermore, genetic cluster analysis revealed that Archaea clusters likely respond to acetate ions to produce methane. Soil reaction analysis based on regional soil characteristics revealed significant variations in methane production in response to acetate ions, indicating that methane production pathways in paddy soils may vary regionally.
참고문헌 (25)
- Saunois M, "The global methane budget : 2000–2012", Earth System Science Data Discussions, 2016, pp.1~79 (2016)[DOI]
- Wang J, "The role of rice cultivation in changes in atmospheric methane concentration and the Global Methane Pledge", Global Change Biology, 29(10), pp.2776~2789 (2023)[DOI]
- Jackson RB, "Increasing anthropogenic methane emissions arise equally from agricultural and fossil fuel sources", Environmental Research Letters, 15(7), pp.071002~ (2020)[DOI]
- Smith P, "Agricultural methane emissions and the potential formitigation", Philosophical Transactions of the Royal Society A, 379(2210), pp.20200451~ (2021)[DOI]
- Gwon HS, "Research review of methane emissions from Korean rice paddies", Journal of Climate Change Research, 13(1), pp.117~134 (2022)[DOI]
- Qian H, "Greenhouse gas emissions and mitigation in rice agriculture", Nature Reviews Earth & Environment, 4(10), pp.716~732 (2023)[DOI]
- Nan Q, "Exploring long-term effects of biochar on mitigating methane emissions from paddy soil : A review", Biochar, 3(2), pp.125~134 (2021)[DOI]
- de Bont JAM, "Bacterial oxidation of methane in a rice paddy", Ecological Bulletins, 26, pp.91~96 (1978)
- Horz HP, "Detection of methanotroph diversity on roots of submerged rice plants by molecular retrieval of pmoA, mmoX, mxaF, and 16S rRNA and ribosomal DNA, including pmoA-based terminal restriction fragment length polymorphism profiling", Applied and Environmental Microbiology, 67(9), pp.4177~4185 (2001)[DOI]
- Singh R, "Integrated system of anaerobic digestion and pyrolysis for valorization of agricultural and food waste towards circular bioeconomy", Bioresource Technology, 360, pp.127596~ (2022)[DOI]
- Yang Z, "Long-term evaluation of bioaugmentation to alleviate ammonia inhibition during anaerobic digestion : process monitoring, microbial community response, and methanogenic pathway modeling", Chemical Engineering Journal, 399, pp.125765~ (2020)[DOI]
- Wang HZ, "Identification of novel potential acetate-oxidizing bacteria in an acetate-fed methanogenic chemostat based on DNA stable isotope probing", The Journal of General and Applied Microbiology, 64(5), pp.221~231 (2018)[DOI]
- Park SG, "Methanogenesis stimulation and inhibition for the production of different target electrobiofuels in microbial electrolysis cells through an on-demand control strategy using the coenzyme M and 2-bromoethanesulfonate", Environment International, 131, pp.105006~ (2019)[DOI]
- Guo J, "Transcriptome analysis reveals that distinct metabolic pathways operate in salt-tolerant and salt-sensitive upland cotton varieties subjected to salinity stress", Plant Science, 238, pp.33~45 (2015)[DOI]
- Dyksma S, "Syntrophic acetate oxidation replaces acetoclastic methanogenesis during thermophilic digestion of biowaste", Microbiome, 8(1), pp.105~ (2020)[DOI]
- Fotidis IA, "Effect of ammonium and acetate on methanogenic pathway and methanogenic community composition", FEMS Microbiology Ecology, 83(1), pp.38~48 (2013)[DOI]
- Gwon HS, "Research review of methane emissions from Korean rice paddies", Journal of Climate Change Research, 13(1), pp.117~134 (2022)[DOI]
- Mun SW, "A study on the making properties of natural pigments based on substance characteristics for hwangto in Korea", Journal of Conservation Science, 35(6), pp.600~611 (2019)[DOI]
- Yang SS, "Effect of environmental conditions on methane production and emission from paddy soil", Agriculture, Ecosystems & Environment, 69(1), pp.69~80 (1998)[DOI]
- Qin Y, "A new cell-direct quantitative PCR based method to monitor viable genetically modified Escherichia coli", Korean Journal of Agricultural Science, 49(4), pp.795~807 (2022)[DOI]
- Sørensen KB, "Thermodynamic and kinetic requirements in anaerobic methane oxidizing consortia exclude hydrogen, acetate, and methanol as possible electron shuttles", Microbial Ecology, 42, pp.1~10 (2001)[DOI]
- Nozoe T, "Mechanisms of decomposition of acetic acid by methane-producing bacteria in submerged paddy soil", Soil Science and Plant Nutrition, 41(1), pp.151~155 (1995)[DOI]
- Amin FR, "Functions of bacteria and archaea participating in the bioconversion of organic waste for methane production", Science of the Total Environment, 763, pp.143007~ (2021)[DOI]
- Ji Y, "Response of fermenting bacterial and methanogenic archaeal communities in paddy soil to progressing rice straw degradation", Soil Biology and Biochemistry, 124, pp.70~80 (2018)[DOI]
- Wu L, "Composition of archaeal community in a paddy field as affected by rice cultivar and N fertilizer", Microbial Ecology, 58, pp.819~826 (2009)[DOI]
