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익사에서 규조류 검사 방법과 신뢰도의 진전

Progress in Method and Reliability of Diatom Test in Drowning

허기영
대한법의학회 (The Korean Society For Legal Medicine)|2020.08|대한법의학회지 (The Korean Journal of Legal Medicine)|제44권 제3호|pp. 115-122|DOI https://doi.org/10.7580/kjlm.2020.44.3.115

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참고문헌

키워드

DiagnosisReviewResearchDiatomsDrowning
주제분류기타의약학

초록

The forensic significance of the diatom test in diagnosing death due to drowning has been controversial for a long time as diatoms are detected in the non-drowning cases as well. This false positivity may originate from the antemortem as well as postmortem penetration of the diatoms into the bodies. Another problem with the diatom test is the lack of standardization among the different kinds of diatom extraction and identification methods. The author first reviewed the progress in the methods used in the diatom test and then studied the historical arguments in the diagnosis of drowning. Lastly, the research trend in the diatom test since 2000 was evaluated by classifying the manuscripts searched using the keyword “drowning and diatom” on PubMed. Recent studies tend to support the reliability of the diatom test in the diagnosis of drowning when quantitative and qualitative analyses are performed properly with maximum possible reduction in contamination during the preparation of the test samples.

Abstract

The forensic significance of the diatom test in diagnosing death due to drowning has been controversial for a long time as diatoms are detected in the non-drowning cases as well. This false positivity may originate from the antemortem as well as postmortem penetration of the diatoms into the bodies. Another problem with the diatom test is the lack of standardization among the different kinds of diatom extraction and identification methods. The author first reviewed the progress in the methods used in the diatom test and then studied the historical arguments in the diagnosis of drowning. Lastly, the research trend in the diatom test since 2000 was evaluated by classifying the manuscripts searched using the keyword “drowning and diatom” on PubMed. Recent studies tend to support the reliability of the diatom test in the diagnosis of drowning when quantitative and qualitative analyses are performed properly with maximum possible reduction in contamination during the preparation of the test samples.

참고문헌 (51)

  1. Saukko P, "Knight's forensic pathology", pp.399~413 (2016), CRC Press
  2. 장의경, "무기소재 및 생물환경 소재로서의 규조류 활용 연구 동향", KSBB Journal, 29(1), pp.9~21 (2014), 한국생물공학회
  3. Lunetta P, "Scanning and transmission electron microscopical evidence of the capacity of diatoms to penetrate the alveolo-capillary barrier in drowning", Int J Legal Med, 111, pp.229~237 (1998)
  4. Timperman J, "The diagnosis of drowning : a review", Forensic Sci, 1, pp.397~409 (1972)
  5. Pollanen MS, "The diagnostic value of the diatom test for drowning, II. Validity: analysis of diatoms in bone marrow and drowning medium", J Forensic Sci, 42, pp.286~290 (1997)
  6. Lin CY, "Diatomological investigation in sphenoid sinus fluid and lung tissue from cases of suspected drowning", Forensic Sci Int, 244, pp.111~115 (2014)
  7. Ludes B, "Application of a simple enzymatic digestion method for diatom detection in the diagnosis of drowning in putrified corpses by diatom analysis", Int J Legal Med, 107, pp.37~41 (1994)
  8. Ming M, "Evaluation of four digestive methods for extracting diatoms", Forensic Sci Int, 170, pp.29~34 (2007)
  9. Sidari L, "Diatom test with Soluene-350 to diagnose drowning in sea water", Forensic Sci Int, 103, pp.61~65 (1999)
  10. DiGiancamillo A, "Diatom extraction with HCl from animal tissues: a technical note", Leg Med (Tokyo), 13, pp.268~271 (2011)
  11. Krstic S, "Diatoms in forensic expertise of drowning: a Macedonian experience", Forensic Sci Int, 127, pp.198~203 (2002)
  12. Wang H, "A simple digestion method with a Lefort aqua regia solution for diatom extraction", J Forensic Sci, 60(Suppl 1), pp.S227~S230 (2015)
  13. Kobayashi M, "Novel detection of plankton from lung tissue by enzymatic digestion method", Forensic Sci Int, 60, pp.81~90 (1993)
  14. Kakizaki E, "Simple protocol for extracting diatoms from lung tissues of suspected drowning cases within 3h : first practical application", Forensic Sci Int, 251, pp.179~185 (2015)
  15. Kakizaki E, "A new enzymatic method for extracting diatoms from organs of suspected drowning cases using papain: optimal digestion and first practical application", Forensic Sci Int, 297, pp.204~216 (2019)
  16. Fukui Y, "A new method for detecting diatoms in human organs", Forensic Sci Int, 16, pp.67~74 (1980)
  17. Yange L, "Development of can for destruction of organic material in use for forensic diatom examination", Forensic Sci Int, 101, pp.163~166 (1999)
  18. Hu S, "Detection of diatoms in water and tissues by combination of microwave digestion, vacuum filtration and scanning electron microscopy", Forensic Sci Int, 226, pp.e48~e51 (2013)
  19. 홍정원, "가거도 인근 해수에서의 플랑크톤 분포와 분석방법에 따른 검출률의 차이 비교", 대한법의학회지, 36(2), pp.144~150 (2012), 대한법의학회[DOI]
  20. Zhao J, "Microwave digestion: vacuum filtrationautomated scanning electron microscopy as a sensitive method for forensic diatom test", Int J Legal Med, 127, pp.459~463 (2013)
  21. Zhao J, "Application of the microwave digestion-vacuum filtration-automated scanning electron microscopy method for diatom detection in the diagnosis of drowning", J Forensic Leg Med, 33, pp.125~128 (2015)
  22. Hurlimann J, "Diatom detection in the diagnosis of death by drowning", Int J Legal Med, 114, pp.6~14 (2000)
  23. Bortolotti F, "Objective diagnosis of drowning by the "diatom test": a critical review", Forensic Sci Rev, 16, pp.135~148 (2004)
  24. "ADIAC: Automatic Diatom Identification and Classification" (1999)[URL]
  25. Zhou Y, "Digital whole-slide image analysis for automated diatom test in forensic cases of drowning using a convolutional neural network algorithm", Forensic Sci Int, 302, pp.109922~ (2019)
  26. Auer A, "Diatoms and drowning", Z Rechtsmed, 101, pp.87~98 (1988)
  27. Farrugia A, "Diagnostic of drowning in forensic medicine. In: Vieira DN, ed. Forensic medicine from old problems to new challenges", Rijeka: Tech, pp.53~60 (2011)
  28. Zhao J, "A quantitative comparison analysis of diatoms in the lung tissues and the drowning medium as an indicator of drowning", J Forensic Leg Med, 42, pp.75~78 (2016)
  29. Shen X, "Analysis of false-positive results of diatom test in the diagnosis of drowning-would not be an impediment", Int J Legal Med, 133, pp.1819~1824 (2019)
  30. Ludes B, "Continuous river monitoring of the diatoms in the diagnosis of drowning", J Forensic Sci, 41, pp.425~428 (1996)
  31. Ludes B, "Diatom analysis in victim's tissues as an indicator of the site of drowning", Int J Legal Med, 112, pp.163~166 (1999)
  32. Seo Y, "A simple DNA coprecipitation method for the detection of diatoms in heart blood", Forensic Sci Int, 232, pp.154~159 (2013)
  33. He F, "A novel PCR-DGGE-based method for identifying plankton 16S rDNA for the diagnosis of drowning", Forensic Sci Int, 176, pp.152~156 (2008)
  34. Li Z, "Barcoding for diatoms in the Yangtze River from the morphological observation and 18S rDNA polymorphic analysis", Forensic Sci Int, 297, pp.81~89 (2019)
  35. Spitz WU, "The significance of diatoms in the diagnosis of death by drowning", J Forensic Sci, 9, pp.11~18 (1964)
  36. Pollanen MS, "The diagnostic value of the diatom test for drowning, I. Utility: a retrospective analysis of 771 cases of drowning in Ontario, Canada", J Forensic Sci, 42, pp.281~285 (1997)
  37. Gylseth B, "Diatoms in lung tissue", Lancet, 2, pp.1375~ (1979)
  38. Foged N, "Diatoms and drowning : once more", Forensic Sci Int, 21, pp.153~159 (1983)
  39. Neidhart DA, "The significance of diatom demonstration in the diagnosis of death by drowning", Am J Clin Pathol, 48, pp.377~382 (1967)
  40. Peabody AJ, "Diatoms in forensic science", J Forensic Sci Soc, 17, pp.81~87 (1978)
  41. Bortolotti F, "Testing the specificity of the diatom test: search for false-positives", Med Sci Law, 51(Suppl), pp.S7~S10 (2011)
  42. Lunetta P, "False-positive diatom test: a real challenge? A post-mortem study using standardized protocols", Leg Med (Tokyo), 15, pp.229~234 (2013)
  43. Yen LY, "Prevalence of diatom frustules in nonvegetarian foodstuffs and its implications in interpreting identification of diatom frustules in drowning cases", Forensic Sci Int, 170, pp.1~7 (2007)
  44. Langer AM, "Inorganic particles in cigars and cigar smoke", Science, 174, pp.585~587 (1971)
  45. Calder IM, "An evaluation of the diatom test in deaths of professional divers", Med Sci Law, 24, pp.41~46 (1984)
  46. Taylor JJ, "Diatoms and drowning: a cautionary case note", Med Sci Law, 34, pp.78~79 (1994)
  47. Pachar JV, "The diagnosis of drowning by quantitative and qualitative diatom analysis", Med Sci Law, 33, pp.291~299 (1993)
  48. Antonenko NE, "Diatom analysis in the determination of death by drowning", Can Soc Forensic Sci J, 20, pp.1~11 (1987)
  49. Houck MM, "The science of crime scenes", pp.323~339 (2018), Academic Press
  50. Piette MH, "Drowning: still a difficult autopsy diagnosis", Forensic Sci Int, 163, pp.1~9 (2006)
  51. Stephenson L, "The persistent problem of drowning : a difficult diagnosis with inconclusive tests", J Forensic Leg Med, 66, pp.79~85 (2019)

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