나이트로벤젠다이아조늄 양이온의 화학 및 전기화학 반응을 이용한 실리콘 표면상으로의 단백질 고정
Immobilization of Proteins on Silicon Surfaces Using Chemical and Electrochemical Reactions of Nitrobenzenediazonium Cations
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전기화학 반응을 이용한 실리콘 표면상으로의 단백질 고정을 연구하였다. 이를 위해Nitrobenzendiazonium(NiBD) 양이온을 화학적 환원반응을 통해 수식하고 수식된 실리콘 표면을전기화학적으로 다시 환원시켜 나이트로 기능기를 일차아민 기능기로 활성화하여 단백질 고정에이용하였다. 활성화 된 표면에 금 나노입자를 고정하여 일차 아민 생성을 확인하였다. 또한 이방법을 응용하여 실리콘 나노선 어레이 중 선택된 나노선 만을 활성화하고 단백질을 선택적으로고정하는 연구를 수행하였다. 이 연구를 통하여 NiBD 양이온의 화학 및 전기화학 반응이 실리콘나노선 표면으로 단백질의 선택적 고정화에 유용하게 사용될 수 있음을 보였다.
Abstract
The immobilization of proteins on silicon surfaces using electrochemical reaction has been studied. Chemical deposition of nitrobenzendiazonium (NiBD) cations is employed to modify silicon surfaces. Electrochemical reduction of nitro-group to primary amine-group have been conducted on the modified surfaces to activate silicon surfaces for the protein immobilization. Attachment of gold nanoparticles was used to prove the reduction. The current method was applied to selective activation of a silicon nanowire and immobilize proteins on the selected nanowire. It has been demonstrated that the use of chemical and electrochemical reaction NiBD is efficient for the selective immobilization of proteins on silicon nanowire surfaces.
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