HPLC를 이용한 차와 커피에 함유된 카페인의 함량 조사와 카페인이 흰 쥐의 행동에 미치는 영향 연구
Evaluation of the Caffeine Contents in Tea and Coffee by HPLC and Effect of Caffeine on Behavior in Rats
1
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Different studies have been conducted regarding caffeine as psychostimulant drugs and its effects as well as determination in various materials. Since, coffee and tea are most drinkable beverage in the world and their major constituent is caffeine. So, analysis of the effect of equal amount of caffeine in coffee and tea with respect to SD rats was studied. The present methodology was purposed to determine and validate caffeine amount in different brands of coffee and tea,and canvass locomotor and behavioral patterns of SD rats after administrating coffee and tea orally consisting of same amount of caffeine (10, 30 mg/kg). Determination of caffeine in different brands of coffee and tea and validation of caffeine were evaluated using HPLC. Depending upon different brands of tea and coffee, caffeine amount was altered. Meanwhile, amount of caffeine in tea was directly proportional to the temperature of liquid. Coffee and tea (Instant Maxim original coffee® and earl grey black tea®) consisting 10, 30 mg/kg of caffeine were studied in SD rats for locomotor activity and behavioral patterns using Tru Scan 99. The locomotor activities of SD rats were increased after administration of coffee and tea consisting caffeine compared to water. The coffee consisted of higher amount of caffeine exhibited steep movement of SD rats. Similarly, rearing and scratching of SD rats as frequency and duration were increased in coffee and tea consisting caffeine compared with water. The study revealed that coffee and tea had effects in locomotor activity and behavioral patterns of SD rats
Abstract
Different studies have been conducted regarding caffeine as psychostimulant drugs and its effects as well as determination in various materials. Since, coffee and tea are most drinkable beverage in the world and their major constituent is caffeine. So, analysis of the effect of equal amount of caffeine in coffee and tea with respect to SD rats was studied. The present methodology was purposed to determine and validate caffeine amount in different brands of coffee and tea,and canvass locomotor and behavioral patterns of SD rats after administrating coffee and tea orally consisting of same amount of caffeine (10, 30 mg/kg). Determination of caffeine in different brands of coffee and tea and validation of caffeine were evaluated using HPLC. Depending upon different brands of tea and coffee, caffeine amount was altered. Meanwhile, amount of caffeine in tea was directly proportional to the temperature of liquid. Coffee and tea (Instant Maxim original coffee® and earl grey black tea®) consisting 10, 30 mg/kg of caffeine were studied in SD rats for locomotor activity and behavioral patterns using Tru Scan 99. The locomotor activities of SD rats were increased after administration of coffee and tea consisting caffeine compared to water. The coffee consisted of higher amount of caffeine exhibited steep movement of SD rats. Similarly, rearing and scratching of SD rats as frequency and duration were increased in coffee and tea consisting caffeine compared with water. The study revealed that coffee and tea had effects in locomotor activity and behavioral patterns of SD rats
참고문헌 (22)
- Huck CW, "Analysis of caffeine, theobromine and theophylline in coffee by near infrared spectroscopy (NIRS) compared to high-performance liquid chromatography (HPLC) coupled to mass spectrometry", Analytica Chimica Acta, 538, pp.195~203 (2005)
- Nakagawa M, "Constituents in tea leaf and their contribution to the taste of green tea liquor", Jpn Agric Res Q, 5(3), pp.43~47 (1970)
- Bunker ML, "Caffeine content of common beverages", J Am Diet Assoc, 74(1), pp.28~32 (1979)
- Chou T, "Wake up and smell the coffee. Caffeine, coffee, and the medical consequences", West J Med, 157(5), pp.544~553 (1992)
- Lorist MM, "Caffeine, fatigue, and cognition", Brain Cogn, 53(1), pp.82~94 (2003)
- Ferré S, "An update on the mechanisms of the psychostimulant effects of caffeine", J Neurochem, 105(4), pp.1067~1079 (2008)
- Richardson NJ, "Mood and performance effects of caffeine in relation to acute and chronic caffeine deprivation", Pharmacol Biochem Behav, 52(2), pp.313~320 (1995)
- Knutti R, "Effect of pregnancy on the pharmacokinetics of caffeine", Eur J Clin Pharmacol, 21(2), pp.121~126 (1981)
- Fenster L, "Caffeine consumption during pregnancy and fetal growth", Am J Public Health, 81(4), pp.458~461 (1991)
- Tyrala EE, "Caffeine secretion into breast milk", Arch Dis Child, 54(10), pp.787~789 (1979)
- Yoon JO, "Amount of caffeine in caffeine containing products, and the pattern of caffeine consumption", Kor J Clin Pharm, 3(1), pp.21~30 (1993)
- Kim HY, "Development of analysis method of caffeine and content survey in commercial foods by HPLC", Korean J Food Sci Technol, 31(6), pp.1471~1476 (1999)
- Antoniou K, "d-amphetamine, cocaine and caffeine: A comparative study of acute effects on locomotor activity and behavioural patterns in rats", Neurosci Biobehav Rev, 23(2), pp.189~196 (1998)
- Yong CS, "Bioequivalence of Cilostan tablet (Korea United Pharmaceutical Co.) to Pletaal tablet (cilostazol 100 mg)", J Kor Soc Health-syst Pharm, 20(2), pp.239~246 (2003)
- 성민경, "인체혈청 중 펜톡시필린의 정량을 위한 HPLC 분석법의 검증 및 단일용량 투여에 의한 약물동태", 한국임상약학회지, 15(1), pp.46~49 (2005), 한국임상약학회
- US Department of Health and Human Services, "Guidance for industry: Bioanalytical method validation", ~ (2001)
- McOmish CE, "Phospholipase C-β1 knockout mice exhibit endophenotypes modeling schizophrenia which are rescued by environmental enrichment and clozapine administration", Mol Psychiatry, 13(7), pp.661~672 (2008)
- Yoon MH, "A evaluation of the caffeine contents in commercial foods", J Fd Hyg Safety, 16(4), pp.295~299 (2001)
- Rogers PJ, "Time for tea: mood, blood pressure and cognitive performance effects of caffeine and theanine administered alone and together", Psychopharmacology (Berl), 195(4), pp.569~577 (2008)
- Kakuda T, "Inhibiting effects of theanine on caffeine stimulation evaluated by EEG in the rat", Biosci Biotechnol Biochem, 64(2), pp.287~293 (2000)
- Lu K, "The acute effects of Ltheanine in comparison with alprazolam on anticipatory anxiety in humans", Hum Psychopharmacol, 19(7), pp.457~465 (2004)
- Juneja LR, "L-theanine-a unique amino acid of green tea and its relaxation effect in humans", Trends Food Sci Technol, 10(6-7), pp.199~204 (1999)