다공성 규조토 컬럼을 이용한 지방함량이 다른 농산물 시료 중 잔류농약 분석법 개선

Abstract

Due to their similar properties to pesticides, lipids in agricultural products can interfere with the analysis of pesticide residues. This study tested the analytical recoveries of three pesticides with varying polarities, namely, bifenthrin, chlorpyrifos, and acetamiprid, across three agricultural products with different lipid contents: oriental melon, brown rice, and peanuts. The analysis was conducted using a method established by the Ministry of Food and Drug Safety. The results indicated that higher lipid content in agricultural products and lower polarity of pesticides were associated with lower recovery rates, leading to significant emulsion during the liquid-liquid partitioning process. To resolve the recovery issue, the liquid-liquid partition was replaced with acetonitrile as an extraction solvent and a macroporous diatomaceous earth column. The recovery rates using these improved analytical methods ranged from 81.8% to 94.8%, with coefficients of variation for triplicates between 0.7% and 5.6%. These results meet the criteria for pesticide residue analysis, which require recoveries of 70-120% and CVs of <10%. The methods developed in this study effectively enhance pesticide extraction from agricultural products while minimizing the effects of lipids and emulsion.