미생물 접종 바이오차 펠렛을 이용한 배추 뿌리혹병 방제 및 온실가스 저감 효과

Abstract

This study aimed to evaluate the control efficacy of clubroot disease in Kimchi cabbage and carbon sequestration by applying microbial biochar pellets inoculated with antagonistic microorganisms. The microbial biochar pellets were prepared by mixing rice husk biochar and guano at a 6:4 ratio, followed by inoculation with collected antagonistic strains against clubroot disease, adjusting to a viable cell density above 1.0 × 107 CFU･mL-1 and a total nitrogen content of 6%. Treatments included a non-treated control, biochar alone, and biochar pellets inoculated with Pseudomonas fluorescens (RBPM27) or Bacillus megaterium (RBPM86). Before transplanting Kimchi cabbage in the spring trial field, each material was evenly mixed into the soil, and greenhouse gas samplings such as CH4 and N2O were analyzed at 7 day intervals during the growing period. Clubroot incidence was evaluated 50 days after transplanting. The RBPM86 treatment significantly reduced the incidence rate of clubroot by 31.8%, showing the highest control efficacy of 57.7%. The RBPM27 and RBPM86 treatment significantly reduced the cumulative emissions of CH4 and N2O by 69% and 29%, respectively, relative to the control, indicating its potential for greenhouse gas mitigation. These results suggest that microbial biochar pellets inoculated with effective antagonistic microorganisms offer a promising strategy for managing clubroot disease and mitigating greenhouse gas emissions in Kimchi cabbage cultivation.