네모파 흡착 벗김 전압전류법에 의한 플루오로퀴놀론 계 항생제의 검출
Determination of Fluoroquinolone Antibacterial Agents by Square Wave Adsorptive Stripping Voltammetry
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초록
플루오로퀴놀론 계 항생제들의 전기화학적 거동을 카본 페이스트 전극 (carbon paste electrode ;CPE)을 사용하여 순환 전압전류법 (cyclic voltammetry)과 네모파 흡착 벗김 전압전류법 (square wave adsorptive stripping voltammetry)으로 연구하였다. Enrofloxacin (ENR), Norfloxacin (NOR),Ciprofloxacin (CIP), Ofloxacin (OFL), Levofloxacin (LEV) 등 5가지 플루오로퀴놀론 계 항생제에대한 전기화학적 분석을 수행하였다. pH 4.5인 아세트산 완충 용액 (acetate buffer) 에서 플루오로퀴놀론 계 항생제의 산화 전위는 Ag/AgCl 기준 전극에 대하여 각각 ENR : 0.952V, NOR : 1.052 V,CIP : 1.055 V, OFL : 0.983 V, LEV : 0.990 V 의 값을 나타내었으며, 네모파 흡착 벗김 전압전류법에 의한 산화 전류는 0.2 μM에서 1 μM 사이의 농도영역에서 각 항생제의 농도와 선형을나타내었다.
Abstract
Electrochemical behavior of fluoroquinolone antibacterial agents on carbon paste electrode (CPE) were investigated by cyclic voltammetry and square wave adsorptive stripping voltammetry. The fluoroquinolone antibacterial agents tested in this study were Enrofloxacin (ENR), Norfloxacin (NOR), Ciprofloxacin (CIP), Ofloxacin (OFL) and Levofloxacin (LEV). In acetate buffer at pH 4.5, the oxidation peak potentials of the fluoroquinolone antibacterial agents of ENR, NOR, CIP, OFL, and LEV were 0.952 V, 1.052 V, 1.055 V, 0.983 V, and 0.990 V (vs. Ag/AgCl), respectively. And their oxidation peak currents from square wave adsorptive stripping voltammograms are proportional to the concentration of each antibacterial agent over the range from 0.2 μM to 1 μM.
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